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ZBTB7B antibody

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Principal name

ZBTB7B antibody

Alternative names for ZBTB7B antibody

ZFP67, Zfp-67, ZBTB15, ZBT-15, Thpok, Th-POK, cKrox, c-Krox, Zinc finger and BTB domain-containing protein 7B, Zinc finger protein 67 homolog, Zinc finger and BTB domain-containing protein 15, T-helper-inducing POZ/Krueppel-like factor

SwissProt ID

O15156 (Human), Q64321 (Mouse)

Gene ID

51043 (ZBTB7B), 22724 (Zbtb7b)

Ncbi ID

NP_056956, 9606, NP_033591.2, NP_056956.2, NP_056956.1, NM_009565, NM_015872

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Xen (Xenopus), Bear, GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ZBTB7B antibody

Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.

General readings

Widom, R.L., et al., (1997) Gene 198 (1-2), 407-420

ARP32749_T100 - ZBTB7B
Human Lung; ZFP67 antibody - middle region (ARP32749_T100) in Human Lung cells using Immunohistochemistry
ARP32749_T100 - ZBTB7B
Human Heart; ZFP67 antibody - middle region (ARP32749_T100) in Human Heart cells using Immunohistochemistry
ARP32749_T100 - ZBTB7B
Hum. Fetal Liver; Host: Rabbit . Target Name: ZFP67 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; ZFP67 antibody - middle region (ARP32749_T100) in Hum. Fetal Liver cells using Western Blot
ARP32749_T100 - ZBTB7B
Human HepG2; WB Suggested Anti-ZFP67 Antibody Titration: 1.0-2.0ug/ml. Positive Control: HepG2 cell lysate; ZFP67 antibody - middle region (ARP32749_T100) in Human HepG2 cells using Western Blot
ARP32749_T100 - ZBTB7B
HepG2; Host: Rabbit. Target Name:ZFP67. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1.25ug/mL. Peptide Concentration: 1.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%.; ZFP67 antibody - middle region (ARP32749_T100) in HepG2 cells using Western Blot
PAB4466 - ZBTB7B
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZBTB7B polyclonal antibody ( Cat # PAB4466 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
PAB4466 - ZBTB7B
Western blot analysis of ZBTB7B polyclonal antibody ( Cat # PAB4466 ) in mouse brain tissue lysates ( 35 µg/lane ) . ZBTB7B ( arrow ) was detected using the purified ZBTB7B polyclonal antibody ( Cat # PAB4466 ) ( 1 : 60 dilution ) .
H00051043-B01 - ZBTB7B
Western Blot analysis of ZBTB7B expression in transfected 293T cell line (H00051043-T01) by ZBTB7B MaxPab polyclonal antibody.Lane 1: ZBTB7B transfected lysate(59.29 KDa).Lane 2: Non-transfected lysate.
AP13457PU-N - ZBTB7B
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZBTB7B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP13457PU-N - ZBTB7B
Western blot analysis of ZBTB7B Antibody (Center) Pab in mouse brain tissue lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab (1:60 dilution).
H00051043-B01P - ZBTB7B
Western Blot analysis of ZBTB7B expression in transfected 293T cell line (H00051043-T02) by ZBTB7B MaxPab polyclonal antibody.Lane 1: ZBTB7B transfected lysate(59.29 KDa).Lane 2: Non-transfected lysate.
AP09084SU-N - ZBTB7B
Western blot using anti-cKroxantibody to detect endogenous cKrox in mouse thymocyte whole cell lysates. The left lane is a control with no primary antibody. The center lane is a reagent control. The right lane shows a band at 80 kDa corresponding to mouse cKrox protein. The membrane was probed with the primary antibody at a 1:500 dilution. Personal Communication, L. Wang and R. Bossulet, National Cancer Institute, Bethesda, MD.
AP22983PU-N - ZBTB7B
Testis, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP22983PU-N - ZBTB7B
Adrenal, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM06405SU-N - ZBTB7B
Immunohistochemical analysis of paraffin-embedded human Breast tissues using anti-ZBTB7B antibody Cat.-No AM06405SU-N
AM06405SU-N - ZBTB7B
Immunofluorescence analysis of Hela cells using ZBTB7B antibody Cat.-No AM06405SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
AM06405SU-N - ZBTB7B
Western blot analysis using ZBTB7B antibody Cat.-No AM06405SU-N against HEK293 (Lanes 1,2) cell lysate.
TA331139 - ZBTB7B
WB Suggested Anti-ZFP67 Antibody Titration: 1.0-2.0ug/ml; Positive Control: HepG2 cell lysate
TA331139 - ZBTB7B
Host: Rabbit; Target Name: ZFP67; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0ug/ml
TA331139 - ZBTB7B
Host: Rabbit; Target Name:ZFP67; Sample Tissue:HepG2; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1.25ug/mL; Peptide Concentration: 1.0ug/mL; Lysate Quantity: 25ug/lane; Gel Concentration: 12%
TA331139 - ZBTB7B
Human Lung
TA331139 - ZBTB7B
Human Heart
TA352278 - ZBTB7B
Western blot analysis of Hela (lane 1), mouse thymus (lane 2), MCF-7 (lane 3) and THP-1 (lane 4) cells using anti-Th-POK (ZFP-67) antibody (clone 11H11A14). GAPDH antibody (poly6314) was used as loading control.
TA352278 - ZBTB7B
Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 ug/mL anti-Th-POK (ZFP-67) Antibody (11H11A14) and followed by DyLighttrade; 594 goat anti-mouse IgG (red) for 1 hour at room temperature. Actin filaments were labeled with Alexa Fluo488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZBTB7B antibody
Immunohistochemical analysis of paraffin-embedded human Breast tissues using anti-ZBTB7B antibody Cat.-No AM06405SU-N Mouse IgG1 7C12 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+2 additional images

ZBTB7B antibody
Western blot using anti-cKrox antibody to detect endogenous cKrox in mouse thymocyte whole cell lysates. The left lane is a control with no primary antibody. The center lane is a reagent control. The right lane shows a band at 80 kDa corresponding to mouse cKrox protein. The membrane was probed with the primary antibody at a 1:500 dilution. Personal Communication, L. Wang and R. Bossulet, National Cancer Institute, Bethesda, MD. Rabbit IgG Serum Hu, Ms, Rt E, WB
0.1 ml / €515.00
  OriGene Technologies GmbH

ZBTB7B (Center) antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZBTB7B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

ZBTB7B - human gene knockout kit via CRISPR
ZBTB7B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ZBTB7B - human gene knockout kit via CRISPR
ZBTB7B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

5 Item(s)

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Available Filters

Product Type
  1. CRISPR (2)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (2)
  2. Monoclonal (1)
Target
  1. ZBTB7B (3)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Ascites (1)
  2. Purified (1)
  3. Serum (1)
Host
  1. Rabbit (2)
  2. Mouse (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Species (CRISPR)
  1. Human (2)
Donor Vector
  1. GFP-Puro (1)
  2. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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