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CD3 delta chain antibody

Principal name

CD3 delta chain antibody

Alternative names for CD3 delta chain antibody

CD3D, T3D, T-cell surface glycoprotein CD3 delta chain

SwissProt ID

P04234 (Human), P04235 (Mouse), P18438 (Sheep), P19377 (Rat), Q28072 (Bovin), Q764N2 (Pig), Q95LI8 (Macfa)

Gene ID

915 (CD3D), 25710 (Cd3d), 12500

Ncbi ID

NP_000723, 9606, NP_000723.1, NP_037301.1, NP_038515.3, NM_000732, NM_001040651, NM_013169

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Hamster, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD3 delta chain antibody

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Western blot analysis of CD3-δ antibody (Cat.-No. AP20661PU-N) at 1/500 dilution.Lane 1: HEK293T whole cell lysate. Lane 2: NIH-3T3 whole cell lysate. Lane 3: PC12 whole cell lysate.

AP20661PU-N

Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T01) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.81 KDa).Lane 2: Non-transfected lysate.

H00000915-B01

Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T02) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.81 KDa).Lane 2: Non-transfected lysate.

H00000915-B01P

Proximity Ligation Analysis (PLA) of protein-protein interactions between CD3D and CANX. HeLa cells were stained with anti-CD3D rabbit purified polyclonal 1:1200 and anti-CANX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00000915-D01P

CD3D MaxPab rabbit polyclonal antibody. Western Blot analysis of CD3D expression in mouse kidney.

H00000915-D01P

Western Blot analysis of CD3D expression in transfected 293T cell line (H00000915-T01) by CD3D MaxPab polyclonal antibody.Lane 1: CD3D transfected lysate(18.90 KDa).Lane 2: Non-transfected lysate.

H00000915-D01P

SM253P CD3 Antibody Staining of Paraffin Embedded Rat Spleen.

SM253P

Immunofluorescent analysis of CD3d staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of CD3d expression in CCRFCEM (A); Jurkat (B); HuT78 (C) whole cell lysates.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-Biotin labled; followed by Streptavidin PE.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-APC labled.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-FITC labled.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-PE labled.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody-PE-Cy5 labled.

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody; followed by anti-mouse IgG PE.

Surface staining of normal human peripheral blood cells with Anti-Human gamma delta TCR FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte gate were used for analysis.

TA320268

Staining of C57BL/6 lymph node cells with Anti-Mouse CD3 APC-Cy7 and 0.25 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.25 ug of Anti-Mouse gamma delta TCR Purified (right) followed by Anti-Armenian Hamster IgG FITC . Cells in the lymphocyte gate were used for analysis.

TA320269

Predicted band size: 19 kDa . Positive control: Jurkat and HuT-78 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HuT-78 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321190

Immunohistochemistry staining of Paraffin Tonsil tissue by CD3 antibody (dilution: 1:100 - 1:1000; visualization of staining: Membranous)

TA327632

Immunohistochemistry staining of Paraffin Tonsil tissue by CD3 antibody (dilution: 1:100 - 1:500; visualization of staining: Membranous)

TA327633

TA341375 (1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA341375

Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC

TA352720

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA321190	CD3 delta chain antibody
	Anti-CD3D Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA341375	CD3 delta chain antibody
	Goat Anti-CD3D (isoform A) Polyclonal Antibody
	Goat		Purified	Hu	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
AP20661PU-N	CD3 delta chain antibody
	Rabbit		Aff - Purified	Hu	WB	0.1 mg / €370.00
						OriGene Technologies GmbH

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