Bax antibody
Principal name
Bax antibody
Alternative names for Bax antibody
Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)
Background of Bax antibody
The human protein Bax sits at a critical regulatory junction of apoptosis, or programmed cell death. Activated Bax changes conformation, inserts into the MOM (Mitochondrial Outer Membrane), oligomerizes, and induces MOM permeabilization, causing the release of cytochrome c, which effectively commits the cell to die. (Ma J et al., 2012). Mechanisms of membrane perforation include formation of hetero-oligomeric complexes of Bax with other pro-apoptotic proteins such as Bak, or formation of lipidic pores physically aided by mitochondrial membrane-inserted proteins (Garg P et al., 2012). Connexins play important roles in many physiological and pathological processes. In the context of apoptosis, Cx43 translocated to the mitochondria, where it interacted with Bax to initiate the mitochondrial apoptotic pathway. The 241-382 aa region of Cx43 was required for interaction with Bax. Furthermore, this region was responsible for permeabilizing mitochondrial membrane potential. Recent studies elucidate a novel mechanism of the Cx43-mediated regulation of apoptosis in pancreatic cancer (Sun Y et al., 2012).
Bax acts as a biomarker that exhibited a difference in sub-cellular localization between normal OCSE (Oral Cavity Squamous Epithelium) and OSCC (Oral Cavity Squamous Cell Carcinoma) and was also the only apoptotic protein significantly associated with prognosis. The translocation of Bax from the nucleus to the cytoplasm in OSCC is consistent with increased Bax function at the mitochondria, leading to improved sensitivity to radiotherapy-induced apoptosis in tumours with elevated Bax expression. Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signaling (Bose P et al., 2012).
General readings
1. Dinh C et al. Short Interfering RNA against Bax Attenuates TNFa-Induced Ototoxicity in Rat Organ of Corti Explants. Otolaryngol Head Neck Surg 148:834-40 (2013). ICC/IF ; Rat .
2. Monsalve DM et al. Human VRK2 modulates apoptosis by interaction with Bcl-xL and regulation of BAX gene expression. Cell Death Dis 4:e513 (2013). Human .
Western blot analysis of Bax expression in rat kidney tissue lysate.
Immunoperoxidase of monoclonal antibody to BAX on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between BCL2L1 and BAX. HeLa cells were stained with anti-BCL2L1 rabbit purified polyclonal 1:1200 and anti-BAX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to BAX on HeLa cell . [antibody concentration 10 ug/ml]
Human kidney; BAX antibody - N-terminal region (AVARP02020_T100) in Human kidney cells using Immunohistochemistry
Human Heart; . Rabbit Anti-BAX Antibody. . Catalog Number:. AVARP02020 . Paraffin Embedded Tissue: Human cardiac cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human, Mouse, Rat; Immunohistochemistry with NT2, mouse brain; rat brain. tissue; BAX antibody - N-terminal region (AVARP02020_T100) in Human, Mouse, Rat cells using Immunohistochemistry
Human NT-2 and Mouse WT brain; . Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Gene Name: BAX. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science.; BAX antibody - N-terminal region (AVARP02020_T100) in Human NT-2 and Mouse WT brain cells using Western Blot
Human HepG2; WB Suggested Anti-BAX Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; BAX antibody - N-terminal region (AVARP02020_T100) in Human HepG2 cells using Western Blot
Immunohistochemical analysis in paraffin-embedded human brain tissue using Bax antibody (Cat.-No. AP06027PU-N).
Western blot (WB) analysis of Bax antibody (Cat.-No.: AP06027PU-N) at 1/500 dilution
Lane1:MCF-7 cell lysate
Lane2:Raw264.7 cell lysate
Lane3:Rat brain tissue lysate
Lane1:MCF-7 cell lysate
Lane2:Raw264.7 cell lysate
Lane3:Rat brain tissue lysate
Immunoprecipitation of in vitro translated Bax-alpha (1) and Bax-beta (2) using Bax antibody, cln 6A7, Bax-alpha native (3) and denatured protein (4) using Bax-beta antibody, and Bax-beta native (5) and denatured (6) protein using Bax-beta antibody.
Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot detection against Immunogen.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-Bax (BH3 Domain) Pab (Cat.#AP11294PU-N) in A375 cell lysate. Bax BH3 Domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Normal breast stained with Anti-Bax antibody
Paraffin Sections of human normal breast tissue stained with anti-Bax Antibody Cat.-No AM11090PU (Clone SP47).
Flow Cytometric analysis of rabbit anti-Bax Antibody Cat.-No AM11090PU (Clone SP47) in HeLa (green) compare to negative control of rabbit IgG (blue).
Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of BAX expression in HeLa cell lysate.
Immunocytochemistry staining of HL-60 cells using BAX polyclonal antibody ( Cat # PAB12927 ) at 2 µg/ml.
Western blot analysis of BAX in HL-60 cell lysates with BAX polyclonal antibody ( Cat # PAB12927 ) at ( A ) 1, ( B ) 2, and ( C ) 4 µg/ml.
Western blot analysis of HeLa cell lysate. Using BAX polyclonal antibody ( Cat # PAB7955 ) .
Western blot analysis of BAX expression in rat kidney tissue lysate.
Western blot analysis of Bax antibody (Cat.-No AP21052PU-N) in extracts from HepG2 cells.
Human HepG2; WB Suggested Anti-BAX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; BAX antibody - N-terminal region (ARP58870_P050) in Human HepG2 cells using Western Blot
Western blot analysis of Hela cell lysis using BAX antibody
Western blot analysis of Bax protein in Mouse brain extract (A), Rat brain extract (B), HEK 293 cells (C). 200 μg of total protein loaded per well. GST-fusion recombinant Human Bax protein was tested under the same conditions at the concentrations of 100ng (D), 200ng (E), and 500ng (F).
Western blot analysis of Bax protein in Mouse brain extract (A), Rat brain extract (B), HEK 293 cells (C). 200 μg of total protein loaded per well. GST-fusion recombinant Human Bax protein was tested under the same conditions at the concentrations of 100ng (D), 200ng (E), and 500ng (F).
Formalin-Fixed, Paraffin-Embedded Human Uterus, Endometrium stained with BAX antibody Cat.-No AP31829PU-N
Immunofluorescence analysis of A549 cells, using BAX antibody Cat.-No AP31829PU-N. The picture on the right is treated with the synthesized peptide.
Formalin-Fixed, Paraffin-Embedded Hodgkin's lymphoma stained with Bax Antibody Cat.-No AM32861PU (Clone 2D2).
Western blot analysis of extracts from NIH3T3 cells using anti-Bax, clone 5B7.
Immunoprecipitation/Western blot analysis of extracts from Daudi cells(lane 1 and lane 2) and THP-1 cells (lane 4 and lane5). Lane 4 contains THP-1 lysate input (5%). Lane 1 and lane 4 were immunoprecipitated with mouse IgG1 isotype control antibodies and lane 2 and lane 5 were immunoprecipitated with BAX antibody (6A7). Western blot analysis was performed using rabbit anti-BAX antibody (poly6251).
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bax polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Formalin-paraffin Hodgkin's lymphoma stained with Bax Antibody Cat.-No AM50160PU (Clone SPM336).
Immunofluorescence staining of methanol-fixed A549 cells using Bax (Phospho-Thr167) Antibody AP55712PU-N.
Immunohistochemical analysis of BAX staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of BAX expression in MCF7 (A); HeLa (B); A431 (C); HEK293T (D); Raw264.7 (E); rat brain (F) whole cell lysates.
Immunohistochemical analysis of BAX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of BAX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of BAX expression in HeLa (A); A549 (B); A375 (C); Jurkat (D); HepG2 (E); PC12 (F) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAX Rabbit Polyclonal antibody. (TA590174)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BAX Rabbit Polyclonal antibody. (TA590174)
Western Blot: BAX Antibody - Western Blot was performed using affinity purified BAX antibody. The lanes contain 5-30ug of a breats tummor tissue whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer BAX antibody, aa(1-100) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (RC219954, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAX.
ELISA: BAX Antibody
Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using Rabbit polyclonal anti-BAX antibody at (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (RC204369, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyclonal anti-BAX antibody at 1:2000 dilution
Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 ug/mL.
Immunocytochemistry staining of HL-60 cells using Bax at 2 ug/mL.
Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 ug/mL.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using BAX antibody.The picture on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using BAX antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using BAX antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, using BAX antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using BAX antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using BAX antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HepG2 cells, using Bax (Ab-167) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of LOVO cells, using Bax (Ab-167) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, using Bax antibody (#TA312600).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Bax antibody (#TA312600).The picture on the right is treated with the synthesized peptide.
Predicted band size: 21 kDa . Positive control: Human brain malignant glioma tissue, MCF7, Raji, Lovo and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8+12%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2: MCF7 cell lysate Lane3: Raji cell lysate Lane4: Lovo cell lysate Lane5: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/426 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 7 seconds)
Predicted band size: 21 kDa . Positive control: Human brain malignant glioma tissue, MCF7, Raji and Lovo cell lysate. Recommended dilution: 1/500-2000. (Gel: 8+12%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2: MCF7 cell lysate Lane3: Raji cell lysate Lane4: Lovo cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 7 seconds)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using BAX antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis on HepG2 cell lysate using Bax Antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using Bax Antibody.
Western blot analysis of extracts of mouse liver cell line, using BAX antibody.
Immunohistochemistry of paraffin-embedded rat lung using BAX antibody at dilution of 1:200 (400x lens).
Western blot analysis of extracts from Hela cells (lane 1) and human PBMCs (lane 2) using anti-Bax, clone 2D2.
Western blot detection of Bax in COS7,Raji,CEM and Hela cell lysates using Bax mouse mAb (1:1000 diluted). Predicted band size:20KDa. Observed band size:20KDa.
Western blot analysis on HepG2 cell lysate using Bax Antibody
Immunoprecipitation/Western blot analysis of extracts from Daudi cells(lane 1 and lane 2) and THP-1 cells (lane 4 and lane5). Lane 4 contains THP-1 lysate input (5%). Lane 1 and lane 4 were immunoprecipitated with mouse IgG1 isotype control antibodies and lane 2 and lane 5 were immunoprecipitated with BAX antibody (6A7). Western blot analysis was performed using rabbit anti-BAX antibody (poly6251).
Western blot analysis of extracts from Hela cells (lane 1) and human PBMCs (lane 2) using anti-Bax, clone 2D2.
Immunofluorescence analysis of A549 cells, using Bax (Phospho-Thr167) antibody.The picture on the right is treated with the synthesized peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM32861PU-S | Bax (3-16) antibody |
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Mouse | IgG1 | 2D2 | Purified | Hu, Mky | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
Published recently! OriGene recommends this product for Immunohistochemistry. |
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| AM32861PU-T | Bax (3-16) antibody |
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|
Mouse | IgG1 | 2D2 | Purified | Hu, Mky | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
Published recently! OriGene recommends this product for Immunohistochemistry. |
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| TA590174 | Bax antibody |
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| Rabbit Polyclonal Bax Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| AM50160PU-S | Bax antibody |
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 |
Mouse | IgG1 | SPM336 | Purified | Hu, Mky | F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50160PU-T | Bax antibody |
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|
Mouse | IgG1 | SPM336 | Purified | Hu, Mky | F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| TA306176 | Bax (N-term) antibody |
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| Rabbit Polyclonal Bax Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA312594 | Bax antibody |
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| Rabbit polyclonal anti-BAX antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312595 | Bax antibody |
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| Rabbit polyclonal anti-BAX antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312596 | Bax antibody |
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| Rabbit polyclonal anti-BAX antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA312598 | Bax antibody |
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| Rabbit polyclonal Bax (Ab-167) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312599 | Bax antibody |
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| Rabbit polyclonal Bax (Thr167) antibody(Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312600 | Bax antibody |
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| Rabbit polyclonal anti-Bax antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321641 | Bax antibody |
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| Anti-BAX Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321642 | Bax antibody |
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| Anti-BAX Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325255 | Bax antibody |
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| Rabbit Polyclonal Bax Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA326770 | Bax antibody |
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| Bax Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA328048 | Bax antibody |
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| Mouse Monoclonal anti-Bax Antibody | |||||||
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Mouse | IgG1 | 2D2 | Purified | Hu | IP, P, WB |
0.1 mg /
€225.00
|
| OriGene Technologies, Inc. | |||||||
| TA328050 | Bax (12-24) antibody |
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| Mouse Monoclonal anti-Bax Antibody | |||||||
 |
Mouse | IgG1 | 6A7 | Purified | Hu, Mky, Ms, Rt | IP |
0.1 mg /
€225.00
|
| OriGene Technologies, Inc. | |||||||
| TA346891 | Bax antibody |
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| Mouse Monoclonal Bax Antibody | |||||||
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Mouse | IgG2a | 2B12-G6-H6 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347321 | Bax antibody |
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| Rabbit Polyclonal Anti-Bax Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
