YES1 antibody
Principal name
YES1 antibody
Alternative names for YES1 antibody
YES, p61-Yes, c-Yes, Proto-oncogene tyrosine-protein kinase Yes
Ncbi ID
NP_005424.1, 9606, NP_005424, NM_005433, NP_001192061.1, NM_009535
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of YES1 antibody
- Kit Component:
- - KN319546G1, Yes1 gRNA vector 1 in pCas-Guide vector
- KN319546G2, Yes1 gRNA vector 2 in pCas-Guide vector
- KN319546D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection of endogenous yes Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab yes-2B8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D
Western blot analysis of YES1 over-expressed 293 cell line, cotransfected with YES1 Validated Chimera RNAi ( Cat # H00007525-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with YES1 monoclonal antibody (M01) clone 2F4 (Cat # H00007525-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of YES1 expression in transfected 293T cell line by YES1 monoclonal antibody (M01), clone 2F4.Lane 1: YES1 transfected lysate(59.84 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2B and YES1 Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
YES1 monoclonal antibody (M02), clone 3C6 Western Blot analysis of YES1 expression in Jurkat ( Cat # L017V1 ).
YES1 monoclonal antibody (M02), clone 3C6. Western Blot analysis of YES1 expression in Hela NE ( Cat # L013V3 ).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with YES1 polyclonal antibody ( Cat # PAB3445 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of YES1 polyclonal antibody ( Cat # PAB3445 ) in mouse kidney tissue lysate. YES1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunoperoxidase of monoclonal antibody to YES1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]
YES1 monoclonal antibody (M03), clone 2D5 Western Blot analysis of YES1 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.
YES1 monoclonal antibody (M05), clone 2A12 Western Blot analysis of YES1 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.
Western Blot analysis of YES1 expression in transfected 293T cell line (H00007525-T01) by YES1 MaxPab polyclonal antibody.Lane 1: YES1 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of YES1 expression in transfected 293T cell line (H00007525-T01) by YES1 MaxPab polyclonal antibody.Lane 1: YES1 transfected lysate(59.73 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-YES Pab in mouse kidney tissue lysate. YES (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunoprecipitation of YES1 transfected lysate using anti-YES1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with YES1 MaxPab mouse polyclonal antibody (B01) (H00007525-B01).
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of methanol-fixed HeLa, using c-Yes antibody at 1:200 dilution.
Sample (30 µg of whole cell lysate). A:NIH-3T3. 7.5% SDS PAGE. YES1 antibody diluted at 1:1000.
Western blot analysis using YES1 antibody cat.-No AM06215SU-N against truncated YES1-His recombinant protein (Lane 1) and full-length GFP-YES1(aa1-543) transfected COS7 cell lysate (Lane 2).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-YES1 rabbit polyclonal antibody. (TA590206)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YES1 (RC208432, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YES1.
ELISA: Yes1 Antibody
Predicted band size: 61 kDa. Positive control: Lncap cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06215SU-N | YES1 antibody |
||||||
 |
Mouse | IgG1 | 2F3E6 | Ascites | Hu | E, WB |
0.1 ml /
€420.00
|
| OriGene Technologies GmbH | |||||||
