ZEB1 antibody
Principal name
ZEB1 antibody
Alternative names for ZEB1 antibody
AREB6, TCF8, TCF-8, NIL-2-A, Zinc finger E-box-binding homeobox 1, Negative regulator of IL2, Transcription factor 8, NIL-2-A zinc finger protein
Ncbi ID
9606, NP_001121600.1, NP_110378, NP_001121600, NM_011546, NM_030751, NM_001174095, NM_001174094, NM_001174096
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)
Background of ZEB1 antibody
ZEB1, also known as AREB6, is a zinc finger transcription factor encoding protein that represses T-lymphocyte-specific interleukin-2 (IL-2) gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (PMID: 1840704). ZEB1 is expressed in both skeletal and heart muscle, but is not found in the liver. ZEB1 mutations can cause posterior polymorphous corneal dystrophy type 3 (PPCD3) as well as corneal dystrophy Fuchs endothelial type 6 (FECD6). The former is a condition characterized by the metaplasia and overgrowth of corneal endothelial cells thus leading to an increased risk for glaucoma. The latter ocular disorder, FECD6, leads to reduced vision and pain and is caused by loss of endothelium of the central cornea. ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases.
General readings
Park SM, Gaur AB, Lengyel E, Peter ME.
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2.
Genes Dev. 2008 Apr 22 (7): 894-907.
PMID: 18381893
Human Fetal Muscle; Host:. Rabbit. Target Name:. AREB6. Sample Tissue:. Human Fetal Muscle cell lysates. Antibody Dilution: 1.0ug/ml; AREB6 antibody - N-terminal region (P100657_T100) in Human Fetal Muscle cells using Western Blot
ZEB1 polyclonal antibody ( Cat # PAB11610 ) ( 0.05 µg/ml ) staining of mouse heart lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Eye; . Sample Type : Eye tissue .; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human Eye cells using Immunofluorescence
Human HeLa; . Sample Type : Eye tissue .; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human HeLa cells using Immunofluorescence
Human Eye; . Sample Type : Eye tissue .; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human Eye cells using Immunofluorescence
Human Jurkat; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. ZEB1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human Jurkat cells using Western Blot
Human Capan1; . Sample Type :. Human Capan1 cells (Pancreatic cancer cell line). Primary Antibody Dilution :. 1:300. Secondary Antibody :. Anti-rabbit-AlexaFluor-488. Secondary Antibody Dilution :. 1:200. Color/Signal Descriptions :. ZEB: Green DAPI: Blue. Gene Name :. ZEB1. Submitted by :. Dr. Pankaj Singh, UNMC, Omaha, NE; ZEB1 antibody - N-terminal region (ARP30976_P050) in Human Capan1 cells using Immunohistochemistry
Human capan1, HPAF; . Lanes: 1. 45ug capan1 cell lysate . 2. 45 ug HPAF cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-Rabbit HRP. Secondary Antibody Dilution: 1:5000. Gene Name: ZEB1. Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE.; ZEB1 antibody - N-terminal region (ARP30976_P050) in Human capan1, HPAF cells using Western Blot
Human 293T; WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; ZEB1 antibody - N-terminal region (ARP30976_P050) in Human 293T cells using Western Blot
Formalin-Fixed, Paraffin-Embedded Human Heart stained with ZEB1 Antibody Cat.-No AP55676PU-N
Western Blot analysis of HEK293 cell lysates with anti-AREB6 Antibody Cat.-No AP55676PU-N at 1/100 dilution
Western Blot: TCF8 Antibody - Western blot detection of TCF8 in U2OS cell lysate.
ELISA: ZEB1 Antibody
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802295)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-ZEB1 monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Cross-reactivity test with ZEB2: HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right) and immunoblotted with anti-ZEB1 (TA802298).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZEB1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802309)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ZEB1 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
TA305688 (0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of HEK293 cell lysates with anti-AREB6 antibody (1:100).
Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1ug/mL.
Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 ug/mL.
Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 ug/mL.
Western blot analysis of MCF7 cell and PC-3 cell lysate using ZEB1 antibody.
Western blot analysis of extracts of various cell lines, using ZEB1 antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using ZEB1 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded mouse esophagus using ZEB1 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded rat lung using ZEB1 antibody at dilution of 1:100 (400x lens).
Immunofluorescence analysis of U20S cell using ZEB1 antibody. Blue: DAPI for nuclear staining.
Western Blot: ZEB1 Antibody [TA336897] - WB analysis of Zeb1 in 1) HeLa 2) Cos7 3) HepG2 and 4) Ntera2 cell lysates.
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [TA336897] - ZEB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: ZEB1 Antibody (2A8A6) [TA336943] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.
Immunohistochemistry: ZEB1 Antibody (2A8A6) [TA336943] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining.
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) [TA336943] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow Cytometry: ZEB1 Antibody (2A8A6) [TA336943] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA802298 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTIG6 (formerly G6) | |||||||
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Mouse | IgG2a | OTIG6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Top Selling product! Highly validated antibody!
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| TA332781 | ZEB1 antibody |
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| Rabbit anti-ZEB1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | C, ICC/IF, P, WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA802313 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI7E12 (formerly 7E12) | |||||||
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Mouse | IgG2a | OTI7E12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
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| TA305688 | ZEB1 (both Isoforms) antibody |
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| Goat Anti-AREB6 / ZEB1 Antibody | |||||||
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Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA310041 | ZEB1 antibody |
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| Rabbit anti-ZEB1 (AREB6) polyclonal antibody | |||||||
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Rabbit | Purified | Hu | WB |
0.1 ml /
€220.00
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| OriGene Technologies, Inc. | |||||||
| TA319713 | ZEB1 (Center) antibody |
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| Rabbit Polyclonal ZEB1 Antibody | |||||||
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Rabbit | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA332404 | ZEB1 antibody |
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| Rabbit anti-ZEB1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA336897 | ZEB1 antibody |
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| Rabbit Polyclonal ZEB1 Antibody | |||||||
![Western Blot: ZEB1 Antibody [TA336897] - WB analysis of Zeb1 in 1) HeLa 2) Cos7 3) HepG2 and 4) Ntera2 cell lysates.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-13159-1-W.jpg) |
Rabbit | Purified | Hu, Prim | ICC/IF, WB |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336943 | ZEB1 antibody |
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| Mouse Monoclonal ZEB1 Antibody (2A8A6) | |||||||
![Immunohistochemistry: ZEB1 Antibody (2A8A6) [TA336943] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-23484-1-H.jpg) |
Mouse | IgG1 | 2A8A6 | Purified | Hu | C, F, ICC/IF, P, WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA802295 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI8E2 (formerly 8E2) | |||||||
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Mouse | IgG2a | OTI8E2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA802302 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI8A2 (formerly 8A2) | |||||||
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Mouse | IgG2b | OTI8A2 | Purified | Hu | WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
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| TA802309 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI9A3 (formerly 9A3) | |||||||
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Mouse | IgG1 | OTI9A3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA802311 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8) | |||||||
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Mouse | IgG2a | OTI5B8 | Purified | Hu | WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
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| TA802321 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI10A12 (formerly 10A12) | |||||||
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Mouse | IgG2b | OTI10A12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA802336 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI8H8 (formerly 8H8) | |||||||
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Mouse | IgG1 | OTI8H8 | Purified | Hu | WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
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| TA802338 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI4B8 (formerly 4B8) | |||||||
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Mouse | IgG1 | OTI4B8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| TA802351 | ZEB1 antibody |
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| ZEB1 mouse monoclonal antibody, clone OTI9G3 (formerly 9G3) | |||||||
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Mouse | IgG2a | OTI9G3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AP55676PU-N | ZEB1 antibody |
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Rabbit | IgG | Purified | Hu | P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN217704 | ZEB1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN217704BN | ZEB1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||