ZNF449 antibody
Principal name
ZNF449 antibody
Alternative names for ZNF449 antibody
Zinc finger protein 449, ZSCAN19, Zinc finger and SCAN domain-containing protein 19
SwissProt ID
Q6P9G9 (Human)
Gene ID
203523 (ZNF449)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of ZNF449 antibody
- Kit Component:
- - KN209172G1, ZNF449 gRNA vector 1 in pCas-Guide vector
- KN209172G2, ZNF449 gRNA vector 2 in pCas-Guide vector
- KN209172D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Luo,K., (2006) Mol. Biol. Rep. 33 (1), 51-57
Immunofluorescence of purified MaxPab antibody to ZNF449 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ZNF449 expression in transfected 293T cell line (H00203523-T01) by ZNF449 MaxPab polyclonal antibody.Lane 1: ZNF449 transfected lysate(56.98 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to ZNF449 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ZNF449 expression in transfected 293T cell line (H00203523-T01) by ZNF449 MaxPab polyclonal antibody.Lane 1: ZNF449 transfected lysate(56.98 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; ZNF449 antibody - middle region (ARP31647_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; ZNF449 antibody - C-terminal region (ARP32254_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; ZNF449 antibody - N-terminal region (ARP32994_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; ZNF449 antibody - middle region (AP42048PU-N) in Transfected 293T cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF449 (RC209172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF449.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808530)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808530)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ZNF449 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808530)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF449 (RC209172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF449.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF449 (RC209172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF449.(1:2000)
WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T
WB Suggested Anti-ZNF449 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808530 | ZNF449 antibody |
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| ZNF449 mouse monoclonal antibody, clone OTI4C6 (formerly 4C6) | |||||||
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Mouse | IgG1 | OTI4C6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA808529 | ZNF449 antibody |
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| ZNF449 mouse monoclonal antibody, clone OTI12E10 (formerly 12E10) | |||||||
 |
Mouse | IgG1 | OTI12E10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808599 | ZNF449 antibody |
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| ZNF449 mouse monoclonal antibody, clone OTI2C5 (formerly 2C5) | |||||||
 |
Mouse | IgG2a | OTI2C5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760199 | ZNF449 (full length, N-term HIS tag) |
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| Recombinant protein of human zinc finger protein 449 (ZNF449), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||