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ALG2 antibody

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Principal name

ALG2 antibody

Alternative names for ALG2 antibody

Alpha-1,3-mannosyltransferase ALG2, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, Asparagine-linked glycosylation protein 2

SwissProt ID

Q9DBE8 (Mouse), Q9H553 (Human)

Gene ID

85365 (ALG2), 56737 (Alg2)

Ncbi ID

9606, NP_149078, NP_149078.1, NP_064382.3, NM_019998, NM_033087

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of ALG2 antibody

ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

"A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis." Thiel C., Schwarz M., Peng J., Grzmil M., Hasilik M., Braulke T., Kohlschuetter A., von Figura K., Lehle L., Koerner C. J. Biol. Chem. 278:22498-22505(2003)

H00085365-B01 - ALG2
Western Blot analysis of ALG2 expression in transfected 293T cell line (H00085365-T01) by ALG2 MaxPab polyclonal antibody.Lane1:ALG2 transfected lysate(45.76 KDa).Lane2:Non-transfected lysate.
ARP48974_P050 - ALG2
Human HepG2; WB Suggested Anti-ALG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ALG2 antibody - C-terminal region (ARP48974_P050) in Human HepG2 cells using Western Blot
H00085365-B01P - ALG2
Western Blot analysis of ALG2 expression in transfected 293T cell line (H00085365-T01) by ALG2 MaxPab polyclonal antibody.Lane1:ALG2 transfected lysate(45.76 KDa).Lane2:Non-transfected lysate.
AP43589PU-N - ALG2
Human HepG2; WB Suggested Anti-ALG2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ALG2 antibody - C-terminal region (AP43589PU-N) in Human HepG2 cells using Western Blot
TA504215 - ALG2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
TA504215 - ALG2
Flow cytometric Analysis of Jurkat cells, using anti-ALG2 antibody(TA504215),(Red), compared to a nonspecific negative control antibody,(Blue).
TA504217 - ALG2
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504217)
TA504217 - ALG2
Anti-ALG2 mouse monoclonal antibody (TA504217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALG2(RC204766).
TA504217 - ALG2
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALG2 monoclonal antibody.(1:200)
TA504217 - ALG2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
TA504218 - ALG2
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504218)
TA504218 - ALG2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
TA504218 - ALG2
Western blot analysis of extracts (10ug) from 1 cell line by using anti-ALG2 monoclonal antibody.(1:200)
TA504242 - ALG2
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
TA504242 - ALG2
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
TA504242 - ALG2
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504242)
TA504242 - ALG2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.
TA504242 - ALG2
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.
TP304766 - ALG2
TA322120 - ALG2
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ALG2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
TA322120 - ALG2
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ALG2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
TA322121 - ALG2
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ALG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x 200)
TA322121 - ALG2
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ALG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x200)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALG2 antibody
  Anti-ALG2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ALG2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ALG2 antibody
  Anti-ALG2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ALG2 antibody at dilution 1/35, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

2 Item(s)

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Product Type
  1. Primary Antibody (2)
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  1. ALG2 (2)
Reactivity
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  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Enzyme Immunoassay (E) (2)
  2. Paraffin Sections (P) (2)
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