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EGF antibody

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Principal name

EGF antibody

Alternative names for EGF antibody

Urogastrone, Epidermal growth factor, URG, HOMG4, Pro-epidermal growth factor

SwissProt ID

P01132 (Mouse), P01133 (Human), P07522 (Rat), Q00968 (Pig)

Gene ID

1950 (EGF), 25313 (Egf), 13645

Ncbi ID

NP_001954, NP_001954.2, NP_036974, 10090, 9606, NP_034243, NP_036974.1, NM_001963, BC017681, NM_012842, NM_010113

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), ELISA (capture) (E(capture)), Paraffin Sections (P), Functional assay (FN), Neutralisation (NEUT), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot)

Background of EGF antibody

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF., amphiregulin (AR), betacellulin (BTC), epiregulin(EPR), heparinbinding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1 through-6. Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues . All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region . EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid . Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members . These receptors undergo a complex pattern of ligand induced homoor heterodimerization to transducer EGF family signals . EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules . Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

General readings

Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.

Carpenter, G. and S. Cohen, (1990) J. Biol. Chem. 265:7709.

Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.

Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res.164:1.

Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.

Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.

Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.

Qian, X. et al. (1999) J. Biol. Chem. 274:574.

AM09149PU-N - EGF
Staining of methanol-fixed Hela cells by Immunocytochemistry using Human EGF Antibody (Clone S-146) at 1/100 (10 µg/ml).
AM09149PU-N - EGF
The antibody recognized recombinant EGF on western blot.
AP02293SU-N - EGF Precursor (Suberites domuncula)
Western Blot analysis of AP02293SU specificity: Crude extract from S. domuncula tissue was separated by SDS-PAGE and immunoblotted with AP02293SU (1/500).
Lane a: Crude extract stained with Coomassie Brilliant Blue.
Lane b: AP02293SU detects a 5 kDa band which corresponds to a processed form of EGF from the 78 kDa precursor.
Lane d: AP02293SU preabsorbed with recombinant EGF precursor. Perovic-Ottstadt S et al. Eur J Biochem. 271:1924-1937.
AP20662PU-N - EGF
Immunohistochemistry (IHC) analysis of EGF antibody (Cat.-No.: AP20662PU-N) in paraffin-embedded human brain tissue.
AP20662PU-N - EGF
Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF antibody (Cat.-No.: AP20662PU-N)
AP51384PU-N - EGF
EGF Antibody (Center) (Cat. #AP51384PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EGF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
AP51384PU-N - EGF
EGF Antibody (Center) (Cat. #AP51384PU-N) western blot analysis in mouse stomach tissue lysates (35µg/lane).This demonstrates the EGF antibody detected the EGF protein (arrow).
ARP41486_P050 - EGF
Human COLO205; WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; EGF antibody - middle region (ARP41486_P050) in Human COLO205 cells using Western Blot
PAB9513 - EGF
Western blot analysis of EGF polyclonal antibody expression in recombinant human EGF.Lane 1 : 10 ng.Lane 2 : 50 ng.
PAB9514 - EGF
Western blot analysis of Egf polyclonal antibody expression in recombinant mouse Egf ( 50 ng/lane ) .
PP1002P1 - EGF
Immunohistochemistry: This EGF antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 µg/ml – 0.25 µg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.
PP1002P1 - EGF
AM06394SU-N - EGF
Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb
AM06394SU-N - EGF
Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung breast tissues using EGF mouse mAb
AM06394SU-N - EGF
Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HEK293 cell lysate.
AM06419SU-N - EGF
Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06419SU-N - EGF
Western blot analysis using EGF mAb against EGF(AA: 971-1023)-hIgGFc transfected HEK293 cell lysate.
AM26658SU-N - EGF

Immunoprecipitation of human EGF-R from A431 cells with 2 μg of AM26658SU-N (1), 10μg of AM26658SU-N (2) and mouse IgG (3). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26658SU-N.


AR50004PU-N - EGF
AR50356PU-N - EGF
AR31147PU-N - EGF
EGF-induced proliferation of NHDF cells (Normal Human Dermal Fibroblast cells).
AR31147PU-N - EGF
SDS-PAGE analysis of recombinant human EGF. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.
AR31147PU-N - EGF
EGF-induced phosphorylation of EGFR in the A431 tumor cell line (Soler et al, J Chromatography B, 788,113, 2003).
AR31156PU-N - EGF
SDS-PAGE analysis of recombinant human EGF. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.
AR31156PU-N - EGF
EGF-induced phosphorylation of EGFR in the A431 tumor cell line (Soler et al, J Chromatography B, 788,113, 2003).
AR31156PU-N - EGF
EGF-induced proliferation of NHDF cells (Normal Human Dermal Fibroblast cells).
CEK1140 - Human EGF ELISA Kit
Representative Standard Curve
CEK1141 - Human EGF ELISA Kit
Representative Standard Curve
CEK1455 - Mouse EGF ELISA Kit
Representative Standard Curve
CEK1456 - Mouse EGF ELISA Kit
Representative Standard Curve
CEK1594 - Rat EGF ELISA Kit
Representative Standard Curve
TA590718 - EGF
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGF (RC210817, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGF.
TA590718 - EGF
ELISA: EGF Antibody
TA590718 - EGF
TA590718 - EGF
TP310817 - EGF
TP723068 - EGF
TP723069 - EGF
TP723070 - EGF
TA324063 - EGF
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EGF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200)
TA324064 - EGF
Western blot analysis of Mouse liver tissue using TA324064(EGF Antibody) at dilution 1/500
TA324064 - EGF
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA324064(EGF Antibody)(left image) at dilution 1/25, the image on the right is treated with the fusion protein.(Original magnification: x200)
TA328291 - EGF
To detect hEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF.
TA328291 - EGF
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EGF (TA328292) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF.
TA328292 - EGF
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.
TA328292 - EGF
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.
TA328292 - EGF
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.
TA328292 - EGF
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.
TA328508 - EGF
To detect mEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
TA328508 - EGF
To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine EGF (TA328509) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
TA328509 - EGF
To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (TA328508) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF.
TA328509 - EGF
To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (TA328508) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF.
TA328577 - EGF
To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
TA328577 - EGF
To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
TA329069 - EGF
TA329069 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA346085 - EGF
WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysate
TA347892 - EGF
Western blot analysis of EGF expression in NIH/3T3 cells
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGF antibody
  Anti-EGF Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EGF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

EGF antibody
  Anti-EGF Rabbit Polyclonal Antibody  
Western blot analysis of Mouse liver tissue using TA324064(EGF Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

EGF antibody
  Biotinylated Anti-Human EGF Rabbit Polyclonal Antibody  
To detect hEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, WB
50 µg / €275.00
  OriGene Technologies, Inc.
+1 additional image

EGF antibody
  Anti-Human EGF Rabbit Polyclonal Antibody  
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+3 additional images

EGF antibody
  Biotinylated Anti-Murine EGF Goat Polyclonal Antibody  
To detect mEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
50 µg / €275.00
  OriGene Technologies, Inc.
+1 additional image

EGF antibody
  Anti-Murine EGF Goat Polyclonal Antibody  
To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (TA328508) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+1 additional image

EGF antibody
  Anti-Rat EGF Rabbit Polyclonal Antibody  
To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+1 additional image

EGF antibody
  Pro-EGF Goat Polyclonal Antibody  
TA329069 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

EGF antibody
  Rabbit Polyclonal Anti-EGF Antibody  
Western blot analysis of EGF expression in NIH/3T3 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

EGF antibody
Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb Mouse IgG1 9D7F11 Ascites Hu E, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+2 additional images

EGF antibody
Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4E11 Ascites Hu E, ICC/IF, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

EGF antibody
EGF Mouse IgG1 S-21 Aff - Purified Hu E
0.5 mg / €520.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG1 S-120 Aff - Purified Hu E
0.5 mg / €520.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG1 S-145 Aff - Purified Hu E
0.5 mg / €520.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG1 S-146 Biotin Hu E, E(detection)
0.1 ml / €570.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG1 S-146 HRP Hu E
0.2 ml / €570.00
  OriGene Technologies GmbH

EGF antibody
Staining of methanol-fixed Hela cells by Immunocytochemistry using Human EGF Antibody (Clone S-146) at 1/100 (10 µg/ml). Mouse IgG1 S-146 Purified Hu E(detection), ICC/IF, WB
0.5 mg / €520.00
  OriGene Technologies GmbH
+1 additional image

EGF antibody
EGF Mouse IgG1 S-147 Aff - Purified Hu E
0.5 mg / €520.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG1 S-177 Aff - Purified Hu E
0.5 mg / €520.00
  OriGene Technologies GmbH

EGF antibody
EGF Mouse IgG2a KT2 Aff - Purified Hu E
1 mg / €1,240.00
  OriGene Technologies GmbH

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