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ALDH2 antibody

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Principal name

ALDH2 antibody

Alternative names for ALDH2 antibody

Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDHI, ALDH-E2, ALDM

SwissProt ID

P05091 (Human), P11884 (Rat), P12762 (Horse), P20000 (Bovin), P47738 (Mouse), Q2XQV4 (Pig)

Gene ID

217 (ALDH2), 11669 (Aldh2), 29539

Ncbi ID

9606, NP_000681, NP_000681.2, NP_033786, NM_000690, NP_033786.1, NM_009656, NM_032416

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Hst (Hamster)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALDH2 antibody

This protein belongs to the aldehyde dehydrogese family of proteins.Aldehyde dehydrogese is the second enzyme of the major oxidative pathway of alcohol metabolism.Two major liver isoforms of aldehyde dehydrogese,cytosolic and mitochondrial,can be distinguished by their electrophoretic mobilities,kinetic properties,and subcellular localizations.Most Caucasians have two major isozymes,while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme.A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme.The increased exposure to acetaldehyde in individuals with the catalytically ictive form may also confer greater susceptibility to many types of cancer.This gene encodes a mitochondrial isoform,which has a low Km for acetaldehydes,and is localized in mitochondrial matrix.Altertive splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq,Mar 2011]

General readings

1. Kimura M, Sawayama T, Matsushita S, Higuchi S, Kashima H, Association between personality traits and ALDH2 polymorphism in Japanese male alcoholics. Alcoholism, clinical and experimental research 2009 May 33 (5): 799-803.
PMID: 19298328

H00000217-M01 - ALDH2
ALDH2 monoclonal antibody (M01), clone 1E5 Western Blot analysis of ALDH2 expression in MCF-7 ( Cat # L046V1 ).
H00000217-M01 - ALDH2
Detection limit for recombinant GST tagged ALDH2 is approximately 0.3ng/ml as a capture antibody.
H00000217-M09 - ALDH2
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
H00000217-M10 - ALDH2
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
H00000217-M17 - ALDH2
Detection limit for recombinant GST tagged ALDH2 is approximately 1ng/ml as a capture antibody.
H00000217-D01P - ALDH2
ALDH2 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALDH2 expression in human liver.
H00000217-D01P - ALDH2
Western Blot analysis of ALDH2 expression in transfected 293T cell line (H00000217-T01) by ALDH2 MaxPab polyclonal antibody.Lane 1: ALDH2 transfected lysate(56.40 KDa).Lane 2: Non-transfected lysate.
AR09142PU-L - ALDH2
Recombinant human ALDH2, 18-517 aa: 15% SDS-PAGE (3 µg)
AP22751PU-N - ALDH2
Human Liver (formalin-fixed, paraffin-embedded) stained with ALDH2 antibody AP22751PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22751PU-N - ALDH2
Western blot analysis of ALDH2 (arrow) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH2 gene (Lane 2)
AR51923PU-N - ALDH2
CPA1040-100ul - ALDH2
Western blot analysis of ALDH2 expression in HegG2 (A); A549 (B) whole cell lysates.
TA500615 - ALDH2
Anti-ALDH2 mouse monoclonal antibody (TA500615) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2(RC200505).
TA500615 - ALDH2
Immunofluorescent staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody (TA500615).
TA500615 - ALDH2
TA500615 - ALDH2
HEK293T cells transfected with either RC200505 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALDH2 antibody(TA500615), and then analyzed by flow cytometry.
TP300505 - ALDH2
TA302016 - ALDH2
ALDH2 Antibody (Center) (Cat.# TA302016) western blot analysis in A549 cell line,mouse liver and lung,rat liver lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).
TA302016 - ALDH2
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH2 antibody (Center) (Cat.#TA302016), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA302017 - ALDH2
ALDH2 Antibody (N-term) (Cat. #TA302017) western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).This demonstrates the ALDH2 antibody detected the ALDH2 protein (arrow).
TA302017 - ALDH2
IF image of A549 cell stained with ALDH2 Antibody (N-term)(Cat#TA302017).A549 cells were incubated with ALDH2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .ALDH2 immunoreactivity is localized to Mitochondrion significantly.
TA311134 - ALDH2
(0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311134 - ALDH2
(1.5ug/ml) as the reporter with the capture rabbit antibody at 2.5ug/ml.
TA324332 - ALDH2
Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody(Cat. #TA324332). TA324332 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
TA324332 - ALDH2
ALDH2 Monoclonal Antibody (Cat. #TA324332) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
TA324332 - ALDH2
Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#TA324332) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
TA324332 - ALDH2
ALDH2 Monoclonal Antibody (Cat. #TA324332) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
TA327010 - ALDH2
Western blot analysis of extracts of Rat liver cell and Mouse lung cell using ALDH2 antibody.
TA327010 - ALDH2
Immunohistochemistry of paraffin-embedded mouse esophagus using ALDH2 antibody at dilution of 1:200 (400x lens).
TA327010 - ALDH2
Immunohistochemistry of paraffin-embedded human lung cancer using ALDH2 antibody at dilution of 1:200 (400x lens).
TA327010 - ALDH2
Immunohistochemistry of paraffin-embedded human stomach cancer using ALDH2 antibody at dilution of 1:200 (400x lens).
TA327010 - ALDH2
Immunofluorescence analysis of A549 cell using ALDH2 antibody.
TA344349 - ALDH2
Host: Rabbit; Target Name: Aldh2; Sample Tissue: Mouse Small Intestine lysates; Antibody Dilution: 1.0 ug/ml
TA346880 - ALDH2
Western blot detection of ALDH2 in Hela,3T3,C6,COS7,CHO-K1 and A549 cell lysates using ALDH2 mouse mAb (1:1000 diluted).Predicted band size:56KDa.Observed band size:56KDa.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALDH2 antibody
  Goat Anti-ALDH2 Antibody  
(0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+1 additional image

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