CD70 antibody
Principal name
CD70 antibody
Alternative names for CD70 antibody
CD27 ligand, CD27L, CD27LG, TNFSF7, Tumor necrosis factor ligand superfamily member 7
Ncbi ID
9606, NP_001243, NP_035747.1, NM_001252, NP_001243.1, AAH00725.1, NM_011617
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of CD70 antibody
CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. The provided human sCD27L protein contains the extracellular domain plus an N-terminal His-Tag.
General readings
Welten SP., et al. (2013) J. Virol. 87:6851-6865.Polak ME., et al. (2012) J. Invest. Dermatol. 132:1636-1644.
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 (Cat.-No AM05527PU), red in A and Mouse anti Human CD21 antibody, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 (Cat.-No AM05527PU) followed by the Histar setection system. High power.
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 (Cat.-No AM05527PU) followed by the Histar setection system. Medium power.
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 (Cat.-No AM05527PU) followed by the Histar setection system. Low power
Western Blot analysis of CD70 expression in transfected 293T cell line (H00000970-T01) by CD70 MaxPab polyclonal antibody.Lane 1: TNFSF7 transfected lysate(21.23 KDa).Lane 2: Non-transfected lysate.
Human ACHN; WB Suggested Anti-CD70 Antibody. . Titration: 1.0 ug/ml. . Positive Control: ACHN Whole Cell. CD70 is supported by BioGPS gene expression data to be expressed in ACHN.; CD70 antibody - N-terminal region (ARP61309_P050) in Human ACHN cells using Western Blot
Western blot analysis of CD70 expression in Jurkat (A); NCIH460 (B) whole cell lysates.
Sample (30 ug of whole cell lysate). A: JurKat. 12% SDS PAGE. CD27L (CD70) antibody. TA308292 diluted at 1:1000.
Western blot analysis of extracts from HuvEC, K562, HepG2, Jurkat, HeLa and COLO205 cells, using CD70 antibody.The lane on the right is treated with the synthesized peptide.
Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD70 antibody IHC of human tonsil.. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Surface staining of LPS and Anti-Mouse/Rat CD40 Functional Grade Purified stimulated mouse splenocytes with Anti-Mouse CD70 PE . Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Predicted band size: 21 kDa . Positive control: NCI-H460 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using CD70 antibody.
Immunofluorescence analysis of U2OS cell using CD70 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of CD70 expression in COLO205 cells
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (CD70 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (CD70 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
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- Cited (1)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM05527PU-T | CD70 antibody |
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Mouse | IgG1 | Bu69 | Purified | Hu | C, F |
25 µg /
€180.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA308292 | CD70 antibody |
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| Rabbit polyclonal antibody to CD27L (CD27L (CD70)) (CD27L (CD70) molecule) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| TA311855 | CD70 antibody |
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| Rabbit polyclonal anti-CD70 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA318843 | CD70 antibody |
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| CD70 Rabbit Polyclonal (Internal) Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Mky |
50 µg /
€425.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA318844 | CD70 antibody |
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| CD70 Rabbit Polyclonal (Internal) Antibody | |||||||
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Rabbit | IgG | Purified | Gorilla, Hu, Mky |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA318845 | CD70 antibody |
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| CD70 Rabbit Polyclonal (N-Terminus) Antibody | |||||||
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Rabbit | IgG | Purified | Hu |
50 µg /
€425.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA320290 | CD70 antibody |
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| Rat Anti-Mouse CD70 Purified (100 ug) | |||||||
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Rat | FR70 | Purified | Ms | F |
0.1 mg /
€175.00
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| OriGene Technologies, Inc. | |||||||
| TA321314 | CD70 antibody |
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| Anti-CD70 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA327233 | CD70 antibody |
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| Rabbit anti-CD70 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347946 | CD70 antibody |
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| Rabbit Polyclonal Anti-CD70 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351933 | CD70 antibody |
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| Rabbit Polyclonal Anti-CD70 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM05527PU-N | CD70 antibody |
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Mouse | IgG1 | Bu69 | Purified | Hu | C, F |
0.2 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| AM26803PU-N | CD70 antibody |
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Mouse | IgG3 | Ki-24 | Purified | Hu | F |
0.1 mg /
€210.00
|
| OriGene Technologies GmbH | |||||||
| AM26803RP-N | CD70 antibody |
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Mouse | IgG3 | Ki-24 | PE | Hu | F |
100 Tests /
€290.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300410 | CD70 |
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| Recombinant protein of human CD70 molecule (CD70) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Published recently! |
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| TP723392 | CD70 |
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| Purified recombinant protein of Human CD70 molecule (CD70). | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
CHO cells |
10 µg /
€135.00
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| OriGene Technologies, Inc. | |||||
| AR52063PU-N | CD70 (39-193, His-tag) |
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Human | Purified | > 90 % by SDS - PAGE |
0.25 mg /
€1,400.00
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| OriGene Technologies GmbH | |||||
| AR52063PU-S | CD70 (39-193, His-tag) |
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Human | Purified | > 90 % by SDS - PAGE |
50 µg /
€400.00
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| OriGene Technologies GmbH | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100785 | Mouse CD70/TNFSF7/cd27L ELISA Kit |
|
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1 kit /
€500.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY400501 | CD70 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
