ZNF682 antibody
Principal name
ZNF682 antibody
Alternative names for ZNF682 antibody
Zinc finger protein 682
SwissProt ID
O95780 (Human)
Gene ID
91120 (ZNF682)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ZNF682 antibody
- Kit Component:
- - KN214494G1, ZNF682 gRNA vector 1 in pCas-Guide vector
- KN214494G2, ZNF682 gRNA vector 2 in pCas-Guide vector
- KN214494D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Isogai,T. Unpublished (1999)
Human Jurkat; WB Suggested Anti-ZNF682 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate; ZNF682 antibody - N-terminal region (ARP39750_T100) in Human Jurkat cells using Western Blot
Human Lung; WB Suggested Anti-ZNF682 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Lung; ZNF682 antibody - middle region (ARP39751_P050) in Human Lung cells using Western Blot
Immunofluorescent analysis of ZNF682 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ZNF682 expression in HeLa (A); Jurkat (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
Western blot analysis of extracts from COLO205 cells and HUVEC cells, using ZNF682 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-ZNF682 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 12500; Positive Control: Jurkat cell lysate
WB Suggested Anti-ZNF682 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Lung
Western blot analysis on COLO205 cell lysate using ZNF682 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315976 | ZNF682 antibody |
||||||
| Rabbit polyclonal anti-ZNF682 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347660 | ZNF682 antibody |
||||||
| Rabbit Polyclonal Anti-ZNF682 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409674 | ZNF682 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |