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CKBB antibody

Principal name

CKBB antibody

Alternative names for CKBB antibody

Creatine kinase B-type, Creatine kinase B chain, B-CK, Creatine kinase BB, CKB

SwissProt ID

P00567 (Rabit), P05122 (Chick), P05124 (Canfa), P07335 (Rat), P12277 (Human), Q04447 (Mouse), Q29594 (Pig), Q5EA61 (Bovin)

Gene ID

1152 (CKB), 12709 (Ckb), 24264

Ncbi ID

NM_001823.3, 9606, NP_001814, NP_001814.2, NP_067248.1, NM_021273, NM_012529, NM_001823

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of CKBB antibody

Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes.
Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.

General readings

Chang EJ., et al. (2008) Nat Med. 14(9):966-72.
Debrincat MA., et al. (2007) J Biol Chem. 282(7):4728-37.

Immunofluorescence of monoclonal antibody to CKB on HeLa cell. [antibody concentration 30 ug/ml]

H00001152-M01

The CKB polyclonal antibody ( Cat # PAB4594 ) is used in Western blot to detect CKB in mouse colon tissue lysate .

PAB4594

The CKB polyclonal antibody ( Cat # PAB4594 ) is used in Western blot to detect CKB in Y-79 cell lysate .

PAB4594

The CKB polyclonal antibody ( Cat # PAB4595 ) is used in Western blot to detect CKB in mouse colon tissue lysate .

PAB4595

The CKB polyclonal antibody ( Cat # PAB4595 ) is used in Western blot to detect CKB in Y-79 cell lysate .

PAB4595

CKB polyclonal antibody ( Cat # PAB6995 ) ( 0.1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6995

Immunoblot analysis of human plasma protein. Lane 1 : Recombinant CK-MM(CKM) protein isolated from E. coli. Lane 2 : Recombinant CK-BB(CKB) protein isolated from E. coli. Lane 3 : Recombinant CK-MB(CKB, CKM) protein isolated from E. coli. Lane 4 : Human CK-MB(CKB, CKM) protein.

MAB0825

The anti-CKB Pab is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).

AP13617PU-N

The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).

AP13618PU-N

Recombinant human CKB, 1-381 aa, His-tagged

TP303669

TA302453 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA302453

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308722 diluted at 1:10000.

TA308722

Sample(30 ug of whole cell lysate). A:H1299. B:HeLa S3. 12% SDS PAGE. TA308722 diluted at 1:1000

TA308722

Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and blotted with Creatine kinase (brain) antibody (TA308722) diluted by 1:1000

TA308722

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain)(TA308722) antibody at 1:100 dilution.

TA308722

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB(TA308722) antibody at 1:100 dilution.

TA308722

Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA325138) western blot analysis in MDA-MB453,293,Y79 cell line ,mouse stomach and brain tissue lysates (35ug/lane).This demonstrates the CKB antibody detected the CKB protein (arrow).

TA325138

Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA328113) western blot analysis in MDA-MB-453,293 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the Creatine Kinase BB (CKB) antibody detected the Creatine Kinase BB (CKB) protein (arrow).

TA328113

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA308722	CKBB antibody
	Rabbit Polyclonal antibody to Creatine kinase (brain) (creatine kinase, brain)
	Rabbit	IgG		Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
TA302453	CKBB antibody
	Goat Polyclonal Antibody against CKB / Brain Creatine Kinase
	Goat			Aff - Purified	Can, Hu, Ms, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA325138	CKBB antibody
	Mouse monoclonal Creatine Kinase BB Antibody (Center)
	Mouse	IgG1	893CT29.1.1	Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
TA328113	CKBB antibody
	Mouse monoclonal anti-Creatine Kinase BB (CKB) antibody (Center)(Ascites)
	Mouse	IgG1	893CT29.1.1	Ascites	Hu, Ms	WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
AM26243BT-N	CKBB (N-term) antibody
	Mouse	IgG2b	CK-BYK/21E10	Biotin	Hu, Ms, Rb, Rt	C, ICC/IF, P, WB	50 µg / €290.00
							OriGene Technologies GmbH
AM26243PU-N	CKBB (N-term) antibody
	Mouse	IgG2b	CK-BYK/21E10	Purified	Hu, Ms, Rb, Rt	C, ICC/IF, P, WB	0.1 mg / €390.00
							OriGene Technologies GmbH
AP13617PU-N	CKBB (N-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
AP13618PU-N	CKBB antibody
	Rabbit	Ig		Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP303669	CKBB
	Recombinant protein of human creatine kinase, brain (CKB)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR205953L1V	Lenti ORF particles, Ckb (Myc-DDK-tagged) - Mouse creatine kinase, brain (Ckb), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR210613L2V	Lenti ORF particles, Ckb (GFP-tagged ORF) - Rat creatine kinase, brain (Ckb), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR210613L1V	Lenti ORF particles, Ckb (Myc-DDK-tagged ORF) - Rat creatine kinase, brain (Ckb), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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