ZNF35 antibody
Principal name
ZNF35 antibody
Alternative names for ZNF35 antibody
Zinc finger protein 35
SwissProt ID
P13682 (Human)
Gene ID
7584 (ZNF35)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of ZNF35 antibody
- Kit Component:
- - KN203770G1, ZNF35 gRNA vector 1 in pCas-Guide vector
- KN203770G2, ZNF35 gRNA vector 2 in pCas-Guide vector
- KN203770D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Calabro,V., et al., (1995) Hum. Genet. 95 (1), 18-21
Human Jurkat; WB Suggested Anti-ZNF35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ZNF35 antibody - N-terminal region (ARP37722_P050) in Human Jurkat cells using Western Blot
Immunofluorescence of purified MaxPab antibody to ZNF35 on HeLa cell. [antibody concentration 10 ug/ml]
ZNF35 MaxPab polyclonal antibody. Western Blot analysis of ZNF35 expression in human ovary cancer.
Western Blot analysis of ZNF35 expression in transfected 293T cell line (H00007584-T01) by ZNF35 MaxPab polyclonal antibody.Lane 1: ZNF35 transfected lysate(57.2 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to ZNF35 on HeLa cell. [antibody concentration 10 ug/ml]
ZNF35 MaxPab polyclonal antibody. Western Blot analysis of ZNF35 expression in human ovary cancer.
Western Blot analysis of ZNF35 expression in transfected 293T cell line (H00007584-T01) by ZNF35 MaxPab polyclonal antibody.Lane 1: ZNF35 transfected lysate(57.2 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ZNF35 expression in transfected 293T cell line by ZNF35 monoclonal antibody (M03), clone 2B4.Lane 1: ZNF35 transfected lysate(58.2 KDa).Lane 2: Non-transfected lysate.
ZNF35 Antibody (Center) (AP54692PU-N) western blot analysis in Hela cell line lysates (35 µg/lane). This demonstrates the ZNF35 antibody detected the ZNF35 protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.(1:2000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ZNF35 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807032)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZNF35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807032)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ZNF35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807123)(1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ZNF35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807123)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ZNF35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807143)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ZNF35 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807143)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF35 (RC203770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF35.
WB Suggested Anti-ZNF35 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760552 | ZNF35 (N-term HIS tag) |
||||
| Purified recombinant protein of Human zinc finger protein 35 (ZNF35), with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
