Caspase-1 antibody
Principal name
Caspase-1 antibody
Alternative names for Caspase-1 antibody
CASP-1, CASP1, IL1BC, IL1BCE, ICE, p45, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme
Ncbi ID
NP_001214.1, 9606, 7108, NP_033937, NP_001214, NP_033937.2, NP_150635.1, NP_150634.1, NM_033292, NM_033293, NM_033294, NM_001223, NP_150636, NP_150635, NP_150634
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)
Background of Caspase-1 antibody
Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEa), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-? inducing factor (IL-18) into their mature forms . Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-termil pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis . Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1ß and are resistant to endotoxic shock . At least six caspase-1 isoforms have been identified, including caspase-1 a, ß, ?, d, e and ? . Most caspase-1 isoforms (a, ß, ? and d) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 e typically contains only the p10 subunit, does not induce apoptosis and may act as a domint negative. The widely expressed ? isoform of caspase-1 induces apoptosis and lacks 39 amino-termil residues found in the a isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform desigted the "inflammasome" that includes caspase-5, Pycard/Asc, and LP1 .
General readings
Enari et al. Nature 375: 78, 1995.
Walker et al. Cell 78: 343, 1994.
Western blot analysis of Caspase-1 expression in Rat liver tissue lysate using Caspase-1 Antibody Cat.-No AP00006PU-N
Immunoperoxidase of monoclonal antibody to CASP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3ug/ml]
Detection limit for recombinant GST tagged CASP1 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemistry (IHC) analyzes of Caspase 1 antibody (Cat.-No.: AP06610PU-N) in paraffin-embedded human brain tissue.
Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for CASP1 polyclonal antibody ( Cat # PAB0217 ) . Using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.
Western blot analysis of CASP1 in HeLa cell lysate. Using CASP1 polyclonal antibody ( Cat # PAB0217 ) at 0.5 µg/ml.
Human caspase-1 precursor ( also designated ICE ) , non cross-reactive with caspase-1 p10 or caspase-1 p20.
Human placenta stained with Anti-Caspase 1 antibody
Western Blot analysis of CASP1 expression in transfected 293T cell line (H00000834-T02) by CASP1 MaxPab polyclonal antibody.Lane 1: CASP1 transfected lysate(35.00 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of CASP1 on formalin fixed, paraffin embedded human placenta with CASP1 polyclonal antibody.
Immunohistochemical staining of human heart tissue using CASP1 polyclonal antibody ( Cat # PAB13134 ) at 2 µg/ml.
Western blot analysis of CASP1 in human heart tissue lysate with CASP1 polyclonal antibody ( Cat # PAB13134 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Immunohistochemistry of CASP1 in human spleen tissue with CASP1 polyclonal antibody ( Cat # PAB13135 ) at 10 µg/ml.
Western blot analysis of CASP1 in HeLa cell lysate with CASP1 polyclonal antibody ( Cat # PAB13135 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Western blot analysis of CASP1 expression in rat liver tissue lysate.
Casp1 monoclonal antibody, clone 1H11 ( Cat # MAB3427 ) detect overexpressed Casp1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse Casp1 as a band of ~45 kDa.
Casp1 monoclonal antibody, clone 1H11 ( Cat # MAB3427 ) detect endogenous pro-Casp1 in spleen from WT mice, but not from spleen of Casp1 deficient mice. HSP-70 : loading control.
Casp1 monoclonal antibody, clone 1H11 ( Cat # MAB3427 ) detect overexpressed Casp1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse Casp1 by flow cytometry.
Human 721_B; WB Suggested Anti-CASP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. CASP1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; CASP1 antibody - middle region (ARP58983_P050) in Human 721_B cells using Western Blot
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with CASP1 antibody AP22755PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Heart (formalin-fixed, paraffin-embedded) stained with CASP1 antibody AP22860PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Testis (formalin-fixed, paraffin-embedded) stained with CASP1 antibody AP22860PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of caspase-1 in human heart cell lysate with caspase-1 antibody (AP22860PU-N) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Human Heart (formalin-fixed, paraffin-embedded) stained with CASP1 antibody AP22861PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with CASP1 antibody AP22861PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of caspase-1 in HeLa cell lysate with caspase-1 antibody (AP22861PU-N) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Western Blot analysis: MCF7 (Lane 1) and Jurkat (Lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Capsase-1 Antibody Cat.-No AM33084PU (Clone 5B10). Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence system.
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Immunohistochemical analysis of Caspase 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Caspase 1 expression in HEK293T PMA-treated (A); THP1 (B); HL60 (C); mouse lung (D); rat lung (E) whole cell lysates.
Immunohistochemical analysis of Caspase 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Caspase 1 expression in THP1 (A); HL60 (B); BAEC (C) whole cell lysates.
Western blot analysis of Caspase 1 p20 expression in HEK293T UV-treated (A); Raw264.7 LPS-treated (B); rat heart (C) whole cell lysates.
Immunohistochemical analysis of Caspase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Caspase 1 expression in A549 (A); Caco2 (B); THP1 (C) whole cell lysates.
Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 ug/ml.
Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 ug/ml.
Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 ug/mL.
Western blot analysis of extracts from K562 cells and HepG2 cells, using Caspase 1 (Cleaved-Asp210) antibody (#TA312620).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 1 (Cleaved-Asp210) antibody (#TA312620).The picture on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 1 (Cleaved-Asp210) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from rat eye cells, using CASP1 (p20, Cleaved-Asn120) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells, using Caspase 1 (p10, Cleaved-Ala317) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of Hela cell using TA323203(CASP1 Antibody) at dilution 1/150
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA323203(CASP1 Antibody) at dilution 1/10, the image on the right is treated with the fusion protein.(Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human pancreatic cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human pancreatic cancer tissue using CASP1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of extracts of various cell lines, using CASP1 antibody.
Western Blot: Caspase 1 Antibody [TA336408] - analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: Caspase 1 Antibody [TA336408] - Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for Caspase-1 expression (TA336408) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnificat
Western Blot: Caspase 1 Antibody (14F468) [TA336409] - analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, respectively.
Immunohistochemistry-Paraffin: Caspase 1 Antibody (14F468) [TA336409] - Formalin-fixed, paraffin-embedded adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is
Immunohistochemistry-Paraffin: Caspase 1 Antibody (14F468) [TA336409] - IHC-P detection of Caspase 1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).
Immunohistochemistry-Paraffin: Caspase 1 Antibody (14F468) [TA336409] - IHC-P detection of Caspase 1 in a section of normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in
Immunohistochemistry-Paraffin: Caspase 1 Antibody (14F468) [TA336409] - IHC-P detection of Caspase 1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct cytoplasmic staining along with som
Immunohistochemistry-Paraffin: Caspase 1 Antibody (14F468) [TA336409] - IHC-P detection of Caspase 1 protein in a section of normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, dif
Western blot analysis of Caspase 1 expression in 293 cells
Human Placenta stained with anti-Caspase-1 antibody.
Western blot analysis of Caspase 1 (Phospho-Ser376) expression in 293 cells
Western blot analysis of extracts from 293 cells, using Caspase 1 (Phospho-Ser376) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Caspase 1 (Phospho-Ser376) antibody.The picture on the right is treated with the synthesized peptide.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318982 | Caspase-1 (transcript variant delta) |
||||
| Recombinant protein of human caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) (CASP1), transcript variant delta | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760190 | Caspase-1 (full length, N-term HIS tag) |
||||
| Recombinant protein of human caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) (CASP1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||