ZSCAN4 antibody
Principal name
ZSCAN4 antibody
Alternative names for ZSCAN4 antibody
ZNF494, Zinc finger protein 494, Zinc finger and SCAN domain-containing protein 4
SwissProt ID
Q8NAM6 (Human)
Gene ID
201516 (ZSCAN4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ZSCAN4 antibody
Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.
ZSCAN4 MaxPab polyclonal antibody. Western Blot analysis of ZSCAN4 expression in human kidney.
Western Blot analysis of ZSCAN4 expression in transfected 293T cell line (H00201516-T01) by ZSCAN4 MaxPab polyclonal antibody.Lane 1: ZSCAN4 transfected lysate(47.63 KDa).Lane 2: Non-transfected lysate.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZSCAN4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ZSCAN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800536)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ZSCAN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800536)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4.
Western blot analysis of ZSCAN4 in mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of peptide blocking.
Immunohistochemistry of ZSCAN4 in rat lung tissue with ZSCAN4 antibody at 5 ug/mL.
Immunofluorescence of ZSCAN4 in Rat Lung tissue with ZSCAN4 antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306927 | ZSCAN4 antibody |
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| Rabbit Polyclonal ZSCAN4 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA800425 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI1E7 (formerly 1E7) | |||||||
 |
Mouse | IgG1 | OTI1E7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800431 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI3D1 (formerly 3D1) | |||||||
 |
Mouse | IgG1 | OTI3D1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800448 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI3B1 (formerly 3B1) | |||||||
 |
Mouse | IgG2a | OTI3B1 | Purified | Can, Hu | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA800535 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI2C5 (formerly 2C5) | |||||||
 |
Mouse | IgG1 | OTI2C5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA800536 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) | |||||||
 |
Mouse | IgG1 | OTI1A6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA800537 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
 |
Mouse | IgG2a | OTI1C6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA800539 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI5E8 (formerly 5E8) | |||||||
 |
Mouse | IgG1 | OTI5E8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA800540 | ZSCAN4 antibody |
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| ZSCAN4 mouse monoclonal antibody, clone OTI5A5 (formerly 5A5) | |||||||
 |
Mouse | IgG1 | OTI5A5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP23939PU-N | ZSCAN4 (C-term) antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311003 | ZSCAN4 |
||||
| Recombinant protein of human zinc finger and SCAN domain containing 4 (ZSCAN4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY407355 | ZSCAN4 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |