Product Description for KEAP1
Rabbit anti Bovine, Human, Mouse, Porcine, Rat, African clawed frog KEAP1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.
Properties for KEAP1
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 50 µg |
| Synonyms | Cytosolic inhibitor of Nrf2, INRF2, INrf2, KEAP1, KIAA0132, KLHL19, Kelch-like ECH-associated protein 1, Kelch-like protein 19 |
| Presentation | Aff - Purified |
| Reactivity | African clawed frog, Bov, Hu, Ms, Por, Rt |
| Applications | P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Europe only |
| PDF datasheet | View Datasheet |
| Manufacturer | AVIVA Systems Biology |
Datasheet Extract
| Immunogen |
|
|---|---|
| Add. information | Rabbit Ig G is purified by peptide affinity chromatography method. 70kDa, 70kDa |
| Application | Suggested dilution at 0.5 ELISA titre using peptide based assay: 1:12500 |
| Background | KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
| General Readings | Padmanabhan,B., (2006) Mol. Cell 21 (5), 689-700 |
| Storage | Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20DegC. Avoid repeat freeze-thaw cycles. |
| Format |
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| Specificity |
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| Gene ID | 9817 |
Accessory Products
Proteins and/or Positive Controls
Proteins for KEAP1 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP302189 |
KEAP1 (transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP302513 |
KEAP1 (transcript variant 2) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| H00009817-P01-10 |
KEAP1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00009817-P01-25 |
KEAP1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for KEAP1 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-12225 |
KEAP1 Lysate |
||||||
![Western Blot: KEAP1 Lysate [NBL1-12225] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KEAP1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/KEAP1%20Lysate-Western%20Blot-NBL1-12225-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
| NBL1-12226 |
KEAP1 Lysate |
||||||
![Western Blot: KEAP1 Lysate [NBL1-12226] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KEAP1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/KEAP1%20Lysate-Western%20Blot-NBL1-12226-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for KEAP1 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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