XPNPEP3 antibody
Principal name
XPNPEP3 antibody
Alternative names for XPNPEP3 antibody
X-Pro aminopeptidase 3, APP3, Probable Xaa-Pro aminopeptidase 3
SwissProt ID
Q9NQH7 (Human)
Gene ID
63929 (XPNPEP3)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of XPNPEP3 antibody
- Kit Component:
- - KN200888G1, XPNPEP3 gRNA vector 1 in pCas-Guide vector
- KN200888G2, XPNPEP3 gRNA vector 2 in pCas-Guide vector
- KN200888D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
LOC63929 MaxPab polyclonal antibody. Western Blot analysis of LOC63929 expression in human liver.
Western Blot analysis of XPNPEP3 expression in transfected 293T cell line (H00063929-T01) by XPNPEP3 MaxPab polyclonal antibody.Lane 1: LOC63929 transfected lysate(55.77 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-XPNPEP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; XPNPEP3 antibody - N-terminal region (ARP57593_P050) in Human MCF-7 cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:XPNPEP3. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; XPNPEP3 antibody - N-terminal region (ARP57593_P050) in Hum. Adult Placenta cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:XPNPEP3. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. XPNPEP3 is supported by BioGPS gene expression data to be expressed in 721_B.; XPNPEP3 antibody - N-terminal region (ARP57593_P050) in Human 721_B cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503304)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503304)
Anti-XPNPEP3 mouse monoclonal antibody (TA503304) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP3(RC200888).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP3 (RC200888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP3 monoclonal antibody.
HEK293T cells transfected with either RC200888 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-XPNPEP3 antibody(TA503304), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503339)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503339)
Anti-XPNPEP3 mouse monoclonal antibody (TA503339) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP3(RC200888).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP3 (RC200888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP3 monoclonal antibody.
HEK293T cells transfected with either RC200888 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-XPNPEP3 antibody(TA503339), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPNPEP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503349)
Anti-XPNPEP3 mouse monoclonal antibody (TA503349) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP3(RC200888).
Immunofluorescent staining of HeLa cells using anti-XPNPEP3 mouse monoclonal antibody (TA503349).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP3 (RC200888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP3 monoclonal antibody.
HEK293T cells transfected with either RC200888 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-XPNPEP3 antibody(TA503349), and then analyzed by flow cytometry.
Sample(30 ug whole cell lysate). A:A431. 7.5% SDS PAGE. TA307885 diluted at 1:500
Immunofluorescence analysis of paraformaldehyde-fixed A549, using XPNPEP3(TA307885) antibody at 1:200 dilution.
Predicted band size: 57 kDa. Positive control: MCF7 and A431 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: MCF7 cell lysate Lane2: A431 cell lysate Lysates: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm. Positive control: Lymphoma tissue. Recommended dilution: 1/25-100 The image is immunohistochemistry of paraffin-embedded Lymphoma tissue using XPNPEP3 (TA321891 antibody at dilution 1/40. (Original magnification:× 200)
Predicted cell location: Cytoplasm. Positive control: Lymphoma tissue. Recommended dilution: 1/25-100 The image is treated with the synthetic peptide. (Original magnification:×200)
WB Suggested Anti-XPNPEP3 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate
Host:Rabbit; Target Name:XPNPEP3; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:XPNPEP3; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlXPNPEP3 is supported by BioGPS gene expression data to be expressed in 721_B
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503304 | XPNPEP3 antibody |
||||||
| XPNPEP3 mouse monoclonal antibody, clone OTI5G2 (formerly 5G2) | |||||||
 |
Mouse | IgG2b | OTI5G2 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
||||||
| TA503339 | XPNPEP3 antibody |
||||||
| XPNPEP3 mouse monoclonal antibody, clone OTI4B8 (formerly 4B8) | |||||||
 |
Mouse | IgG2a | OTI4B8 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA503349 | XPNPEP3 antibody |
||||||
| XPNPEP3 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) | |||||||
 |
Mouse | IgG2b | OTI2D5 | Purified | Can, Hu, Mky, Ms, Rt | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA307885 | XPNPEP3 antibody |
||||||
| Rabbit polyclonal antibody to XPNPEP3 (X-prolyl aminopeptidase (aminopeptidase P) 3, putative) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA321891 | XPNPEP3 antibody |
||||||
| Anti-XPNPEP3 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300888 | XPNPEP3 |
||||
| Recombinant protein of human X-prolyl aminopeptidase (aminopeptidase P) 3, putative (XPNPEP3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402903 | XPNPEP3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
