DMRT1 antibody
Principal name
DMRT1 antibody
Alternative names for DMRT1 antibody
Doublesex- and mab-3-related transcription factor 1, DMT1, DM domain expressed in testis protein 1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of DMRT1 antibody
The gene encodes the DMRT1 protein is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. This suggested that DMRT1 may be required for testis development.This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Cheng,H.H., (2006) Cell Res. 16 (4), 389-393
DMRT1 monoclonal antibody (M01), clone 1G11 Western Blot analysis of DMRT1 expression in HL-60 ( Cat # L014V1 ).
Western Blot analysis of DMRT1 expression in transfected 293T cell line by DMRT1 monoclonal antibody (M01), clone 1G11.Lane 1: DMRT1 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged DMRT1 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
Human Brain; WB Suggested Anti-DMRT1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; DMRT1 antibody - N-terminal region (P100722_P050) in Human Brain cells using Western Blot
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(39.50 KDa).Lane 2: Non-transfected lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-DMRT1 monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807539)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807669)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807746)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
WB Suggested Anti-DMRT1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
WB Suggested Anti-Dmrt1 Antibody Titration: 0.2-1 ug/ml; Positive Control: NIH/3T3 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807504 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI3G10 (formerly 3G10) | |||||||
 |
Mouse | IgG1 | OTI3G10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807517 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
 |
Mouse | IgG2b | OTI1G10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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| TA807522 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI5D6 (formerly 5D6) | |||||||
 |
Mouse | IgG1 | OTI5D6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807523 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI2A10 (formerly 2A10) | |||||||
 |
Mouse | IgG2a | OTI2A10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807539 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5) | |||||||
 |
Mouse | IgG2b | OTI4B5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA807669 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12) | |||||||
 |
Mouse | IgG2b | OTI4E12 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA807746 | DMRT1 antibody |
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| DMRT1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG1 | OTI2H1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308108 | DMRT1 |
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| Recombinant protein of human doublesex and mab-3 related transcription factor 1 (DMRT1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411862 | DMRT1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
