ZWILCH antibody
Principal name
ZWILCH antibody
Alternative names for ZWILCH antibody
hZwilch, Protein zwilch homolog
Ncbi ID
9606, NP_060445.3, NP_060445, NM_017975, NP_080783.3, BC027435
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of ZWILCH antibody
- Kit Component:
- - KN320172G1, Zwilch gRNA vector 1 in pCas-Guide vector
- KN320172G2, Zwilch gRNA vector 2 in pCas-Guide vector
- KN320172D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wang,C., et al. (2008) . Biol. Chem. 283 (17), 11565-11574
Meng,F., et al. (2003) Brain Res. 967 (1-2), 161-169
Western Blot analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.Lane 1: ZWILCH transfected lysate(54.224 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ZWILCH is approximately 1ng/ml as a capture antibody.
Western Blot analysis of ZWILCH expression in transfected 293T cell line (H00055055-T01) by ZWILCH MaxPab polyclonal antibody.Lane 1: ZWILCH transfected lysate(52.47 KDa).Lane 2: Non-transfected lysate.
Western blot analysis in mouse spleen tissue lysates (35ug/lane) using ZWILCH Antibody (C-term) (Cat.-No AP33477PU-N). This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).
Western blot analysis in 293 cell line lysates (35ug/lane) using ZWILCH Antibody (C-term) (Cat.-No AP33477PU-N). This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow).
Predicted band size: 67 kDa. Positive control: HT-29 and hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: HT-29 cell lysate Lane2: HT-29 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA322300 | ZWILCH antibody |
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| Anti-ZWILCH Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP33477PU-N | ZWILCH (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY413408 | ZWILCH overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209182L2V | Lenti ORF particles, Zwilch (GFP-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (cDNA clone MGC:35649 IMAGE:2812472), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR209182L1V | Lenti ORF particles, Zwilch (Myc-DDK-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (cDNA clone MGC:35649 IMAGE:2812472), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
