EIF2A antibody
Principal name
EIF2A antibody
Alternative names for EIF2A antibody
eIF-2A, Eukaryotic translation initiation factor 2A, CDA02, MSTP004, MSTP089, 65 kDa eukaryotic translation initiation factor 2A
Ncbi ID
9606, NP_114414, NP_004085, AAH11885.1, NP_114414.2, BC030447, NM_001005509, NM_032025
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)
Background of EIF2A antibody
eIF2α (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kise under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF2α has been linked to progression of thyroid carcinoma.Sigling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B α, eIF2B δ, eIF2B β subunit 2, p67, p68, and PKR. eIF2α is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10. The Poly6067 antibody recognizes both mouse and human eIF2α and has been shown to be useful for Western blotting.
General readings
Su,Q., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (1), 63-68
Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody (M01), clone 3D5.Lane 1: EIF2A transfected lysate(65 KDa).Lane 2: Non-transfected lysate.
Human Skin; Rabbit Anti-EIF2A Antibody. Catalog Number: ARP41041. Paraffin Embedded Tissue: Human Skin. Cellular Data: Epidermal cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EIF2A antibody - C-terminal region (ARP41041_T100) in Human Skin cells using Immunohistochemistry
Human Thymus; WB Suggested Anti-EIF2A Antibody Titration: 2.5ug/ml. Positive Control: Human Thymus; EIF2A antibody - C-terminal region (ARP41041_T100) in Human Thymus cells using Western Blot
AP08404PU-N EIF2A antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 1/50 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of EIF2A Antibody (Center) (Cat. #AP51390PU-N) in Ramos cell line lysates (35µg/lane). EIF2A (arrow) was detected using the purified Pab.
EIF2A Antibody (Center) (Cat. #AP51390PU-N) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA309039 diluted at 1:500.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Western Blot: eIF2A Antibody (3A7B11) [TA337129] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.
Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [TA337129] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
WB Suggested Anti-EIF2A Antibody Titration: 2.5 ug/ml; Positive Control: Human Thymus
Rabbit Anti-EIF2A Antibody; Paraffin Embedded Tissue: Human Skin; Cellular Data: Epidermal cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2alpha; antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309039 | EIF2A antibody |
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| Rabbit polyclonal antibody to EIF2A (eukaryotic translation initiation factor 2A, 65kDa) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| TA328041 | EIF2A antibody |
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| Rabbit Polyclonal anti-eIF2a Antibody | |||||||
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Rabbit | IgG | Poly6067 | Purified | Hu, Ms | WB |
0.2 ml /
€255.00
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| OriGene Technologies, Inc. | |||||||
| TA337129 | EIF2A antibody |
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| Mouse Monoclonal eIF2A Antibody (3A7B11) | |||||||
![Western Blot: eIF2A Antibody (3A7B11) [TA337129] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-26296-1-W.jpg) |
Mouse | IgG1 | 3A7B11 | Purified | Hu | C, F, WB |
0.1 ml /
€395.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP51390PU-N | EIF2A (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304303 | EIF2A |
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| Recombinant protein of human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207798L2V | Lenti ORF particles, Eif2a (GFP-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR209105L2V | Lenti ORF particles, Eif2a (GFP-tagged) - Mouse eukaryotic translation initiation factor 2A (Eif2a), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,310.00
|
|
| OriGene Technologies, Inc. | ||
| MR207798L1V | Lenti ORF particles, Eif2a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2a (cDNA clone MGC:40963 IMAGE:5400637), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR209105L1V | Lenti ORF particles, Eif2a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2A (Eif2a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||