KEAP1 antibody
Principal name
KEAP1 antibody
Alternative names for KEAP1 antibody
KEAP1, INRF2, KIAA0132, KLHL19, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19
Ncbi ID
NP_987096, 9606, NP_036421, NP_036421.2, NP_001103775.1, NM_001110307, NM_016679, NM_001110305, NM_001110306, NM_057152, NM_203500, NM_012289
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of KEAP1 antibody
The nuclear factor-like 2 (NRF2) transcriptiol activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation. Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes. The coordited action of NRF2 and other transcription factors mediates the response to oxidative stress. Altered expression of NRF2 is associated with chronic obstructive pulmory disease (COPD). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siR enhances anti-cancer drug-induced apoptosis.The NRF2 repressor KEAP1 contains an amino termil BTB/POZ domain and a carboxyl termil KELCH domain. The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase. Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-?B activity by targeting IKK¦Â degradation. Mutation of the corresponding KEAP1 gene is seen in lung cancer cases and can lead to uncontrolled activation of NRF2.
General readings
Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53.
Human Muscle; KEAP1 antibody - C-terminal region (ARP38981_P050) in Human Muscle cells using Immunohistochemistry
Transfected 293T; WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Transfected 293T; KEAP1 antibody - C-terminal region (ARP38981_P050) in Transfected 293T cells using Western Blot
Human Kidney; Kidney; KEAP1 antibody - N-terminal region (ARP34727_P050) in Human Kidney cells using Immunohistochemistry
Transfected 293T; WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; KEAP1 antibody - N-terminal region (ARP34727_P050) in Transfected 293T cells using Western Blot
Human kidney; KEAP1 antibody - C-terminal region (ARP34728_T100) in Human kidney cells using Immunohistochemistry
HepG2; Host: Rabbit. Target Name:KEAP1. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1.25ug/mL. Peptide Concentration: 1.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%. KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2.; KEAP1 antibody - C-terminal region (ARP34728_T100) in HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-KEAP1 Antibody Titration: 1.0ug/ml. Positive Control: HepG2 cell lysate. KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2.; KEAP1 antibody - C-terminal region (ARP34728_T100) in Human HepG2 cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle reacted with KEAP1 Antibody (C-term) Cat.-No AP52328PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of KEAP1 Antibody (C-term) Cat.-No AP52328PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).
AP32137PU-N KEAP1 antibody staining of paraffin embedded Human Cerebellum at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AP32137PU-N KEAP1 antibody staining of NIH3T3 lysate at 0.2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-KEAP1 mouse monoclonal antibody (TA501989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1(RC202189).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA501989), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA502056), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
Figure from citation: Western Blot of Keap1 protein level by using anti-Keap1 antibody in human BEAS2B cells. View Citation
HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA502059), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-KEAP1 Rabbit Polyclonal antibody. (TA590238)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202513, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
ELISA: KEAP1 Antibody
Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 ug/mL.
Immunofluorescence of KEAP1 in human lung tissue with KEAP1 antibody at 20 ug/mL.
Predicted band size: 70 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of HepG2 cell lines, using KEAP1 antibody.
WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
Kidney
WB Suggested Anti-KEAP1 Antibody Titration: 1.0 ug/ml; Positive Control: HepG2 cell lysate KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2
Host: Rabbit; Target Name:KEAP1; Sample Tissue:HepG2; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1.25ug/mL; Peptide Concentration: 1.0ug/mL; Lysate Quantity: 25ug/lane; Gel Concentration: 12%KEAP
Human kidney
WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Transfected 293T
Human Muscle
Western blot analysis of Keap1 expression in NIH 3T3 lysate
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (KEAP1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (KEAP1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
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- Cited (2)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502059 | KEAP1 antibody |
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| KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) | |||||||
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Mouse | IgG1 | OTI1B4 | Purified | Hu, Ms | F, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Top Selling product! Published recently! Highly validated antibody!
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| TA590238 | KEAP1 antibody |
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| Rabbit Polyclonal KEAP1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
50 µg /
€409.00
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| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody! |
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| AP32137PU-N | KEAP1 (41-53) antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | P, WB |
0.1 mg /
€380.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA319790 | KEAP1 (C-term) antibody |
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| Rabbit Polyclonal KEAP1 Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321818 | KEAP1 antibody |
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| Anti-KEAP1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA327189 | KEAP1 antibody |
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| Rabbit anti-KEAP1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA347947 | KEAP1 antibody |
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| Rabbit Polyclonal Anti-Keap1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA350545 | KEAP1 antibody |
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| Rabbit Polyclonal Anti-KEAP1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA501989 | KEAP1 antibody |
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| KEAP1 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2) | |||||||
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Mouse | IgG1 | OTI1G2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA502056 | KEAP1 antibody |
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| KEAP1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1) | |||||||
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Mouse | IgG1 | OTI1A1 | Purified | Hu | F, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP52328PU-N | KEAP1 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN202189 | KEAP1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN202189BN | KEAP1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN202189LP | KEAP1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN202189RB | KEAP1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN308748 | Keap1 - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN308748BN | Keap1 - mouse gene knockout kit via CRISPR |
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Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN308748LP | Keap1 - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN308748RB | Keap1 - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302189 | KEAP1 (transcript variant 1) |
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| Recombinant protein of human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Published recently! |
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