CD3 delta chain antibody
Principal name
CD3 delta chain antibody
Alternative names for CD3 delta chain antibody
CD3D, T3D, T-cell surface glycoprotein CD3 delta chain
SwissProt ID
P04234 (Human), P04235 (Mouse), P18438 (Sheep), P19377 (Rat), Q28072 (Bovin), Q764N2 (Pig), Q95LI8 (Macfa)
Ncbi ID
NP_000723, 9606, NP_000723.1, NP_037301.1, NP_038515.3, NM_000732, NM_001040651, NM_013169
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of CD3 delta chain antibody
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Western blot analysis of CD3-δ antibody (Cat.-No. AP20661PU-N) at 1/500 dilution.Lane 1: HEK293T whole cell lysate. Lane 2: NIH-3T3 whole cell lysate. Lane 3: PC12 whole cell lysate.
Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T01) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.81 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T02) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.81 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CD3D and CANX. HeLa cells were stained with anti-CD3D rabbit purified polyclonal 1:1200 and anti-CANX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
CD3D MaxPab rabbit polyclonal antibody. Western Blot analysis of CD3D expression in mouse kidney.
Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T01) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.90 KDa).Lane 2: Non-transfected lysate.
SM253P CD3 Antibody Staining of Paraffin Embedded Rat Spleen.
Immunofluorescent analysis of CD3d staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of CD3d expression in CCRFCEM (A); Jurkat (B); HuT78 (C) whole cell lysates.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-Biotin labled; followed by Streptavidin PE.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-APC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-PE-Cy5 labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody; followed by anti-mouse IgG PE.
Surface staining of normal human peripheral blood cells with Anti-Human gamma delta TCR FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte gate were used for analysis.
Staining of C57BL/6 lymph node cells with Anti-Mouse CD3 APC-Cy7 and 0.25 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.25 ug of Anti-Mouse gamma delta TCR Purified (right) followed by Anti-Armenian Hamster IgG FITC . Cells in the lymphocyte gate were used for analysis.
Predicted band size: 19 kDa . Positive control: Jurkat and HuT-78 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HuT-78 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Immunohistochemistry staining of Paraffin Tonsil tissue by CD3 antibody (dilution: 1:100 - 1:1000; visualization of staining: Membranous)
Immunohistochemistry staining of Paraffin Tonsil tissue by CD3 antibody (dilution: 1:100 - 1:500; visualization of staining: Membranous)
TA341375 (1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA320268 | CD3 delta chain antibody |
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| Mouse Anti-Human gamma delta TCR Purified (100 ug) | |||||||
 |
Mouse | B1.1 | Purified | Hu | F |
0.1 mg /
€185.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA320269 | CD3 delta chain antibody |
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| Hamster Anti-Mouse gamma delta TCR Purified (100 ug) | |||||||
 |
Hamster | eBioGL3 (GL-3, GL3) | Purified | Ms | F, IP |
0.1 mg /
€165.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321190 | CD3 delta chain antibody |
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| Anti-CD3D Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327632 | CD3 delta chain antibody |
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| Rabbit Polyclonal CD3 Antibody | |||||||
 |
Rabbit | Purified | Hu | P |
1 ml /
€585.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA327633 | CD3 delta chain antibody |
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| Rabbit Monoclonal CD3 Antibody | |||||||
 |
Rabbit | IgG1 | MRQ-39 | Purified | Hu | P |
1 ml /
€600.00
|
| OriGene Technologies, Inc. | |||||||
| TA341375 | CD3 delta chain antibody |
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| Goat Anti-CD3D (isoform A) Polyclonal Antibody | |||||||
 |
Goat | Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP20661PU-N | CD3 delta chain antibody |
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 |
Rabbit | Aff - Purified | Hu | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| SM253B | CD3 delta chain antibody |
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 |
Mouse | IgM | 1F4 | Biotin | Rt | F |
0.1 mg /
€400.00
|
| OriGene Technologies GmbH | |||||||
| SM253F | CD3 delta chain antibody |
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|
Mouse | IgM | 1F4 | FITC | Rt | F |
0.1 mg /
€400.00
|
| OriGene Technologies GmbH | |||||||
| SM253FS | CD3 delta chain antibody |
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|
Mouse | IgM | 1F4 | FITC | Rt | F |
50 µg /
€250.00
|
| OriGene Technologies GmbH | |||||||
| SM253P | CD3 delta chain antibody |
||||||
 |
Mouse | IgM | 1F4 | Purified | Rt | C, F, FN, IP, P |
0.2 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| SM253PS | CD3 delta chain antibody |
||||||
|
Mouse | IgM | 1F4 | Purified | Rt | C, F, FN, IP, P |
0.1 mg /
€330.00
|
| OriGene Technologies GmbH | |||||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212312L2V | Lenti ORF particles, Cd3d (GFP-tagged ORF) - Rat CD3 molecule delta polypeptide (Cd3d), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212312L1V | Lenti ORF particles, Cd3d (Myc-DDK-tagged ORF) - Rat CD3 molecule delta polypeptide (Cd3d), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||