XRCC4 antibody
Principal name
XRCC4 antibody
Alternative names for XRCC4 antibody
DNA repair protein XRCC4, X-ray repair cross-complementing protein 4
Ncbi ID
NP_003392.1, NP_071801, 9606, NP_003392, NP_072044, NP_082288.1, NM_028012, NM_022406, NM_003401, NM_022550
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of XRCC4 antibody
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.
Western blot(WB) analysis of XRCC4 antibody (Cat.-No.: AP06483PU-N) in extracts from COLO205 cells.
Human Placenta; WB Suggested Anti-XRCC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Human Placenta; XRCC4 antibody - middle region (ARP34125_P050) in Human Placenta cells using Western Blot
Western Blot analysis of XRCC4 expression in transfected 293T cell line (H00007518-T01) by XRCC4 MaxPab polyclonal antibody.Lane 1: XRCC4 transfected lysate(36.96 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of purified MaxPab antibody to XRCC4 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to XRCC4 on HeLa cell. [antibody concentration 10 ug/ml]
XRCC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC4 expression in mouse liver.
Immunoprecipitation of XRCC4 transfected lysate using anti-XRCC4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with XRCC4 MaxPab mouse polyclonal antibody (B01) (H00007518-B01).
XRCC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of XRCC4 expression in mouse liver.
Western Blot analysis of XRCC4 expression in transfected 293T cell line (H00007518-T02) by XRCC4 MaxPab polyclonal antibody.Lane 1: XRCC4 transfected lysate(38.30 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of XRCC4 expression in HeLa (A); MCF7 (B); NIH3T3 (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-XRCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500650, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (RC218029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.
ELISA: XRCC4 Antibody - Affinity Purified
Western blot analysis of extracts from COLO205 cells, using XRCC4 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines , using XRCC4 antibody.
Sample (30 ug of whole cell lysate). A: Jurkat. B: K562. C: HL-60. 10% SDS PAGE. TA308834 diluted at 1:1000.
XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. XRCC4 antibody (TA308834) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4(TA308834) antibody at 1:200 dilution.
WB Suggested Anti-XRCC4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Human Placenta
Western blot analysis on COLO205 cell lysate using XRCC4 Antibody
Western blot analysis of XRCC4 expression in Hela (A); 293T (B) whole cell lysates.
Immunohistochemical analysis of XRCC4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation of XRCC4 from 0.5mg Hela whole cell extract lysate; using Anti-XRCC4 Antibody.
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318029 | XRCC4 (transcript variant 3) |
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| Recombinant protein of human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
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| TP312684 | XRCC4 (transcript variant 1) |
||||
| Recombinant protein of human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||