XRCC3 antibody
Principal name
XRCC3 antibody
Alternative names for XRCC3 antibody
DNA repair protein XRCC3, X-ray repair cross-complementing protein 3
Ncbi ID
9606, NP_001093588.1, NP_001093588, NM_005432, NP_005423.1, NP_083151.1, NP_005423, BC043073, NM_028875, NM_001100118, NM_001100119
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of XRCC3 antibody
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.
General readings
Tebbs RS. et al. (1995) Proc Natl Acad Sci U S A. 92:6354-6358.Miller KA. et al. (2002) J Biol Chem. 277:8406-8411.
Western Blot analysis of XRCC3 expression in transfected 293T cell line (H00007517-T01) by XRCC3 MaxPab polyclonal antibody.Lane 1: XRCC3 transfected lysate(38.06 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analyzes of XRCC3 antibody (Cat.-No.: AP06482PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of XRCC3 antibody (Cat.-No.: AP06482PU-N) in extracts from 293 cells treated with serum 10% 15'.
XRCC3 detected in HEK293 lysates using a 1 : 1,000 dilution of XRCC3 monoclonal antibody, clone 10F1/6 ( Cat # MAB2393 ). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School.
XRCC3 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in human liver.
Western Blot analysis of XRCC3 expression in transfected 293T cell line (H00007517-T02) by XRCC3 MaxPab polyclonal antibody.Lane 1: XRCC3 transfected lysate(37.80 KDa).Lane 2: Non-transfected lysate.
XRCC3 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in K-562.
Western blot analysis of XRCC3 in HTC116 and HeLa whole cell extracts with XRCC3 polyclonal antibody at 1 : 2500 ( Cat# PAB11977 ).
XRCC3 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in K-562.
XRCC3 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in human liver.
Immunoprecipitation of XRCC3 transfected lysate using anti-XRCC3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with XRCC3 MaxPab mouse polyclonal antibody (B01) (H00007517-B01).
Human ACHN; WB Suggested Anti-XRCC3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: ACHN Whole Cell; XRCC3 antibody - C-terminal region (ARP61274_P050) in Human ACHN cells using Western Blot
Human MCF7; Host:Rabbit. Target Name:XRCC3. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. XRCC3 is supported by BioGPS gene expression data to be expressed in MCF7.; XRCC3 antibody - C-terminal region (ARP61274_P050) in Human MCF7 cells using Western Blot
Human ACHN; WB Suggested Anti-XRCC3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: ACHN Whole Cell; XRCC3 antibody - middle region (ARP61275_P050) in Human ACHN cells using Western Blot
Immunohistochemical analysis of XRCC3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of XRCC3 expression in HeLa (A); H1299 (B); U2OS (C); HepG2 (D) whole cell lysates.
ELISA: XRCC3 Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC3 (RC200326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC3 (RC200326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC3 (RC200326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC3 (RC200326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC3.(1:2000)
Western Blot: XRCC3 Antibody TA301486 - Detection of XRCC3 (38 kDa) from Hela cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
XRCC3 detected in HEK293 lysates using a 1:1,000 dilution
XRCC3 antibody detects XRCC3 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of DDDDK-human XRCC3-transfected 293T cells. 10 % SDS-PAGE. XRCC3 antibody (TA308545) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC3(TA308545) antibody at 1:200 dilution.
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using XRCC3 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS-7 cells, using XRCC3 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using XRCC3 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using XRCC3 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of HL60 cell lines, using XRCC3 antibody.
Immunofluorescence analysis of U2OS cell using XRCC3 antibody. Blue: DAPI for nuclear staining.
Western blot analysis on HepG2 cell lysate using XRCC3 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301486 | XRCC3 antibody |
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| Rabbit Polyclonal Antibody against XRCC3 | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| TA301487 | XRCC3 antibody |
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| Mouse Monoclonal Antibody against XRCC3 (10F1/6) | |||||||
 |
Mouse | IgG1 | 10F1/6 | Purified | Hu, Ms | WB |
0.1 ml /
€450.00
|
| OriGene Technologies, Inc. | |||||||
| TA308545 | XRCC3 antibody |
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| Rabbit polyclonal antibody to XRCC3 (X-ray repair complementing defective repair in Chinese hamster cells 3) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA312227 | XRCC3 antibody |
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| Rabbit polyclonal anti-XRCC3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA312228 | XRCC3 antibody |
||||||
| Rabbit polyclonal anti-XRCC3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA327290 | XRCC3 antibody |
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| Rabbit anti-XRCC3 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA347509 | XRCC3 antibody |
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| Rabbit Polyclonal Anti-XRCC3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590205 | XRCC3 antibody |
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| Rabbit Polyclonal XRCC3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA804755 | XRCC3 antibody |
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| XRCC3 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8) | |||||||
 |
Mouse | IgG1 | OTI4C8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804761 | XRCC3 antibody |
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| XRCC3 mouse monoclonal antibody, clone OTI4F3 (formerly 4F3) | |||||||
 |
Mouse | IgG1 | OTI4F3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804763 | XRCC3 antibody |
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| XRCC3 mouse monoclonal antibody, clone OTI5B11 (formerly 5B11) | |||||||
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Mouse | IgG1 | OTI5B11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804776 | XRCC3 antibody |
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| XRCC3 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
 |
Mouse | IgG1 | OTI1C1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP06482PU-N | XRCC3 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760055 | XRCC3 (full length, N-term HIS tag, transcript variant 2) |
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| Recombinant protein of human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760503 | XRCC3 (full length, N-term HIS tag, transcript variant 3) |
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| Purified recombinant protein of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202365L2V | Lenti ORF particles, Xrcc3 (GFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (cDNA clone MGC:57978 IMAGE:6404521),, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR219979L2V | Lenti ORF particles, Xrcc3 (GFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202365L1V | Lenti ORF particles, Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (cDNA clone MGC:57978 IMAGE:6404521),, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
| MR219979L1V | Lenti ORF particles, Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
