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AP09280PU-N CDC27 (422-430) antibody

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0.1 mg / €515.00
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Quick Overview

Rabbit anti Canine, Chimpanzee, Chicken, Human, Rat CDC27

AP09280PU-N

Product Description for CDC27

Rabbit anti Canine, Chimpanzee, Chicken, Human, Rat CDC27.
Properties: (422-430)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for CDC27

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC
Presentation Aff - Purified
Reactivity Can, Chimp, Chk, Hu, Rt
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P30260
Immunogen:
Synthetic peptide corresponding amino acids 422-430 of Human cdc27
GeneID:
996
Property 422-430
Application ELISA: 1/5,000 - 1/40,000.
Western Blot: 1/500 - 1/2,000.
Background Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Concentration 1.95 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Gabellini D, Colaluca IN, Vodermaier HC, Biamonti G, Giacca M, Falaschi A, et al. Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J. 2003 Jul 15;22(14):3715-24. PubMed PMID: 12853486. (Free PMC Article available, 7 images available)
  2. Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002 Jul-Aug;1(4):282-92. PubMed PMID: 12429948.
  3. Wassmann K, Benezra R. Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11193-8. PubMed PMID: 9736712. (Free PMC Article available, 5 images available)
  4. Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science. 1998 Feb 20;279(5354):1219-22. PubMed PMID: 9469815.
  5. Ho PP, Couch FJ, Brody LC, Abel KJ, Boehnke M, Shearon TH, et al. Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3. Somat Cell Mol Genet. 1995 Sep;21(5):351-5. PubMed PMID: 8619132.
  6. Tugendreich S, Tomkiel J, Earnshaw W, Hieter P. CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell. 1995 Apr 21;81(2):261-8. PubMed PMID: 7736578.
  7. Chen PL, Ueng YC, Durfee T, Chen KC, Yang-Feng T, Lee WH. Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner. Cell Growth Differ. 1995 Feb;6(2):199-210. PubMed PMID: 7756179.
  8. Tugendreich S, Boguski MS, Seldin MS, Hieter P. Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10031-5. PubMed PMID: 8234252. (Free PMC Article available)
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State:
Liquid purified Ig
Aff - Purified
Species Reactivity
Species reactivity (expected):
Rat, Canine, Chicken, Chimpanzee, Mouse.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is directed against CDC27 protein.
Gene ID 996

Accessory Products

Proteins and/or Positive Controls

Positive controls for CDC27 (3 products)

Catalog No. Species Pres. Purity   Source  

Cdc27 Lysate
Western Blot: Cdc27 Lysate [NBL1-08998] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CDC27
  Novus Biologicals Inc.

CDC27 overexpression lysate
CDC27 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

CDC27 overexpression lysate
CDC27 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for CDC27 (9 products)

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Rabbit IgG (H+L chain) antibody
Rabbit IgG (H+L chain) antibody Goat TRITC Rb E, F, ICC/IF
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Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody Donkey PE Rb F
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Rabbit IgG (H+L chain, adsorbed) antibody
Rabbit IgG (H+L chain, adsorbed) antibody Goat Cy2
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Rabbit IgG (H+L chain, adsorbed) antibody
Rabbit IgG (H+L chain, adsorbed) antibody Goat Cy3
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Rabbit IgG (H+L chain, adsorbed) antibody
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Click here to see all secondary antibodies for CDC27
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