ZADH1 antibody
Principal name
ZADH1 antibody
Alternative names for ZADH1 antibody
PTGR2, PRG-2, Prostaglandin-reductase 2, 15-oxoprostaglandin 13-reductase, Zinc-binding alcohol dehydrogenase domain-containing protein 1
Ncbi ID
9606, NP_689657, NP_001139626.1, NP_001139626, NP_001139627, NP_084156.2, NM_001015009, NM_152444
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ZADH1 antibody
- Kit Component:
- - KN314181G1, Ptgr2 gRNA vector 1 in pCas-Guide vector
- KN314181G2, Ptgr2 gRNA vector 2 in pCas-Guide vector
- KN314181D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wu, Y.H., et al. Structure 16(11):1714-1723 (2008).
Gao, K., et al. Int. J. Oncol. 32(6):1343-1349 (2008).
Chou, W.L., et al. J. Biol. Chem. 282(25):18162-18172 (2007).
Lamesch, P., et al. Genomics 89(3):307-315 (2007).
Zhang, L., et al. Cytogenet. Genome Res. 103 (1-2), 79-83 (2003).
Western Blot analysis of ZADH1 expression in transfected 293T cell line by ZADH1 MaxPab polyclonal antibody.Lane 1: ZADH1 transfected lysate(38.61 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-ZADH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; ZADH1 antibody - middle region (ARP52975_P050) in Human MCF-7 cells using Western Blot
Formalin fixed and paraffin embedded human liver tissue reacted with ZADH1 Antibody (N-term) Cat.-No AP53499PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining
Western blot analysis of ZADH1 Antibody (N-term) Cat.-No AP53499PU-N in 293 cell line lysates (35ug/lane).
Immunohistochemical analysis of PTGR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PTGR2 expression in MCF7 (A); PC12 (B) whole cell lysates.
Western blot analysis of extracts from HUVEC cells, K562 cells and HT-29 cells, using ZADH1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-PTGR2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: MCF7 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316144 | ZADH1 antibody |
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| Rabbit polyclonal anti-PTGR2 (ZADH1) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP53499PU-N | ZADH1 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309182 | ZADH1 |
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| Recombinant protein of human prostaglandin reductase 2 (PTGR2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328838 | ZADH1 (transcript variant 2) |
||||
| Recombinant protein of human prostaglandin reductase 2 (PTGR2), transcript variant 2. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP328839 | ZADH1 (transcript variant 3) |
||||
| Recombinant protein of human prostaglandin reductase 2 (PTGR2), transcript variant 3. | |||||
 |
Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214345L2V | Lenti ORF particles, Ptgr2 (GFP-tagged ORF) - Rat prostaglandin reductase 2 (Ptgr2), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR214345L1V | Lenti ORF particles, Ptgr2 (Myc-DDK-tagged ORF) - Rat prostaglandin reductase 2 (Ptgr2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||