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CDC27 antibody

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Principal name

CDC27 antibody

Alternative names for CDC27 antibody

CDC27Hs, H-NUC, D0S1430E, D17S978E, Cell division cycle protein 27 homolog

SwissProt ID

P30260 (Human)

Gene ID

996 (CDC27)

Ncbi ID

NP_001107563, 9606, NP_001107563.1, NP_001247, ABM87026.1, NM_001256, NM_001114091

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken), Bov (Bovine), Can (Canine), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CDC27 antibody

APC3 (anaphase-promoting complex subunit 3) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 95 kD. The APC3 protein is located at the spindle poles, the spindle microtubules, kinetochores and chromosome arms. This protein is required for core stability and functions as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC3 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC3 protein interacts with Rb, Mad2, p55CDC, BUBR1, as well as the APC complex proteins noted above. The APC3 protein can be modified by phosphorylation by cdk1-cyclin B.

General readings

1. Koloteva-Levine, N., et al., Mol. Cell. Biol. 24, 3577-3587 (2004)
2. Laronne, A., et al., Mol. Biol. Cell 14, 3730-3740 (2003)
3. Zur, A., et al., EMBO J. 21, 4500-4510 (2002)
4. Yamanaka, A., et al., Mol. Biol. Cell 11, 2821-2831 (2000)
5. Yamano, H., et al., EMBO J. 17, 5670-5678 (1998)
Clone AF3.1 is used in these references.

AP06780PU-N - CDC27
Western blot (WB) analysis of Cdc27 antibody (Cat.-No.: AP06780PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:HEK293T whole cell lysate
Lane3:Rat kidney tissue lysate
PAB9986 - CDC27
Western blot using CDC27 polyclonal antibody ( Cat # PAB9986 ) shows detection of a band ~90 kDa corresponding to human CDC27 ( arrowhead ).Approximately 35 µg of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h atroom temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developedby LI-COR.IRDye is a trademark of LI-COR, Inc.
PAB9988 - CDC27
Western blot using CDC27 (phospho T244) polyclonal antibody ( Cat # PAB9988 ) shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 ( arrowhead ).Lane 1 shows lysate from asynchronous cells.Lane 2 shows lysate from cells treated with nocodazole.Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.Each lane contains approximately 30 µg of HeLa whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.ECL reagent was used for detection.Data contributed by Bing Li, UT South western.
ARP43603_P050 - CDC27
Human NCI-H226; WB Suggested Anti-CDC27 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NCI-H226 cell lysate.. CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226.; CDC27 antibody - middle region (ARP43603_P050) in Human NCI-H226 cells using Western Blot
AM20198AF-N - CDC27
Western blot anaylsis using AM20198AF-N CDC27 Antibody: CDC27 expression in Jurkat (Lane 1), Raji (Lane 2), K562 (Lane 3), HL-60 (Lane 4) and MCF-7 (Lane 5) cells.
AM20198AF-N - CDC27
Immunoprecipitation of CDC27 from Raji cells with AM20198AF-N (Lane 1) or normal Mouse IgG (Lane 2). After Immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and mmunoblotted with AM20198AF-N CDC27 antibody.
C12168-1 - CDC27
Western blot analysis of extracts from HuvEc/HepG2/COLO205 cells, using H-NUC Antibody. The lane on the right is treated with the synthesized peptide.
AP23516PU-N - CDC27
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23516PU-N - CDC27
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23516PU-N - CDC27
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 µg/ml.
AM06470SU-N - CDC27
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 antibody Cat.-No AM06470SU-N with DAB staining.
AM06470SU-N - CDC27
Western blot analysis using CDC27 antibody Cat.-No AM06470SU-N against CDC27 (AA: 724-830)-hIgGFc transfected HEK293 cell.
CPA1195-100ul - CDC27
Immunohistochemical analysis of CDC27 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1195-100ul - CDC27
Western blot analysis of CDC27 expression in A549 (A); K562 (B); Jurkat (C); HEK293T (D); HeLa (E) whole cell lysates.
TA313394 - CDC27
Western blot analysis of extracts from HUVEC cells, HepG2 cells and COLO205 cells, using H-NUC antibody.The lane on the right is treated with the synthesized peptide.
TA319297 - CDC27
WB using Anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.The primary antibody was diluted to 1:500. HRP Gt-a-Rabbit IgG [H&L] MX was used at 1:5000.
TA327926 - CDC27
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against APC3. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
TA327926 - CDC27
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against APC3, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
TA332147 - CDC27
WB Suggested Anti-CDC27 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: NCI-H226 cell lysate.CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226
TA342554 - CDC27
Host: Rabbit; Target Name: CDC27; Sample Tissue: Fetal Liver lysates; Antibody Dilution: 1.0 ug/ml
TA349015 - CDC27
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/mL.
TA349015 - CDC27
Immunohistochemistry of APC3 in rat liver tissue with APC3 antibody at 5 ug/mL.
TA351061 - CDC27
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: HepG2 cells, K562 cells, Primary antibody: (CDC27 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
TA351061 - CDC27
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (CDC27 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC27 antibody
  Rabbit polyclonal anti-H-NUC antibody  
Western blot analysis of extracts from HUVEC cells, HepG2 cells and COLO205 cells, using H-NUC antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CDC27 pThr244 antibody
  Rabbit polyclonal CDC27 phospho T244 antibody  
WB using Anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.The primary antibody was diluted to 1:500. HRP Gt-a-Rabbit IgG [H&L] MX was used at 1:5000. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Rt E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

CDC27 (N-term) antibody
  Rabbit Polyclonal anti-APC3 Antibody  
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against APC3. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu ICC/IF, WB
50 µl / €130.00
  OriGene Technologies, Inc.
+1 additional image

CDC27 antibody
  Rabbit Polyclonal Anti-APC3 Antibody  
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

CDC27 antibody
  Rabbit Polyclonal Anti-CDC27 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: HepG2 cells, K562 cells, Primary antibody: (CDC27 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

CDC27 antibody
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 antibody Cat.-No AM06470SU-N with DAB staining. Mouse IgG1 5C12 Ascites Hu E, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

CDC27 (C-term) antibody
Western blot anaylsis using AM20198AF-N CDC27 Antibody: CDC27 expression in Jurkat (Lane 1), Raji (Lane 2), K562 (Lane 3), HL-60 (Lane 4) and MCF-7 (Lane 5) cells. Mouse IgG2b AF3.1 Azide Free Bov, Can, Hu, Ms, Rt, Xen E, IP, P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+1 additional image

CDC27 antibody
Western blot (WB) analysis of Cdc27 antibody (Cat.-No.: AP06780PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:HEK293T whole cell lysate Lane3:Rat kidney tissue lysate Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

CDC27 pSer427 antibody
CDC27 Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Rt E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

CDC27 (422-430) antibody
CDC27 Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Rt E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

CDC27 (C-term) antibody
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

Lysates

Catalog No.    

CDC27 overexpression lysate
CDC27 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

CDC27 overexpression lysate
CDC27 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

13 Item(s)

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  1. Polyclonal (9)
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Property
  1. 422-430 (1)
  2. C-term (2)
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Reactivity
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Applications
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  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
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