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CKBB antibody

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Principal name

CKBB antibody

Alternative names for CKBB antibody

Creatine kinase B-type, Creatine kinase B chain, B-CK, Creatine kinase BB, CKB

SwissProt ID

P00567 (Rabit), P05122 (Chick), P05124 (Canfa), P07335 (Rat), P12277 (Human), Q04447 (Mouse), Q29594 (Pig), Q5EA61 (Bovin)

Gene ID

1152 (CKB), 12709 (Ckb), 24264

Ncbi ID

NM_001823.3, 9606, NP_001814, NP_001814.2, NP_067248.1, NM_021273, NM_012529, NM_001823

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of CKBB antibody

Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes.
Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.

General readings

Chang EJ., et al. (2008) Nat Med. 14(9):966-72.
Debrincat MA., et al. (2007) J Biol Chem. 282(7):4728-37.

H00001152-M01 - CKBB
Immunofluorescence of monoclonal antibody to CKB on HeLa cell. [antibody concentration 30 ug/ml]
PAB4594 - CKBB
The CKB polyclonal antibody ( Cat # PAB4594 ) is used in Western blot to detect CKB in mouse colon tissue lysate .
PAB4594 - CKBB
The CKB polyclonal antibody ( Cat # PAB4594 ) is used in Western blot to detect CKB in Y-79 cell lysate .
PAB4595 - CKBB
The CKB polyclonal antibody ( Cat # PAB4595 ) is used in Western blot to detect CKB in mouse colon tissue lysate .
PAB4595 - CKBB
The CKB polyclonal antibody ( Cat # PAB4595 ) is used in Western blot to detect CKB in Y-79 cell lysate .
PAB6995 - CKBB
CKB polyclonal antibody ( Cat # PAB6995 ) ( 0.1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
MAB0825 - CKBB
Immunoblot analysis of human plasma protein. Lane 1 : Recombinant CK-MM(CKM) protein isolated from E. coli. Lane 2 : Recombinant CK-BB(CKB) protein isolated from E. coli. Lane 3 : Recombinant CK-MB(CKB, CKM) protein isolated from E. coli. Lane 4 : Human CK-MB(CKB, CKM) protein.
AP13617PU-N - CKBB
The anti-CKB Pab is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).
AP13618PU-N - CKBB
The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).
AR09563PU-L - CKBB
AR09563PU-L - CKBB
Recombinant human CKB, 1-381 aa, His-tagged
TP303669 - CKBB
TA302453 - CKBB
TA302453 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA308722 - CKBB
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308722 diluted at 1:10000.
TA308722 - CKBB
Sample(30 ug of whole cell lysate). A:H1299. B:HeLa S3. 12% SDS PAGE. TA308722 diluted at 1:1000
TA308722 - CKBB
Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and blotted with Creatine kinase (brain) antibody (TA308722) diluted by 1:1000
TA308722 - CKBB
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain)(TA308722) antibody at 1:100 dilution.
TA308722 - CKBB
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB(TA308722) antibody at 1:100 dilution.
TA325138 - CKBB
Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA325138) western blot analysis in MDA-MB453,293,Y79 cell line ,mouse stomach and brain tissue lysates (35ug/lane).This demonstrates the CKB antibody detected the CKB protein (arrow).
TA328113 - CKBB
Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA328113) western blot analysis in MDA-MB-453,293 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the Creatine Kinase BB (CKB) antibody detected the Creatine Kinase BB (CKB) protein (arrow).
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CKBB antibody
  Rabbit Polyclonal antibody to Creatine kinase (brain) (creatine kinase, brain)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308722 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

CKBB antibody
  Goat Polyclonal Antibody against CKB / Brain Creatine Kinase  
TA302453 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

CKBB (N-term) antibody
The anti-CKB Pab is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

CKBB antibody
The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

4 Item(s)

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Product Type
  1. Primary Antibody (4)
Target
  1. CKBB (4)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (4)
  3. Canine (Can) (1)
  4. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (4)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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