CDK5 antibody
Principal name
CDK5 antibody
Alternative names for CDK5 antibody
PSSALRE, TPKII catalytic subunit, Cell division protein kinase 5,Cyclin-dependent kinase 5, Tau protein kinase II catalytic subunit, Serine/threonine-protein kinase PSSALRE
Ncbi ID
NP_001157882.1, 9606, NP_004926, NM_004935, NP_004926.1 (Gene ID : 1020), NM_001164410, NP_543161, NP_031694.1, NM_080885
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)
Background of CDK5 antibody
Cdks (cyclin-dependent kises) are heteromeric serine/threonine kises that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, D polymerase and thymidine kise. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kises and phosphastases, including Wee1, CDK-activating kise and Cdc25 phosphatase. In addition, cyclin expression is induced by molecular sigls at specific points of the cell cycle, leading to activation of Cdks. Tight control of Cdks is essential as misregulation can induce unscheduled proliferation, and genomic and chromosomal instability. Cdk4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma. Cdks are currently under investigation as potential targets for antineoplastic therapy, but as Cdks are essential for driving each cell cycle phase, therapeutic strategies that block Cdk activity are unlikely to selectively target tumor cells.
General readings
Demetrick DJ. et al. (1994) Cytogenet Cell Genet. 66:72-74.Meyerson M. et al. (1992) EMBO J. 11:2909-2917.
Human Jurkat; WB Suggested Anti-CDK5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; CDK5 antibody - N-terminal region (ARP38525_T100) in Human Jurkat cells using Western Blot
Zebrafish Embryo; . Sample Type: Zebrafish Embryo. Dilution:. 1:100, 1:200; CDK5 antibody - C-terminal region (ARP38526_T100) in Zebrafish Embryo cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-CDK5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; CDK5 antibody - C-terminal region (ARP38526_T100) in Human HepG2 cells using Western Blot
Western Blot analysis of CDK5 expression in transfected 293T cell line (H00001020-T02) by CDK5 MaxPab polyclonal antibody.Lane 1: CDK5 transfected lysate(32.12 KDa).Lane 2: Non-transfected lysate.
Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunoblot analysis of cell and tissue lysates. Lane 1 : SH-SY5Y cell lysate and lane 2 : mouse testis. ( *N.S : Nonspecific band )
CDK5 monoclonal antibody (M01A), clone 1A2. Western Blot analysis of CDK5 expression in human lung cancer.
Immunoblot analysis of cell and tissue lysates. Lane 1 : SH-SY5Y cell lysate, lane 2 : SK-N-SH cell lysate and lane 3 : mouse brain tissue lysate.
Immunoblot analysis of cell and tissue lysates. Lane 1 : SH-SY5Y cell lysate, lane 2 : SK-N-SH cell lysate and lane 3 : mouse brain tissue lysate.
Immunofluorescence of purified MaxPab antibody to CDK5 on HeLa cell. [antibody concentration 10 ug/ml]
CDK5 MaxPab polyclonal antibody. Western Blot analysis of CDK5 expression in rat brain.
Western Blot analysis of CDK5 expression in transfected 293T cell line (H00001020-T01) by CDK5 MaxPab polyclonal antibody.Lane 1: CDK5 transfected lysate(32.12 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CDK5 expression in transfected 293T cell line (H00001020-T02) by CDK5 MaxPab polyclonal antibody.Lane 1: CDK5 transfected lysate(33.30 KDa).Lane 2: Non-transfected lysate.
CDK5 MaxPab rabbit polyclonal antibody. Western Blot analysis of CDK5 expression in HeLa.
Immunohistochemistry (IHC) analyzes of Cdk5 antibody (Cat.-No.: AP01458PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of Cdk5 antibody (Cat.-No.: AP01458PU-N) in extracts from HepG2 cells.
Immunohistochemical staining with CDK5 polyclonal antibody ( Cat # PAB9990 ) was diluted 1 : 500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Human 721_B; WB Suggested Anti-CDK5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. CDK5 is supported by BioGPS gene expression data to be expressed in 721_B.; CDK5 antibody - N-terminal region (AVARP03025_P050) in Human 721_B cells using Western Blot
CDK5 MaxPab rabbit polyclonal antibody. Western Blot analysis of CDK5 expression in HeLa.
Immunoprecipitation of CDK5 transfected lysate using anti-CDK5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CDK5 MaxPab mouse polyclonal antibody (B01) (H00001020-B01).
Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.
Immunofluorescence analysis of GC7901 cells using CDK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng)
Flow cytometric analysis of K562 cells using CDK5 mouse mAb (green) and negative control (purple).
Western blot analysis of extracts from COS7 cells treated with EGF using Phospho-CDK5 (Tyr15) antibody AP55874PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescent staining of HT29 cells using anti-CDK5 mouse monoclonal antibody (TA500397).
Anti-CDK5 mouse monoclonal antibody (TA500397) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK5(RC200342).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK5 monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CDK5 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (RC200342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5.
HEK293T cells transfected with either RC200342 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDK5 antibody(TA500397), and then analyzed by flow cytometry.
Western blot of rat caudate lysate showing specific immunolabeling of the ~ 28k Cdk5 protein.
Western blot analysis of extracts from Jurkat cells and HepG2 cells, using CDK5 (Ab-15) antibodyThe lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using CDK5 (Ab-15) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDK5 (Ab-15) antibody.The picture on the right is treated with the synthesized peptide.
anti-CDK5 antibody was diluted 1:500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot analysis of Jurkat and NIH/3T3 cell using TA323961(CDK5 Antibody) at dilution 1/300
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323961(CDK5 Antibody)(left image) at dilution 1/30, the image on the right is treated with the fusion protein.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human brain tissue using TA323961(CDK5 Antibody)(left image) at dilution 1/30, the image on the right is treated with the fusion protein.(Original magnification: x200)
Western blot analysis of Human fetal brain and colon cancer tissue using TA323962(CDK5 Antibody) at dilution 1/150
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323962(CDK5 Antibody)(left image) at dilution 1/10, the image on the right is treated with the fusion protein.(Original magnification: x200)
Western blot analysis of CDK5 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using CDK5 antibody.
Western blot detection of CDK5(N-terminus) in 3T3,Hela,PC-12,COS7,Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.
Immunocytochemistry of HeLa cells using anti-CDK5(N-terminus) mouse mAb diluted 1:150
Western blot analysis of CDK5 phosphorylation expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500397 | CDK5 antibody |
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| CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) | |||||||
 |
Mouse | IgG1 | OTI2H6 | Purified | Can, Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| AM06622SU-N | CDK5 antibody |
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 |
Mouse | IgG1 | 4E4 | Ascites | Hu, Mky, Rt | E, ICC/IF, P, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA309090 | CDK5 antibody |
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| Mouse Anti-CDK5 Antibody | |||||||
 |
Mouse | IgG | 1H3 | Aff - Purified | Hu, Ms, Rt | C, WB |
0.1 ml /
€405.00
|
| OriGene Technologies, Inc. | |||||||
| TA312176 | CDK5 antibody |
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| Rabbit polyclonal CDK5 (Ab-15) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA319183 | CDK5 antibody |
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| Rabbit polyclonal anti-Cdk5 antibody | |||||||
 |
Rabbit | Serum | Hu, Ms, Rt | E, IP, WB |
0.1 ml /
€515.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA323961 | CDK5 antibody |
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| Anti-CDK5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA323962 | CDK5 antibody |
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| Anti-CDK5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA325346 | CDK5 antibody |
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| Rabbit Polyclonal CDK5 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325347 | CDK5 antibody |
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| Rabbit Polyclonal CDK5 (Tyr15) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332868 | CDK5 antibody |
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| Rabbit anti-CDK5 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA346910 | CDK5 antibody |
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| Mouse Monoclonal CDK5(N-terminus) Antibody | |||||||
 |
Mouse | IgG1 | 2E8-F9-B7-C11 | Purified | ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP01458PU-N | CDK5 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP07567SU-N | CDK5 (C-term) antibody |
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 |
Rabbit | Hu, Ms, Rt | E, IP, P, WB |
50 µl /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP55874PU-N | CDK5 pTyr15 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€300.00
|
||
| OriGene Technologies GmbH | |||||||
| AP55874PU-S | CDK5 pTyr15 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
50 µg /
€230.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200342 | CDK5 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200342BN | CDK5 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200342LP | CDK5 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200342RB | CDK5 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303042 | Cdk5 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
