Caspase-9 antibody
Principal name
Caspase-9 antibody
Alternative names for Caspase-9 antibody
CASP-9, CASP9, MCH6, ICE-LAP6, APAF-3, APAF3, ICE-like apoptotic protease 6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3
Ncbi ID
NP_001220.2, NP_001220, 9606, NP_056548, ABM82619.1, NM_001229, NM_032996, NP_127463, P55211, NP_056548.2, NM_015733
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E)
Background of Caspase-9 antibody
Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin.
Structure: Peptidase family C14, CARD domain. Heterotetramer, two isoforms, pro-Casp9 47 kD, active cleaved from Casp9 37 kD.
Distribution: Cytoplasm, when activated mitochondrial.
Function: Involved in caspase activation cascade responsible for apoptosis execution; cleaves/activates Casp3, Casp6.
Regulation: Inhibited by dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, Livin. Activated when recruited to Apaf-1/cytochrome c complex, cleaved by Apaf-1, granzyme B, Casp3, possibly Casp8 and -10 into large p37 and small p10 subunits.
Interaction: BIRC7; cleaves PARP, vimentin.
Western blot analysis of Caspase 9 with Rat kidney tissue lysate.
Western blot analysis of cleaved Caspase-9 in Jurkat cell lysate. Lane 1: Untreated; Lane 2: Etoposide treated.
Western blot analysis of Caspase-9 expression with mouse small intestine tissue lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between XIAP and CASP9 HeLa cells were stained with anti-XIAP rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to CASP9 on HeLa cell. [antibody concentration 10 ug/ml]
CASP9 monoclonal antibody (M01), clone 3B8-4G2 Western Blot analysis of CASP9 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged CASP9 is approximately 3ng/ml as a capture antibody.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and CASP9. Huh7 cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between BCL2L1 and CASP9 HeLa cells were stained with anti-BCL2L1 rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunohistochemistry (IHC) analyzes of Caspase 9 antibody (Cat.-No.: AP06377PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of Caspase 9 antibody (Cat.-No.: AP06377PU-N) in extracts from hela cells.
Western blot analysis using Caspase-9 Antibody Cat.-No AM05287PU-N (Clone LAP6 96-2-22) on MCF-7 cells negative (-) and positive (+) for caspase-3 and showing the proenzyme form of caspase-9 and one of the cleavage products after treatment with thapsigargin for 48 hours.
Immunocytochemical staining for H562 cells with Rabbit anti Human caspase-9 (AP05814PU-N)
Western blot analysis of whole cell lysates from HeLa cells probed with Rabbit anti Human caspase-9 (AP05814PU-N)
Western blot analysis of whole cell lysate from HeLa cells probed with Rabbit anti Human caspase-9 (AP05813PU-N)
Immunohistochemical analysis of CASP9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections. Using CASP9 polyclonal antibody ( Cat # PAB0248 ) at 1 : 2000. Hematoxylin-eosin counterstain. A : Brain striatum. B : Brain stem motor neuron.
Western blot analysis of CASP9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-CASP9 protein. Cleaved CASP9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9 : recombinant human pro-CASP9 ( full-length ) protein. PRO : pro-CASP9. L : large subunit of cleaved CASP9. S : small subunit of cleaved CASP9.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CASP9 (phospho S196) polyclonal antibody ( Cat # PAB0424 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The CASP9 (phospho S196) polyclonal antibody ( Cat # PAB0424 ) is used in Western blot to detect Phospho-CASP9-S196 in 293 ( left ) and Y-79 ( right ) cell lysates.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The anti-Phospho-Caspase 9-S196 Pab (Cat.#AP12575PU-N) is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.
Heart: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of CASP9 in HeLa whole cell lysate with CASP9 polyclonal antibody ( Cat # PAB13077 ) at 1 : 1000 dilution.
Western blot analysis of Casp9 expression in mouse intestine tissue lysate.
Western blot analysis of CASP9 polyclonal antibody in Jurkat cell lysate.Lane 1 : Untreated.Lane 2 : Etoposide treated.
Western blot analysis of CASP9 polyclonal antibody in rat kidney tissue lysate.
Western blot analysis of Casp9 polyclonal antibody in mouse small intestine tissue lysate.
Western blot analysis using CASP9 polyclonal antibody ( Cat # PAB9830 ) 0.5 µg/ml on K-562 cell lysate.
Western blot analysis using CASP9 polyclonal antibody ( Cat # PAB9831 ) at 0.5 mg/ml on HeLa whole cell lysate.
Western blot (WB) analysis of Caspase 9 (pGly140) antibody (Cat.-No.: AP20208PU-N) in extracts from hela cells.
Immunohistochemistry analyzes of Caspase 9 antibody (AP20294PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of Caspase 9 antibody (Cat.-No.: AP20294PU-N) in extracts from hela cells.
Immunohistochemistry analyzes of Caspase 9 antibody (Cat.-No.: AP20355PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of Caspase 9 antibody (Cat.-No.: AP20355PU-N) in extracts from HeLa cells.
AM20381PU-N Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
AM20381PU-N Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
AM20381PU-N Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunocytochemical staining of K562 cells using Caspase-9 antibody (AP22914PU-N) at 2 µg/ml.
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody (AP22914PU-N) at 1/1000 dilution.
Western blot analysis of extracts from 293 and Hela cells using Caspase 9 Antibody AP26062PU-N.
Western blot analysis of Caspase-9 fragments expression in apoptosis induced Jurkat cells by anti-Fas monoclonal antibody using AM26603AF-N. AM26603AF-N react with pro-caspase-9, Intermediate form and active form.
A431 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal Caspase-9 Antibody Cat.-No AM05287PU-S (Clone 96-2-22). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Western blot analysis of extracts from Hela cells, using anti-Caspase-9 Antibody Cat-No AP33260PU-N
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody AP55692PU-N (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody AP55692PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 9 expression in HeLa (A); NIH3T3 (B) whole cell lysates.
Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 9 expression in HeLa (A); HEK293T (B); SP2/0 (C); mouse kidney (D); rat lung (E) whole cell lysates.
Immunohistochemical analysis of Caspase 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 9 expression in HeLa (A); Jurkat (B) whole cell lysates.
ELISA: Caspase 9 Antibody
Immunohistochemical analysis of Caspase 9 p35 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Caspase 9 p35 expression in HepG2 Etoposide-treated (A); NIH3T3 Etoposide-treated (B) whole cell lysates.
Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution.
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution
Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 ug/mL.
Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.
Sample (30 ug of whole cell lysate). A: 293T. 10% SDS PAGE. TA308861 diluted at 1:1000.
The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.
Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 9 (Cleaved-Asp315) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Cleaved-Asp315) antibody.The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human cervical and stomach cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CASP9 antibody at dilution 1/50, on the right is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using CASP9 antibody at dilution 1/50. (Original magnification: x100)
Western blot analysis of Hela cell and mouse liver tissue using TA321308(CASP9 Antibody) at dilution 1/750
Immunohistochemistry of paraffin-embedded Human gastic cancer tissue using TA321308(CASP9 Antibody) at dilution 1/70. (Original magnification: ×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA321308(CASP9 Antibody) at dilution 1/70. (Original magnification: ×200)
Predicted cell location: Nucleus,Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using CASP9 (active) antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Nucleus,Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using CASP9 (active) antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x200)
CASP9 Antibody (Center) (Cat. #TA325012) western blot analysis in Hela cell line and mouse L929 tissue lysates (35ug/lane).This demonstrates the CASP9 antibody detected the CASP9 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center) (Cat. #TA325012), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of CASP9 Antibody (Center)(Cat#TA325012) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Caspase 9 expression in TNF-a treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using CASP9 antibody.
Immunohistochemistry-Paraffin: Caspase 9 Antibody [TA336326] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A. Section from a patient 24 hr after head trauma. B. Control: section from a patient with
Immunohistochemistry-Paraffin: Caspase 9 Antibody [TA336326] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A. Section from a dog brain 2 hr after reperfusion injury. B. ection from a dog brain sh
Immunohistochemistry-Paraffin: Caspase 9 Antibody [TA336326] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eo
Simple Western: Caspase 9 Antibody [TA336327] - Simple Western lane view shows a specific band detected for Caspase 9 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-44
Western Blot: Caspase 9 Antibody [TA336327] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identi
Immunohistochemistry: Caspase 9 Antibody [TA336327] - IHC analysis of AB expression in formalin-fixed, paraffin-embedded mouse brain tissue sections at 1:2000. Hematoxylin-eosin counterstain. A:Brain striatum, B: Brain stem motor neuron.
Western blot detection of Caspase-9 in A549,MCF7,Jurkat,K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.
Western blot detection of Caspase-9 in CHO-K1 cell lysate(B) and CHO-K1 transfected by Caspase-9(A) cell lysate using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49/37KDa.
Western blot analysis of Caspase 9 (Cleaved-Asp353) expression in NIH/3T3 cells treated with etoposide
Western blot analysis of Caspase 9 expression in Hela (A); Jurkat (B); HepG2 (C); mouse heart (D); rat heart (E) whole cell lysates.
Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation of Caspase 9 from 0.5mg Hela whole cell extract lysate; using Anti-Caspase 9 Antibody.
A431 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-caspase 9 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Western blot analysis of Caspase 9 phosphorylation expression in TNF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
The anti-Phospho-Caspase 9-S196 Pab (Cat. #TA324765) is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of extracts from COLO205 cells, using Caspase 9 (Phospho-Tyr153) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Caspase 9 (Phospho-Tyr153) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 5mins) and calyculinA (50ng/ml, 15mins), using Caspase 9 (Phospho-Thr125) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody.The picture on the right is treated with the synthesized peptide.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP313375 | Caspase-9 (transcript variant beta) |
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| Recombinant protein of human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant beta | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||