ALDH2 antibody
Principal name
ALDH2 antibody
Alternative names for ALDH2 antibody
Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDHI, ALDH-E2, ALDM
SwissProt ID
P05091 (Human), P11884 (Rat), P12762 (Horse), P20000 (Bovin), P47738 (Mouse), Q2XQV4 (Pig)
Ncbi ID
9606, NP_000681, NP_000681.2, NP_033786, NM_000690, NP_033786.1, NM_009656, NM_032416
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ALDH2 antibody
This protein belongs to the aldehyde dehydrogese family of proteins.Aldehyde dehydrogese is the second enzyme of the major oxidative pathway of alcohol metabolism.Two major liver isoforms of aldehyde dehydrogese,cytosolic and mitochondrial,can be distinguished by their electrophoretic mobilities,kinetic properties,and subcellular localizations.Most Caucasians have two major isozymes,while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme.A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme.The increased exposure to acetaldehyde in individuals with the catalytically ictive form may also confer greater susceptibility to many types of cancer.This gene encodes a mitochondrial isoform,which has a low Km for acetaldehydes,and is localized in mitochondrial matrix.Altertive splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq,Mar 2011]
General readings
1. Kimura M, Sawayama T, Matsushita S, Higuchi S, Kashima H, Association between personality traits and ALDH2 polymorphism in Japanese male alcoholics. Alcoholism, clinical and experimental research 2009 May 33 (5): 799-803.
PMID: 19298328
ALDH2 monoclonal antibody (M01), clone 1E5 Western Blot analysis of ALDH2 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged ALDH2 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
ALDH2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH2 expression in human liver.
Western Blot analysis of ALDH2 expression in transfected 293T cell line (H00000217-T01) by ALDH2 MaxPab polyclonal antibody.Lane 1: ALDH2 transfected lysate(56.40 KDa).Lane 2: Non-transfected lysate.
Recombinant human ALDH2, 18-517 aa: 15% SDS-PAGE (3 µg)
Human Liver (formalin-fixed, paraffin-embedded) stained with ALDH2 antibody AP22751PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of ALDH2 (arrow) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH2 gene (Lane 2)
Western blot analysis of ALDH2 expression in HegG2 (A); A549 (B) whole cell lysates.
Anti-ALDH2 mouse monoclonal antibody (TA500615) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2(RC200505).
Immunofluorescent staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody (TA500615).
HEK293T cells transfected with either RC200505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALDH2 antibody(TA500615), and then analyzed by flow cytometry.
ALDH2 Antibody (Center) (Cat.# TA302016) western blot analysis in A549 cell line,mouse liver and lung,rat liver lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH2 antibody (Center) (Cat.#TA302016), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ALDH2 Antibody (N-term) (Cat. #TA302017) western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).
IF image of A549 cell stained with ALDH2 Antibody (N-term)(Cat#TA302017).A549 cells were incubated with ALDH2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .ALDH2 immunoreactivity is localized to Mitochondrion significantly.
(0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1.5ug/ml) as the reporter with the capture rabbit antibody at 2.5ug/ml.
Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody(Cat. #TA324332). TA324332 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
ALDH2 Monoclonal Antibody (Cat. #TA324332) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#TA324332) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
ALDH2 Monoclonal Antibody (Cat. #TA324332) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Western blot analysis of extracts of Rat liver cell and Mouse lung cell using ALDH2 antibody.
Immunohistochemistry of paraffin-embedded mouse esophagus using ALDH2 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ALDH2 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using ALDH2 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of A549 cell using ALDH2 antibody.
Host: Rabbit; Target Name: Aldh2; Sample Tissue: Mouse Small Intestine lysates; Antibody Dilution: 1.0 ug/ml
Western blot detection of ALDH2 in Hela,3T3,C6,COS7,CHO-K1 and A549 cell lysates using ALDH2 mouse mAb (1:1000 diluted).Predicted band size:56KDa.Observed band size:56KDa.
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- Cited (1)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500615 | ALDH2 antibody |
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| ALDH2 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2) | |||||||
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Mouse | IgG3 | OTI4H2 | Purified | Hu | F, ICC/IF |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Published recently! Highly validated antibody!
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| TA327010 | ALDH2 antibody |
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| Rabbit anti-ALDH2 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA302016 | ALDH2 antibody |
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| Rabbit Polyclonal Antibody against ALDH2 (Center) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA302017 | ALDH2 (N-term) antibody |
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| Rabbit Polyclonal Antibody against ALDH2 (N-term) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA311134 | ALDH2 antibody |
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| Goat Anti-ALDH2 Antibody | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324332 | ALDH2 antibody |
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| Mouse monoclonal ALDH2 Antibody | |||||||
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Mouse | IgG1 | 138CT22.3.8 | Purified | Hu | F, ICC/IF |
0.4 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA346880 | ALDH2 antibody |
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| Mouse Monoclonal ALDH2 Antibody | |||||||
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Mouse | IgG1 | 8D4-D6-D11 | Purified | Hst | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AP22751PU-N | ALDH2 (N-term) antibody |
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Rabbit | Purified | Hu | F, ICC/IF, P, WB |
0.2 ml /
€425.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300505 | ALDH2 |
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| Recombinant protein of human aldehyde dehydrogenase 2 family (mitochondrial) (ALDH2), nuclear gene encoding mitochondrial protein | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400231 | ALDH2 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208315L2V | Lenti ORF particles, Aldh2 (GFP-tagged) - Mouse aldehyde dehydrogenase 2, mitochondrial (Aldh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,210.00
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| OriGene Technologies, Inc. | ||
| MR208315L1V | Lenti ORF particles, Aldh2 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 2, mitochondrial (Aldh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,080.00
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214002L2V | Lenti ORF particles, Aldh2 (GFP-tagged ORF) - Rat aldehyde dehydrogenase 2 family (mitochondrial) (Aldh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,210.00
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| OriGene Technologies, Inc. | ||
| RR214002L1V | Lenti ORF particles, Aldh2 (Myc-DDK-tagged ORF) - Rat aldehyde dehydrogenase 2 family (mitochondrial) (Aldh2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€1,090.00
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| OriGene Technologies, Inc. | ||
