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ACOX1 antibody

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Principal name

ACOX1 antibody

Alternative names for ACOX1 antibody

AOX, Palmitoyl-CoA oxidase, Straight-chain acyl-CoA oxidase, Peroxisomal acyl-coenzyme A oxidase 1

SwissProt ID

P07872 (Rat), Q15067 (Human), Q3SZP5 (Bovin), Q9R0H0 (Mouse), Q9Z1N0 (Cavpo)

Gene ID

51 (ACOX1), 11430 (Acox1), 50681

Ncbi ID

9606, NP_001171968.1, NM_001185039, 10090, NM_015729, NM_017340, NM_004035, NM_007292

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ACOX1 antibody

ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. 
Function: Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA.
Cellular Location: Peroxisome. 
Tissue Location: Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.

PAB4367 - ACOX1
Western blot analysis of ACOX1 polyclonal antibody ( Cat # PAB4367 ) in mouse liver lysate . Data is kindly provided by Hye Won Kang and Dr . McGrane at the Univ . of Connecticut ( Storrs, CT ) .
PAB4368 - ACOX1
Western blot analysis of ACOX1 ( arrow ) using rabbit ACOX1 polyclonal antibody ( Cat # PAB4368 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the ACOX1 gene ( Lane 2 ) ( Origene Technologies ) .
H00000051-B01P - ACOX1
Immunofluorescence of purified MaxPab antibody to ACOX1 on HeLa cell. [antibody concentration 10 ug/ml]
H00000051-B01P - ACOX1
Western Blot analysis of ACOX1 expression in transfected 293T cell line (H00000051-T01) by ACOX1 MaxPab polyclonal antibody.Lane 1: ACOX1 transfected lysate(72.6 KDa).Lane 2: Non-transfected lysate.
AP12247PU-N - ACOX1
Western blot analysis of anti-ACOX1 Pab (Cat.#AP12247PU-N) in mouse liver lysate. Data is kindly provided by Hye Won Kang and Dr. McGrane at the Univ. of Connecticut (Storrs, CT).
AP12248PU-N - ACOX1
(LEFT) Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (Cat#AP12248PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (Cat#AP12248PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies).
H00000051-D01P - ACOX1
Western Blot analysis of ACOX1 expression in transfected 293T cell line (H00000051-T01) by ACOX1 MaxPab polyclonal antibody.Lane 1: ACOX1 transfected lysate(74.70 KDa).Lane 2: Non-transfected lysate.
AM33416PU-N - ACOX1
Immunohistochemistry analysis in Formalin Fixed and Paraffin Embedded human colon carcinomastained with ACOX1 Monoclonal Antibody Cat.-No AM33416PU-N (Clone 153CT43.1.1) followed by peroxidase conjugation of the secondary antibody and DAB staining.
AM33416PU-N - ACOX1
Confocal immunofluorescent analysis of ACOX1 Monoclonal Antibody Cat.-No AM33416PU-N (Clone 153CT43.1.1) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
AM33416PU-N - ACOX1
Western blot analysis in K562 cell line lysates using ACOX1 Monoclonal Antibody Cat.-No AM33416PU-N (Clone 153CT43.1.1) (15ug/lane). This demonstrates the ACOX1 antibody detected the ACOX1 protein (arrow). (1 µg/ml)1:390
TA324047 - ACOX1
Predicted band size: 74 kDa . Positive control: Mouse stomach, liver and kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: Mouse stomach tissue lysate Lane2: Mouse liver tissue lysate Lane3: Mouse kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA324047 - ACOX1
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ACOX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x 200)
TA324048 - ACOX1
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOX1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200)
TA324048 - ACOX1
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ACOX1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)
TA335908 - ACOX1
Host: Rabbit; Target Name: Paox; Sample Tissue: Rat Pancreas lysates; Antibody Dilution: 1.0 ug/ml

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACOX1 antibody
  Anti-ACOX1 rabbit polyclonal antibody  
Predicted band size: 74 kDa . Positive control: Mouse stomach, liver and kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: Mouse stomach tissue lysate Lane2: Mouse liver tissue lysate Lane3: Mouse kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ACOX1 antibody
  Anti-ACOX1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ACOX1 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ACOX1 antibody
Immunohistochemistry analysis in Formalin Fixed and Paraffin Embedded human colon carcinomastained with ACOX1 Monoclonal Antibody Cat.-No AM33416PU-N (Clone 153CT43.1.1) followed by peroxidase conjugation of the secondary antibody and DAB staining. Mouse IgG1 153CT43.1.1 Purified Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

ACOX1 (N-term) antibody
Western blot analysis of anti-ACOX1 Pab (Cat.#AP12247PU-N) in mouse liver lysate. Data is kindly provided by Hye Won Kang and Dr. McGrane at the Univ. of Connecticut (Storrs, CT). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

ACOX1 (C-term) antibody
(LEFT) Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (Cat#AP12248PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of ACOX1 (arrow) using rabbit polyclonal ACOX1 Antibody (C-term) (Cat#AP12248PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACOX1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

ACOX1 overexpression lysate
ACOX1 overexpression lysate  
0.1 mg / €315.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Acox1 (GFP-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Acox1 (GFP-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL  
200 µl / €1,410.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Acox1 (Myc-DDK-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Acox1 (Myc-DDK-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL  
200 µl / €1,260.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Acox1 (GFP-tagged ORF) - Rat acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Acox1 (GFP-tagged ORF) - Rat acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL  
200 µl / €1,410.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Acox1 (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Acox1 (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), 200 uL, >10^7 TU/mL  
200 µl / €1,270.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (5)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
  2. Monoclonal (1)
Target
  1. ACOX1 (5)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (3)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (5)
Host
  1. Rabbit (4)
  2. Mouse (1)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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