ALG2 antibody
Principal name
ALG2 antibody
Alternative names for ALG2 antibody
Alpha-1,3-mannosyltransferase ALG2, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, Asparagine-linked glycosylation protein 2
Ncbi ID
9606, NP_149078, NP_149078.1, NP_064382.3, NM_019998, NM_033087
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of ALG2 antibody
ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
"A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis." Thiel C., Schwarz M., Peng J., Grzmil M., Hasilik M., Braulke T., Kohlschuetter A., von Figura K., Lehle L., Koerner C. J. Biol. Chem. 278:22498-22505(2003)
Western Blot analysis of ALG2 expression in transfected 293T cell line (H00085365-T01) by ALG2 MaxPab polyclonal antibody.Lane1:ALG2 transfected lysate(45.76 KDa).Lane2:Non-transfected lysate.
Human HepG2; WB Suggested Anti-ALG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ALG2 antibody - C-terminal region (ARP48974_P050) in Human HepG2 cells using Western Blot
Western Blot analysis of ALG2 expression in transfected 293T cell line (H00085365-T01) by ALG2 MaxPab polyclonal antibody.Lane1:ALG2 transfected lysate(45.76 KDa).Lane2:Non-transfected lysate.
Human HepG2; WB Suggested Anti-ALG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ALG2 antibody - C-terminal region (AP43589PU-N) in Human HepG2 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
Flow cytometric Analysis of Jurkat cells, using anti-ALG2 antibody(TA504215),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504217)
Anti-ALG2 mouse monoclonal antibody (TA504217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALG2(RC204766).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALG2 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504218)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-ALG2 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ALG2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ALG2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ALG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ALG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504217 | ALG2 antibody |
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| ALG2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) | |||||||
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Mouse | IgG1 | OTI2A3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA504242 | ALG2 antibody |
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| ALG2 mouse monoclonal antibody, clone OTI3C2 (formerly 3C2) | |||||||
 |
Mouse | IgG1 | OTI3c2 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA322120 | ALG2 antibody |
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| Anti-ALG2 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322121 | ALG2 antibody |
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| Anti-ALG2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA504215 | ALG2 antibody |
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| ALG2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) | |||||||
 |
Mouse | IgG1 | OTI1C5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504218 | ALG2 antibody |
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| ALG2 mouse monoclonal antibody, clone OTI1E7 (formerly 1E7) | |||||||
 |
Mouse | IgG1 | OTI1E7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304766 | ALG2 (transcript variant 1) |
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| Recombinant protein of human asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae) (ALG2), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206559L2V | Lenti ORF particles, Alg2 (GFP-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206559L1V | Lenti ORF particles, Alg2 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||