ZHX2 antibody
Principal name
ZHX2 antibody
Alternative names for ZHX2 antibody
AFR1, RAF, KIAA0854, Zinc fingers and homeoboxes protein 2, Zinc finger and homeodomain protein 2, Alpha-fetoprotein regulator 1, AFP regulator 1, Regulator of AFP
Ncbi ID
9606, NP_055758, NP_955520, NP_055758.1, NM_014943, NP_955520.1, NM_199449
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of ZHX2 antibody
- Kit Component:
- - KN320081G1, Zhx2 gRNA vector 1 in pCas-Guide vector
- KN320081G2, Zhx2 gRNA vector 2 in pCas-Guide vector
- KN320081D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Perincheri,S., et al., (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (2), 396-401
Immunoperoxidase of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to ZHX2 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi ( Cat # H00022882-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody (M01), clone 5E2 (Cat # H00022882-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of ZHX2 expression in transfected 293T cell line by ZHX2 monoclonal antibody (M01), clone 5E2.Lane 1: ZHX2 transfected lysate(92.307 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-ZHX2 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ZHX2 antibody - N-terminal region (ARP39156_T100) in Human Jurkat cells using Western Blot
Mouse SP2/0; WB Suggested Anti-ZHX2 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: SP2/0 cell lysate; ZHX2 antibody - C-terminal region (ARP37629_T100) in Mouse SP2/0 cells using Western Blot
ZHX2 MaxPab polyclonal antibody. Western Blot analysis of ZHX2 expression in human placenta.
Western Blot analysis of ZHX2 expression in transfected 293T cell line (H00022882-T01) by ZHX2 MaxPab polyclonal antibody.Lane 1: ZHX2 transfected lysate(92.07 KDa).Lane 2: Non-transfected lysate.
ZHX2 MaxPab polyclonal antibody. Western Blot analysis of ZHX2 expression in human placenta.
Western Blot analysis of ZHX2 expression in transfected 293T cell line (H00022882-T01) by ZHX2 MaxPab polyclonal antibody.Lane 1: ZHX2 transfected lysate(92.07 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ZHX2 expression in transfected 293T cell line (H00022882-T02) by ZHX2 MaxPab polyclonal antibody.Lane 1: ZHX2 transfected lysate(92.30 KDa).Lane 2: Non-transfected lysate.
ZHX2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ZHX2 expression in Hela NE.
Immunohistochemical analysis of ZHX2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ZHX2 expression in HepG2 (A); K562 (B) whole cell lysates.
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308030 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2(TA308030) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZHX2(TA308030) antibody at 1:200 dilution.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ZHX2 antibody.The picture on the right is treated with the synthesized peptide.
ZHX2 Antibody (N-term)(Cat. #TA324654) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).
IF image of A549 cell stained with ZHX2 Antibody (N-term)(Cat#TA324654).A549 cells were incubated with ZHX2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
WB Suggested Anti-ZHX2 Antibody Titration: 2.5ug/ml; ELISA Titer: 1:62500; Positive Control: SP2/0 cell lysate
WB Suggested Anti-ZHX2 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308030 | ZHX2 antibody |
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| Rabbit polyclonal antibody to ZHX2 (zinc fingers and homeoboxes 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA312720 | ZHX2 antibody |
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| Rabbit polyclonal anti-ZHX2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324654 | ZHX2 antibody |
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| Rabbit polyclonal ZHX2 Antibody (N-term) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308321 | ZHX2 |
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| Recombinant protein of human zinc fingers and homeoboxes 2 (ZHX2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414909 | ZHX2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210894L2V | Lenti ORF particles, Zhx2 (GFP-tagged) - Mouse zinc fingers and homeoboxes 2 (Zhx2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,660.00
|
|
| OriGene Technologies, Inc. | ||
| MR210894L1V | Lenti ORF particles, Zhx2 (Myc-DDK-tagged) - Mouse zinc fingers and homeoboxes 2 (Zhx2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,490.00
|
|
| OriGene Technologies, Inc. | ||
