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DLK1 antibody

Principal name

DLK1 antibody

Alternative names for DLK1 antibody

DLK-1, DLK, Protein delta homolog 1, pG2, PREF1, Preadipocyte factor 1

SwissProt ID

P80370 (Human), Q09163 (Mouse)

Gene ID

8788 (DLK1), 13386 (Dlk1)

Ncbi ID

NP_003827.3, NP_003827, NP_034182.2, NM_010052, NM_001190703, NM_001190704, NM_001190705, NM_003836

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of DLK1 antibody

Delta like protein (Dlk), also known as Preadipocyte factor-1 (Pref-1) or zona glomerulosa-specific factor (ZOG), is an EGF-like transmembrane protein expressed preadipocytes but not in mature adipocytes. It is highly expressed in fetal liver, the adrenal gland, and placenta, as well as some neuroendocrine tumors and small cell lung carcinomas, where it plays a role in differentiation and proliferation. Dlk positively and negatively regulates adipocyte differentiation via at least four major variants (45-60 kDa) of Dlk generated by alternatively splicing. Constitutive expression of Dlk inhibits adipogenesis, but insulin or insulin like growth factor-1 (IGF-1) can circumvent this inhibition. Regulated processing of Dlk releases a 50 kDa soluble form that was previously characterized as Fetal Antigen-1, a protein involved in pancreatic island cell differentiation.

General readings

1. Yanai, H. et al., J. Biochem. 148(1): 85-92 (2010).
2. Yu, F. et al., Liver Int. 30(5): 703-714 (2010).
3. Akiyama, J. et al., Biochem. Biophys. Res. Commun. 393(4): 662-667 (2010).
4. Wermter, A.K. et al., Eur. J. Hum. Genet. 16(9): 1126-1134 (2008).
5. Charlier, C. et al., Genome Res. 11(5): 850-862 (2001).

Western Blot analysis of DLK1 expression in transfected 293T cell line (H00008788-T02) by DLK1 MaxPab polyclonal antibody.Lane 1: DLK1 transfected lysate(42.13 KDa).Lane 2: Non-transfected lysate.

H00008788-B01P

DLK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of DLK1 expression in mouse liver.

H00008788-D01P

Western Blot analysis of DLK1 expression in transfected 293T cell line (H00008788-T02) by DLK1 MaxPab polyclonal antibody.Lane 1: DLK1 transfected lysate(41.20 KDa).Lane 2: Non-transfected lysate.

H00008788-D01P

Human Stomach; WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach; DLK1 antibody - middle region (ARP46375_P050) in Human Stomach cells using Western Blot

ARP46375_P050

Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using DLK1 antibody Cat.-No. AP51272PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLK1 antibody for IHC. Clinical relevance has not been evaluated.

AP51272PU-N

Western blot analysis in CEM cell line lysates (35ug/lane) using DLK1 antibody Cat.-No. AP51272PU-N. This demonstrates the DLK1 antibody detected the DLK1 protein (arrow).

AP51272PU-N

Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

AM06690SU-N

Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)

AM06690SU-N

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

AM06690SU-N

Flow cytometric analysis of NIH/3T3 cells using DLK1 mouse mAb (green) and negative control (red).

AM06690SU-N

Immunohistochemical detection of Dlkon frozen section of mouse fetal liver (E14) with AM26511AF-N.This data was provided from Laboratory of Cell Growth and Differentiation, IMCB, The University of Tokyo.

AM26511AF-N

Flow cytometric analysis of Dlk expression on Mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511AF-N to the cells.

AM26511AF-N

Immunohistochemical detection of Dlkon paraffin embedded section of mouse fetal liver (E14.5) with AM26511AF-N.

AM26511AF-N

lFlow Cytometric analysis of Dlkexpression on mouse fetal liver cells (E14) using isotype control (left) and AM26511FC-N.

AM26511FC-N

Flow cytometric analysis of Dlk expression on mouse Fetal Hepatocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26511RP-N to the cells.

AM26511RP-N

TP302923

TP720389

Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 ug/mL.

TA319760

Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 ug/mL.

TA319760

Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 ug/mL.

TA319760

WB Suggested Anti-DLK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach

TA339381

Mouse Lenti ORF Particles

Catalog No.
MR206011L2V	Lenti ORF particles, Dlk1 (GFP-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR206011L1V	Lenti ORF particles, Dlk1 (Myc-DDK-tagged) - Mouse delta-like 1 homolog (Drosophila) (Dlk1), transcript variant 1, 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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