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CSK antibody

Principal name

CSK antibody

Alternative names for CSK antibody

Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL

SwissProt ID

P32577 (Rat), P41239 (Chick), P41240 (Human), P41241 (Mouse), Q0VBZ0 (Bovin)

Gene ID

1445 (CSK), 315707 (Csk)

Ncbi ID

NP_001120662.1, 9606, NP_004374, NP_001120662, NM_004383, NP_004374.1, NM_001030039, NM_001127190

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CSK antibody

Kit Component:: - KN210758G1, CSK gRNA vector 1 in pCas-Guide vector
- KN210758G2, CSK gRNA vector 2 in pCas-Guide vector
- KN210758D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

1. Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.
2. Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
3. Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
4. Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
5. Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
6. Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J
Biol Chem. 2008 Apr 18;283(16):10339-46.

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

SM3138P

Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]

H00001445-M01

Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00001445-M01

Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.

H00001445-M01

CSK monoclonal antibody (M01), clone 3A3 Western Blot analysis of CSK expression in HL-60 ( Cat # L014V1 ).

H00001445-M01

CSK monoclonal antibody (M01), clone 3A3. Western Blot analysis of CSK expression in Hela NE ( Cat # L013V3 ).

H00001445-M01

Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.

H00001445-M01

CSK polyclonal antibody ( Cat # PAB6039 ) staining ( 0.1 µg/ml ) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A ) Strong staining of germinal centres. B ) High magnification view of germinal centre cells.

PAB6039

CSK polyclonal antibody ( Cat # PAB6039 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6039

Immunofluorescence staining of CSK in human HeLa cell line using CSK monoclonal antibody, clone CSK - 04 ( Cat # MAB0950 ; green ) . Actin cytoskeleton was decorated by phalloidin ( red ) and cell nuclei stained with DAPI ( blue ) .

MAB0950

Western Blot analysis of CSK expression in transfected 293T cell line (H00001445-T03) by CSK MaxPab polyclonal antibody.Lane 1: CSK transfected lysate(50.70 KDa).Lane 2: Non-transfected lysate.

H00001445-D01P

Immunohistochemistry analyzes of Csk antibody (AP20298PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20298PU-N

Western blot analyzes of Csk antibody (AP20298PU-N) in extracts from Raw264.7 cells.

AP20298PU-N

Western blot (WB) analysis of Csk antibody (Cat.-No.: AP20417PU-N) in extracts from HepG2 cells.

AP20417PU-N

Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK (Ab-364) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Raw264.7 cells, treated with UV 5', using CSK (Ab-364) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using CSK Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using CSK Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of U251 cells using CSK antibody Cat.-No AM06531SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

AM06531SU-N

Western blot analysis using CSK antibody Cat.-No AM06531SU-N against NIH/3T3 (1)，Hela (2)，COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.

AM06531SU-N

Flow cytometric analysis of HL-60 cells using CSK antibody Cat.-No AM06531SU-N (green) and negative control (purple).

AM06531SU-N

Western blot analysis of CSK (pS364) expression in HEK293T PMA-treated (A); NIH3T3 PMA-treated (B); H9C2 PMA-treated (C) whole cell lysates.

Western blot analysis of CSK expression in HepG2 (A); Raw264.7 (B); PC12 (C) whole cell lysates.

TP310758

TP310758

TP325740

TA302657 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302657

Western blot analysis of extracts from Jurkat cells, using CSK antibody.The lane on the right is treated with the synthesized peptide.

TA312151

Immunofluorescence analysis of A549 cells, using CSK antibody.The picture on the right is treated with the synthesized peptide.

TA312151

WB Suggested Anti-CSK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human heart

TA340049

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA302657	CSK (C-term) antibody
	Goat Polyclonal Antibody against CSK
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Rt	E, P, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA312151	CSK antibody
	Rabbit polyclonal anti-CSK antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA346925	CSK antibody
	Mouse Monoclonal CSK Antibody
	Mouse	IgG2b	2B4-C6-A10	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
AM06531SU-N	CSK antibody
	Mouse	IgG1	5F3	Ascites	Hu, Mky, Ms, Rt	E, F, ICC/IF, WB	0.1 ml / €380.00
							OriGene Technologies GmbH
+2 additional images
AP20298PU-N	CSK antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP20417PU-N	CSK antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
SM3138P	CSK antibody
	Mouse	IgG1	CSK-04	Purified	Hu, Ms	ICC/IF, IP, WB	0.1 ml / €260.00
							OriGene Technologies GmbH
SP1415P	CSK antibody
	Goat			Purified	Hu, Ms	WB	0.1 mg / €740.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP310758	CSK (transcript variant 1)
	Recombinant protein of human c-src tyrosine kinase (CSK), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.
+1 additional image
TP325740	CSK (transcript variant 2)
	Recombinant protein of human c-src tyrosine kinase (CSK), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR202254L2V	Lenti ORF particles, Csk (GFP-tagged ORF) - Rat c-src tyrosine kinase (Csk), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

RR202254L1V	Lenti ORF particles, Csk (Myc-DDK-tagged ORF) - Rat c-src tyrosine kinase (Csk), 200 uL, >10^7 TU/mL
		200 µl / €1,000.00
		OriGene Technologies, Inc.

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