ZNF148 antibody
Principal name
ZNF148 antibody
Alternative names for ZNF148 antibody
ZBP89, Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89
Ncbi ID
NP_068799.2, 9606, NP_068799, NM_021964, NP_035879.1, NM_031615, NM_011749
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of ZNF148 antibody
ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.
Figure 1. Serum starvation induces ZBP-89 and p53expression. AGS (gastric carcinoma) cells were cultured in serum-free F-12 medium for the indicated times, and immunoblots were used to detect the expression profiles of ZBP-89, p53, and p14ARF. Blotting was with Rabbit-anti-ZBP-89 antibody. For detection use HRP conjugated Gt-anti-Rabbit IgG. See Bai and Merchant (2001) for additional details.
Figure 2. Anti-ZBP-89 antibody used to confirm siRNA knockdown of ZBP-89. SeeBai and Merchant (2003) for additional details.
Human Placenta; WB Suggested Anti-ZNF148 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Placenta; ZNF148 antibody - middle region (ARP34536_P050) in Human Placenta cells using Western Blot
Human Lung; . Rabbit Anti-ZNF148 Antibody. . Catalog Number:. ARP34606 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human OVCAR3; WB Suggested Anti-ZNF148 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate. ZNF148 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells.; ZNF148 antibody - middle region (ARP34606_P050) in Human OVCAR3 cells using Western Blot
Immunohistochemistry (IHC) analyzes of ZBP-89 antibody (Cat.-No.: AP20538PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of ZBP-89 antibody (Cat.-No.: AP20538PU-N) in extracts from HepG2 cells.
Immunohistochemical analysis of ZNF148 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ZNF148 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ZNF148 expression in HeLa (A); HepG2 (B) whole cell lysates.
Western blot analysis of extracts from HepG2 cells, using ZNF148 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZNF148 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-ZNF148 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Placenta
WB Suggested Anti-ZNF148 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysateZNF148 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells
Rabbit Anti-ZNF148 Antibody; Paraffin Embedded Tissue: Human alveolar cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Western blot analysis on HepG2 cell lysate using ZNF148 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314693 | ZNF148 antibody |
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| Rabbit polyclonal anti-ZNF148 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA347789 | ZNF148 antibody |
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| Rabbit Polyclonal Anti-ZNF148 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP20538PU-N | ZNF148 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| R1493 | ZNF148 antibody |
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 |
Rabbit | Serum | Hu | E, WB |
0.1 ml /
€515.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322687 | ZNF148 |
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| Purified recombinant protein of Homo sapiens zinc finger protein 148 (ZNF148) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402892 | ZNF148 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210700L2V | Lenti ORF particles, Zfp148 (GFP-tagged) - Mouse zinc finger protein 148 (Zfp148), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,590.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214797L2V | Lenti ORF particles, Zfp148 (GFP-tagged ORF) - Rat zinc finger protein 148 (Zfp148), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,600.00
|
|
| OriGene Technologies, Inc. | ||
| RR214797L1V | Lenti ORF particles, Zfp148 (Myc-DDK-tagged ORF) - Rat zinc finger protein 148 (Zfp148), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,440.00
|
|
| OriGene Technologies, Inc. | ||