ZNRF1 antibody
Principal name
ZNRF1 antibody
Alternative names for ZNRF1 antibody
NIN283, E3 ubiquitin-protein ligase ZNRF1, Zinc/RING finger protein 1, Nerve injury-induced gene 283 protein
Ncbi ID
9606, NP_115644, NP_001162092.1, NM_032268, NP_115644.1, NM_001168621, NM_133206, NM_001168623
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of ZNRF1 antibody
- Kit Component:
- - KN203521G1, ZNRF1 gRNA vector 1 in pCas-Guide vector
- KN203521G2, ZNRF1 gRNA vector 2 in pCas-Guide vector
- KN203521D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to ZNRF1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Western Blot analysis of ZNRF1 expression in transfected 293T cell line (H00084937-T01) by ZNRF1 MaxPab polyclonal antibody.Lane1:ZNRF1 transfected lysate(24.97 KDa).Lane2:Non-transfected lysate.
Western Blot analysis of ZNRF1 expression in transfected 293T cell line (H00084937-T01) by ZNRF1 MaxPab polyclonal antibody.Lane1:ZNRF1 transfected lysate(24.97 KDa).Lane2:Non-transfected lysate.
Human Spleen; WB Suggested Anti-ZNRF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; ZNRF1 antibody - N-terminal region (ARP39690_P050) in Human Spleen cells using Western Blot
ZNRF1 Antibody (Center) (AP54736PU-N) western blot analysis in HepG2 cell line lysates (35 µg/lane). This demonstrates the ZNRF1 antibody detected the ZNRF1 protein (arrow).
ZNRF1 Antibody Cat.-No AP32741PU-N (10µg/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after Biotinylated anti-Goat secondary.
N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with ZNRF1 Antibody Cat.-No AP32741PU-N (1µg/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 24 kDa. Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
WB Suggested Anti-ZNRF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32741PU-N | ZNRF1 antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | P, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA322144 | ZNRF1 antibody |
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| Anti-ZNRF1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| AP54736PU-N | ZNRF1 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410254 | ZNRF1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |