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Carabin antibody

Principal name

Carabin antibody

Alternative names for Carabin antibody

TBC1D10C, TBC1 domain family member 10C

SwissProt ID

Q8C9V1 (Mouse), Q8IV04 (Human)

Gene ID

374403 (TBC1D10C), 108995 (Tbc1d10c)

Ncbi ID

NP_940919.1, 9606, NP_940919, NM_198517, NP_848765.2, NM_178650

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Carabin antibody

Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

Formalin fixed, paraffin embedded human spleen with Rabbit anti Human Carabin (AP05728PU-N) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).

AP05728PU-N

Western blot analysis of whole cell lysate from Daudi, Burkitt's lymphoma derived cells probed with Rabbit anti Human Carabin (AP05728PU-N) at 1(A) and 2(B) µg/ml

AP05728PU-N

TBC1D10C polyclonal antibody ( Cat # PAB7529 ) ( 2 µg/ml ) staining of human spleen lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7529

Immunohistochemistry of TBC1D10C in human spleen tissue with TBC1D10C polyclonal antibody ( Cat # PAB13276 ) at 2.5 µg/ml.

PAB13276

Western blot analysis of TBC1D10C in Daudi cell lysate with TBC1D10C polyclonal antibody ( Cat # PAB13276 ) at ( A ) 1 and ( B ) 2 µg/ml.

PAB13276

Immunohistochemistry of TBC1D10C in human spleen tissue with TBC1D10C polyclonal antibody ( Cat # PAB13277 ) at 2.5 µg/ml.

PAB13277

Western blot analysis of TBC1D10C in human spleen tissue lysate with TBC1D10C polyclonal antibody ( Cat # PAB13277 ) at ( A ) 1 and ( B ) 2 µg/ml.

PAB13277

Human Jurkat; WB Suggested Anti-TBC1D10C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; TBC1D10C antibody - N-terminal region (ARP55893_P050) in Human Jurkat cells using Western Blot

ARP55893_P050

Human Thymus; WB Suggested Anti-TBC1D10C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; TBC1D10C antibody - N-terminal region (ARP55894_P050) in Human Thymus cells using Western Blot