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DMRT1 antibody

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Principal name

DMRT1 antibody

Alternative names for DMRT1 antibody

Doublesex- and mab-3-related transcription factor 1, DMT1, DM domain expressed in testis protein 1

SwissProt ID

Q3LH63 (Xenla), Q9PTQ7 (Chick), Q9QZ59 (Mouse), Q9TT01 (Pig), Q9Y5R6 (Human)

Gene ID

1761 (DMRT1), 50796 (Dmrt1)

Ncbi ID

9606, NP_068770, NP_068770.2, NM_021951, NM_015826

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Por (Porcine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of DMRT1 antibody

The gene encodes the DMRT1 protein is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. This suggested that DMRT1 may be required for testis development.This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Cheng,H.H., (2006) Cell Res. 16 (4), 389-393

H00001761-M01 - DMRT1
DMRT1 monoclonal antibody (M01), clone 1G11 Western Blot analysis of DMRT1 expression in HL-60 ( Cat # L014V1 ).
H00001761-M01 - DMRT1
Western Blot analysis of DMRT1 expression in transfected 293T cell line by DMRT1 monoclonal antibody (M01), clone 1G11.Lane 1: DMRT1 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.
H00001761-M01 - DMRT1
Detection limit for recombinant GST tagged DMRT1 is approximately 0.3ng/ml as a capture antibody.
H00001761-B01 - DMRT1
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
H00001761-B01P - DMRT1
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
P100722_P050 - DMRT1
Human Brain; WB Suggested Anti-DMRT1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; DMRT1 antibody - N-terminal region (P100722_P050) in Human Brain cells using Western Blot
H00001761-D01P - DMRT1
Western Blot analysis of DMRT1 expression in transfected 293T cell line (H00001761-T01) by DMRT1 MaxPab polyclonal antibody.Lane 1: DMRT1 transfected lysate(39.50 KDa).Lane 2: Non-transfected lysate.
TA807504 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.
TA807517 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.
TA807517 - DMRT1
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-DMRT1 monoclonal antibody at 1:200 dilution.
TA807522 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
TA807523 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:500)
TA807539 - DMRT1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807539)(1:150)
TA807539 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
TA807669 - DMRT1
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807669)(1:150)
TA807669 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
TA807746 - DMRT1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807746)(1:150)
TA807746 - DMRT1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1.(1:2000)
TP308108 - DMRT1
TA330051 - DMRT1
WB Suggested Anti-DMRT1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
TA342315 - DMRT1
WB Suggested Anti-Dmrt1 Antibody Titration: 0.2-1 ug/ml; Positive Control: NIH/3T3 cell lysate

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DMRT1 antibody
  DMRT1 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807539)(1:150) Mouse IgG2b OTI4B5 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

DMRT1 antibody
  DMRT1 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807669)(1:150) Mouse IgG2b OTI4E12 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

DMRT1 antibody
  DMRT1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807746)(1:150) Mouse IgG1 OTI2H1 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

3 Item(s)

per page

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Product Type
  1. Primary Antibody (3)
Clonality
  1. Monoclonal (3)
Target
  1. DMRT1 (3)
Reactivity
  1. Human (Hu) (3)
Applications
  1. Paraffin Sections (P) (3)
  2. Western blot / Immunoblot (WB) (3)
Presentation
  1. Purified (3)
Host
  1. Mouse (3)
Images
  1. 1 image (3)
Manufacturer
  1. OriGene Technologies (3)
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