XRCC1 antibody
Principal name
XRCC1 antibody
Alternative names for XRCC1 antibody
DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)
Background of XRCC1 antibody
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.
Immunohistochemistry (IHC) analyzes of XRCC1 antibody (Cat.-No.: AP06480PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of XRCC1 antibody (Cat.-No.: AP06480PU-N) in extracts from Jurkat cells.
Figure 1. Western blot of HT29 cell lysate using anti-XRCC1 antibody (AM05323PU-N).
Immunoperoxidase of purified MaxPab antibody to XRCC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to XRCC1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of XRCC1 expression in transfected 293T cell line (H00007515-T01) by XRCC1 MaxPab polyclonal antibody.Lane 1: XRCC1 transfected lysate(69.63 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of XRCC1 expression in HT-29 cell lysate using XRCC1 monoclonal antibody, clone 144 ( Cat # MAB1792 ).
Formalin-fixed, paraffin embedded human testis stained with XRCC1 monoclonal antibody, clone 33-2-5 ( Cat # MAB3419 )
Immunoprecipitation ( Native verified ) of LS174T cell line of XRCC1 monoclonal antibody, clone 33-2-5 ( Cat # MAB3419 ). Use Protein A. Use antibody at 2 µg/mg protein lysate.
XRCC1 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC1 expression in mouse kidney.
Western Blot analysis of XRCC1 expression in transfected 293T cell line (H00007515-T02) by XRCC1 MaxPab polyclonal antibody.Lane 1: XRCC1 transfected lysate(69.50 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of XRCC1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of XRCC1 expression in MCF7 (A); U2OS (B); A431 (C); SHSY5Y (D); Saos2 (E) whole cell lysates.
Anti-XRCC1 mouse monoclonal antibody (TA500847) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1(RC204952).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-XRCC1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Flow cytometric Analysis of Hela cells, using anti-XRCC1 antibody(TA500879),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-XRCC1 antibody(TA500879),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500880, Dilution 1:50)
Anti-XRCC1 mouse monoclonal antibody (TA500880) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1(RC204952).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Anti-XRCC1 mouse monoclonal antibody (TA500881) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1(RC204952).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-XRCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500886)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-XRCC1 monoclonal antibody at 1:200 dilution.
Flow cytometric Analysis of Jurkat cells, using anti-XRCC1 antibody(TA500886),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC204952 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-XRCC1 antibody(TA500886), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-XRCC1 rabbit polyclonal antibody. (TA590221)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (RC204952, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XRCC1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: XRCC1 Antibody
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XRCC1 mouse monoclonal antibody (UM500037). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XRCC1 (Clone UMAB37) very specifically recognizes XRCC1 antigen on OriGene protein microarray chip.
Western blot analysis of extracts from Jurkat cells, using XRCC1 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2), HuvEc cells (Lane 3) and COS7 cells (Lane 4), using XRCC1 Antibody. The lane on the left is treated with systhesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using XRCC1 antibody.The picture on the right is treated with the synthesized peptide.
XRCC1 Antibody (Center) (Cat. #TA324813) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).
XRCC1 Antibody (Center) (Cat. #TA324813) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Immunofluorescent analysis of A549 cells, using XRCC1 Antibody (Center) (Cat. #TA324813). TA324813 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
XRCC1 Antibody (Center) (Cat. #TA324813) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Host: Rabbit; Target Name: XRCC1; Sample Tissue: MCF7 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Western blot detection of XRCC1 in Raji,K562,Hela,Jurkat and THP-1 cell lysates using XRCC1 mouse mAb (1:1000 diluted).Predicted band size:82KDa.Observed band size:82KDa.
Western blot analysis on Jurkat,COS7 and HuvEc cell lysate using XRCC1 Antibody
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500037 | XRCC1 antibody |
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| anti-XRCC1 mouse monoclonal antibody, clone UMAB40 | |||||||
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Mouse | IgG1 | UMAB40 | Purified | Hu, Mky |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
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| UM570037 | XRCC1 antibody |
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| anti-XRCC1 mouse monoclonal antibody, clone UMAB40 | |||||||
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Mouse | IgG1 | UMAB40 | Purified | Hu, Mky |
30 µl /
€180.00
|
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| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
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| TA500879 | XRCC1 antibody |
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| XRCC1 mouse monoclonal antibody, clone OTI2G8 (formerly 2G8) | |||||||
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Mouse | IgG1 | OTI2G8 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA500880 | XRCC1 antibody |
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| anti-XRCC1 mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | |||||||
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Mouse | IgG1 | OTI2F8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
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| TA500886 | XRCC1 antibody |
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| XRCC1 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8) | |||||||
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Mouse | IgG1 | OTI2D8 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA312309 | XRCC1 antibody |
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| Rabbit polyclonal anti-XRCC1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA324813 | XRCC1 antibody |
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| Rabbit polyclonal XRCC1 Antibody (Center) | |||||||
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Rabbit | IgG | Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA347071 | XRCC1 antibody |
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| Mouse Monoclonal XRCC1 Antibody | |||||||
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Mouse | IgG1 | 3F6-G7-F7 | Purified | WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA347508 | XRCC1 antibody |
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| Rabbit Polyclonal Anti-XRCC1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA500847 | XRCC1 antibody |
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| anti-XRCC1 mouse monoclonal antibody, clone OTI3C3 (formerly 3C3) | |||||||
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Mouse | IgG1 | OTI3C3 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA500881 | XRCC1 antibody |
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| anti-XRCC1 mouse monoclonal antibody, clone OTI2E8 (formerly 2E8) | |||||||
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Mouse | IgG1 | OTI2E8 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA500889 | XRCC1 antibody |
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| XRCC1 mouse monoclonal antibody, clone OTI2H8 (formerly 2H8) | |||||||
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Mouse | IgG1 | OTI2H8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP06480PU-N | XRCC1 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304952 | XRCC1 |
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| Recombinant protein of human X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209603L2V | Lenti ORF particles, Xrcc1 (GFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 1 (Xrcc1), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,370.00
|
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| OriGene Technologies, Inc. | ||
| MR209603L1V | Lenti ORF particles, Xrcc1 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 1 (Xrcc1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,230.00
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| OriGene Technologies, Inc. | ||