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DLK1 antibody

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Principal name

DLK1 antibody

Alternative names for DLK1 antibody

DLK-1, DLK, Protein delta homolog 1, pG2, PREF1, Preadipocyte factor 1

SwissProt ID

P80370 (Human), Q09163 (Mouse)

Gene ID

8788 (DLK1), 13386 (Dlk1)

Ncbi ID

NP_003827.3, NP_003827, NP_034182.2, NM_010052, NM_001190703, NM_001190704, NM_001190705, NM_003836

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of DLK1 antibody

Delta like protein (Dlk), also known as Preadipocyte factor-1 (Pref-1) or zona glomerulosa-specific factor (ZOG), is an EGF-like transmembrane protein expressed preadipocytes but not in mature adipocytes. It is highly expressed in fetal liver, the adrenal gland, and placenta, as well as some neuroendocrine tumors and small cell lung carcinomas, where it plays a role in differentiation and proliferation. Dlk positively and negatively regulates adipocyte differentiation via at least four major variants (45-60 kDa) of Dlk generated by alternatively splicing. Constitutive expression of Dlk inhibits adipogenesis, but insulin or insulin like growth factor-1 (IGF-1) can circumvent this inhibition. Regulated processing of Dlk releases a 50 kDa soluble form that was previously characterized as Fetal Antigen-1, a protein involved in pancreatic island cell differentiation.

General readings

1. Yanai, H. et al., J. Biochem. 148(1): 85-92 (2010).
2. Yu, F. et al., Liver Int. 30(5): 703-714 (2010).
3. Akiyama, J. et al., Biochem. Biophys. Res. Commun. 393(4): 662-667 (2010).
4. Wermter, A.K. et al., Eur. J. Hum. Genet. 16(9): 1126-1134 (2008).
5. Charlier, C. et al., Genome Res. 11(5): 850-862 (2001).

H00008788-B01P - DLK1
Western Blot analysis of DLK1 expression in transfected 293T cell line (H00008788-T02) by DLK1 MaxPab polyclonal antibody.Lane 1: DLK1 transfected lysate(42.13 KDa).Lane 2: Non-transfected lysate.
H00008788-D01P - DLK1
DLK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of DLK1 expression in mouse liver.
H00008788-D01P - DLK1
Western Blot analysis of DLK1 expression in transfected 293T cell line (H00008788-T02) by DLK1 MaxPab polyclonal antibody.Lane 1: DLK1 transfected lysate(41.20 KDa).Lane 2: Non-transfected lysate.
ARP46375_P050 - DLK1
Human Stomach; WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach; DLK1 antibody - middle region (ARP46375_P050) in Human Stomach cells using Western Blot
AP51272PU-N - DLK1
Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using DLK1 antibody Cat.-No. AP51272PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLK1 antibody for IHC. Clinical relevance has not been evaluated.
AP51272PU-N - DLK1
Western blot analysis in CEM cell line lysates (35ug/lane) using DLK1 antibody Cat.-No. AP51272PU-N. This demonstrates the DLK1 antibody detected the DLK1 protein (arrow).
AM06690SU-N - DLK1
Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06690SU-N - DLK1
Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)
AM06690SU-N - DLK1
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
AM06690SU-N - DLK1
Flow cytometric analysis of NIH/3T3 cells using DLK1 mouse mAb (green) and negative control (red).
AM26511AF-N - DLK1
Immunohistochemical detection of Dlkon frozen section of mouse fetal liver (E14) with AM26511AF-N.This data was provided from Laboratory of Cell Growth and Differentiation, IMCB, The University of Tokyo.
AM26511AF-N - DLK1
Flow cytometric analysis of Dlk expression on Mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511AF-N to the cells.
AM26511AF-N - DLK1
Immunohistochemical detection of Dlkon paraffin embedded section of mouse fetal liver (E14.5) with AM26511AF-N.
AM26511FC-N - DLK1
lFlow Cytometric analysis of Dlkexpression on mouse fetal liver cells (E14) using isotype control (left) and AM26511FC-N.
AM26511RP-N - DLK1
Flow cytometric analysis of Dlk expression on mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511RP-N to the cells.
TP302923 - DLK1
TP720389 - DLK1
AR52000PU-N - DLK1
TA319760 - DLK1
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 ug/mL.
TA319760 - DLK1
Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 ug/mL.
TA319760 - DLK1
Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 ug/mL.
TA339381 - DLK1
WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach
  1. Quick-Filters:
  2. Highly Validated (1)

10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DLK1 antibody
Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 3A10 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €410.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

DLK1 (C-term) antibody
  Rabbit Polyclonal DLK1 Antibody  
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

DLK1 antibody
Immunohistochemical detection of Dlkon paraffin embedded section of mouse fetal liver (E14.5) with AM26511AF-N. Rat IgG1 24-11 Azide Free Ms C, F, P
0.1 mg / €400.00
  OriGene Technologies GmbH
+2 additional images

DLK1 antibody
lFlow Cytometric analysis of Dlkexpression on mouse fetal liver cells (E14) using isotype control (left) and AM26511FC-N. Rat IgG1 24-11 FITC Ms F
0.1 ml / €400.00
  OriGene Technologies GmbH

DLK1 antibody
Flow cytometric analysis of Dlk expression on mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511RP-N to the cells. Rat IgG1 24-11 PE Ms F
50 Tests / €430.00
  OriGene Technologies GmbH

DLK1 antibody
Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using DLK1 antibody Cat.-No. AP51272PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLK1 antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DLK1
  Recombinant protein of human delta-like 1 homolog (Drosophila) (DLK1)  
DLK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

DLK1
  Recombinant protein of human delta-like 1 homolog (Drosophila) (DLK1)  
DLK1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dlk1 (GFP-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dlk1 (GFP-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Dlk1 (Myc-DDK-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Dlk1 (Myc-DDK-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (6)
  3. Protein | Growth Factor (2)
Clonality
  1. Monoclonal (4)
  2. Polyclonal (2)
Target
  1. DLK1 (8)
Property
  1. C-term (1)
Reactivity
  1. Mouse (Ms) (4)
  2. Human (Hu) (3)
  3. Rat (Rt) (1)
Applications
  1. Flow cytometry (F) (4)
  2. Western blot / Immunoblot (WB) (3)
  3. Enzyme Immunoassay (E) (2)
  4. Paraffin Sections (P) (2)
  5. Frozen sections (C) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (2)
  2. Ascites (1)
  3. Azide Free (1)
  4. FITC (1)
  5. PE (1)
Host
  1. Rat (3)
  2. Rabbit (2)
  3. Mouse (1)
Images
  1. 1 image (8)
  2. 3+ images (3)
Images: Applications
  1. Flow cytometry (F) (4)
  2. Western blot / Immunoblot (WB) (3)
  3. Paraffin Sections (P) (2)
  4. Frozen sections (C) (1)
  5. Enzyme Immunoassay (E) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  7. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (10)
Service
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