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ZIC2 antibody

Principal name

ZIC2 antibody

Alternative names for ZIC2 antibody

HPE5, Zinc finger protein ZIC 2, Zinc finger protein of the cerebellum 2

SwissProt ID

O95409 (Human), Q62520 (Mouse), Q91689

Gene ID

7546 (ZIC2), 22772 (Zic2)

Ncbi ID

9606, NP_009060, 10090, NP_009060.2, NM_009574

Available reactivities

Hu (Human), Chimp (Chimpanzee), Chk (Chicken), Frog, Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ZIC2 antibody

ZIC2 is a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects.This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13.

General readings

1. Dubourg,C., et al. (2004) Molecular screening of SHH, ZIC2, SIX3, and TGIF genes in patients with features of holoprosencephaly spectrum: Mutation review and genotype-phenotype correlations. Hum. Mutat. 24 (1), 43-51.
2. Herrera,E., Brown,L., Aruga,J., Rachel,R.A., Dolen,G., Mikoshiba,K., Brown,S. and Mason,C.A. (2003) Zic2 patterns binocular vision by specifying the uncrossed retinal projection. Cell 114 (5), 545-557.
3. Brown,L.Y., Hodge,S.E., Johnson,W.G., Guy,S.G., Nye,J.S. and Brown,S. (2002) Possible association of NTDs with a polyhistidine tract polymorphism in the ZIC2 gene. Am. J. Med. Genet. 108 (2), 128-131.
Marcorelles,P., Loget,P., Fallet-Bianco,C., Roume,J., Encha-4. Razavi,F. and Delezoide,A.L. (2002) Unusual variant of holoprosencephaly in monosomy 13q. Am. J. Med. Genet. 5 (2), 170-178.
5. Yang,Y., Hwang,C.K., Junn,E., Lee,G. and Mouradian,M.M. (2000) ZIC2 and Sp3 repress Sp1-induced activation of the human D1A dopamine receptor gene. J. Biol. Chem. 275 (49), 38863-38869.
6. Brown,S.A., Warburton,D., Brown,L.Y., Yu,C.Y., Roeder,E.R., Stengel-Rutkowski,S., Hennekam,R.C. and Muenke,M. (1998) Holoprosencephaly due to mutations in ZIC2, a homologue of Drosophila odd-paired. Nat. Genet. 20 (2), 180-183.

Human Skin; ZIC2 antibody - C-terminal region (ARP35821_P050) in Human Skin cells using Immunohistochemistry

ARP35821_P050

Human HepG2; WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ZIC2 antibody - C-terminal region (ARP35821_P050) in Human HepG2 cells using Western Blot

ARP35821_P050

Western blot using Zic2 polyclonal antibody ( Cat # PAB11336 ) shows detection of a band ~55 kDa ( arrowhead ) corresponding to Zic2 in lysates from ( lane1 ) mouse brain and ( lane 2 ) rat brain.Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Zic2 polyclonal antibody ( Cat # PAB11336 ).The upper band in the mouse brain lysate represents Zic2.

PAB11336

TP320798

RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with TA311376 (0.5ug/ml) in the right panel and probed with anti-MYC Tag (1:1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in Lanes 1 and expressing GFP in Lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.

TA311376

Western blot using Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.

TA319447

WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate

TA339144

Human Skin

TA339144

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA311376	ZIC2 (C-term) antibody
	Goat Anti-ZIC2 Antibody
	Goat		Aff - Purified	Bov, Can, Hu, Ms, Rt	E, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA319447	ZIC2 (189-206) antibody
	Rabbit polyclonal anti-ZIC-2 antibody
	Rabbit	IgG	Aff - Purified	Can, Chimp, Hu, Ms, Rt	E, WB	0.1 mg / €515.00
						OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP320798	ZIC2
	Recombinant protein of human Zic family member 2 (odd-paired homolog, Drosophila) (ZIC2)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

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