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CSK antibody

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Principal name

CSK antibody

Alternative names for CSK antibody

Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL

SwissProt ID

P32577 (Rat), P41239 (Chick), P41240 (Human), P41241 (Mouse), Q0VBZ0 (Bovin)

Gene ID

1445 (CSK), 315707 (Csk)

Ncbi ID

NP_001120662.1, 9606, NP_004374, NP_001120662, NM_004383, NP_004374.1, NM_001030039, NM_001127190

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CSK antibody

Kit Component:
- KN210758G1, CSK gRNA vector 1 in pCas-Guide vector
- KN210758G2, CSK gRNA vector 2 in pCas-Guide vector
- KN210758D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.
2. Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
3. Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
4. Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
5. Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
6. Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J
Biol Chem. 2008 Apr 18;283(16):10339-46.

SM3138P - CSK
Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
H00001445-M01 - CSK
Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
H00001445-M01 - CSK
Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00001445-M01 - CSK
Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.
H00001445-M01 - CSK
CSK monoclonal antibody (M01), clone 3A3 Western Blot analysis of CSK expression in HL-60 ( Cat # L014V1 ).
H00001445-M01 - CSK
CSK monoclonal antibody (M01), clone 3A3. Western Blot analysis of CSK expression in Hela NE ( Cat # L013V3 ).
H00001445-M01 - CSK
Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.
PAB6039 - CSK
CSK polyclonal antibody ( Cat # PAB6039 ) staining ( 0.1 µg/ml ) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A ) Strong staining of germinal centres. B ) High magnification view of germinal centre cells.
PAB6039 - CSK
CSK polyclonal antibody ( Cat # PAB6039 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
MAB0950 - CSK
Immunofluorescence staining of CSK in human HeLa cell line using CSK monoclonal antibody, clone CSK - 04 ( Cat # MAB0950 ; green ) . Actin cytoskeleton was decorated by phalloidin ( red ) and cell nuclei stained with DAPI ( blue ) .
H00001445-D01P - CSK
Western Blot analysis of CSK expression in transfected 293T cell line (H00001445-T03) by CSK MaxPab polyclonal antibody.Lane 1: CSK transfected lysate(50.70 KDa).Lane 2: Non-transfected lysate.
AP20298PU-N - CSK
Immunohistochemistry analyzes of Csk antibody (AP20298PU-N) in paraffin-embedded human breast carcinoma tissue.
AP20298PU-N - CSK
Western blot analyzes of Csk antibody (AP20298PU-N) in extracts from Raw264.7 cells.
AP20417PU-N - CSK
Western blot (WB) analysis of Csk antibody (Cat.-No.: AP20417PU-N) in extracts from HepG2 cells.
A0415-1 - CSK
Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using Csk (Phospho-Ser364) Antibody. The lane on the right is treated with the synthesized peptide.
B0415-1 - CSK
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK (Ab-364) Antibody. The picture on the right is treated with the synthesized peptide.
B0415-1 - CSK
Western blot analysis of extracts from Raw264.7 cells, treated with UV 5', using CSK (Ab-364) Antibody. The lane on the right is treated with the synthesized peptide.
C10598-1 - CSK
Immunofluorescence analysis of A549 cells, using CSK Antibody. The picture on the right is treated with the synthesized peptide.
C10598-1 - CSK
Western blot analysis of extracts from Jurkat cells, using CSK Antibody. The lane on the right is treated with the synthesized peptide.
AM06531SU-N - CSK
Immunofluorescence analysis of U251 cells using CSK antibody Cat.-No AM06531SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06531SU-N - CSK
Western blot analysis using CSK antibody Cat.-No AM06531SU-N against NIH/3T3 (1)，Hela (2)，COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
AM06531SU-N - CSK
Flow cytometric analysis of HL-60 cells using CSK antibody Cat.-No AM06531SU-N (green) and negative control (purple).
AR51662PU-N - CSK
CPA1279-100ul - CSK
Western blot analysis of CSK (pS364) expression in HEK293T PMA-treated (A); NIH3T3 PMA-treated (B); H9C2 PMA-treated (C) whole cell lysates.
CPA3189-100ul - CSK
Western blot analysis of CSK expression in HepG2 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
TP310758 - CSK
TP310758 - CSK
TP325740 - CSK
TA302657 - CSK
TA302657 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA312151 - CSK
Western blot analysis of extracts from Jurkat cells, using CSK antibody.The lane on the right is treated with the synthesized peptide.
TA312151 - CSK
Immunofluorescence analysis of A549 cells, using CSK antibody.The picture on the right is treated with the synthesized peptide.
TA340049 - CSK
WB Suggested Anti-CSK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human heart

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CSK antibody
Immunofluorescence analysis of U251 cells using CSK antibody Cat.-No AM06531SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5F3 Ascites Hu, Mky, Ms, Rt E, F, ICC/IF, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
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  1. Primary Antibody (1)
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  2. Monkey (Mky) (1)
  3. Mouse (Ms) (1)
  4. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (1)
  2. Flow cytometry (F) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Western blot / Immunoblot (WB) (1)
Presentation
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  2. 3+ images (1)
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