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EIF2A antibody

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Principal name

EIF2A antibody

Alternative names for EIF2A antibody

eIF-2A, Eukaryotic translation initiation factor 2A, CDA02, MSTP004, MSTP089, 65 kDa eukaryotic translation initiation factor 2A

SwissProt ID

Q5ZKC1 (Chick), Q7ZY11 (Xenla), Q8BJW6 (Mouse), Q9BY44 (Human)

Gene ID

83939 (EIF2A), 229317 (Eif2a)

Ncbi ID

9606, NP_114414, NP_004085, AAH11885.1, NP_114414.2, BC030447, NM_001005509, NM_032025

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of EIF2A antibody

eIF2α (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kise under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF2α has been linked to progression of thyroid carcinoma.Sigling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B α, eIF2B δ, eIF2B β subunit 2, p67, p68, and PKR. eIF2α is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10. The Poly6067 antibody recognizes both mouse and human eIF2α and has been shown to be useful for Western blotting.

General readings

Su,Q., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (1), 63-68

H00083939-M01 - EIF2A
Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody (M01), clone 3D5.Lane 1: EIF2A transfected lysate(65 KDa).Lane 2: Non-transfected lysate.
ARP41041_T100 - EIF2A
Human Skin; Rabbit Anti-EIF2A Antibody. Catalog Number: ARP41041. Paraffin Embedded Tissue: Human Skin. Cellular Data: Epidermal cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EIF2A antibody - C-terminal region (ARP41041_T100) in Human Skin cells using Immunohistochemistry
ARP41041_T100 - EIF2A
Human Thymus; WB Suggested Anti-EIF2A Antibody Titration: 2.5ug/ml. Positive Control: Human Thymus; EIF2A antibody - C-terminal region (ARP41041_T100) in Human Thymus cells using Western Blot
AP08404PU-N - EIF2A
AP08404PU-N EIF2A antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 1/50 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
AP51390PU-N - EIF2A
Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP51390PU-N - EIF2A
Western blot analysis of EIF2A Antibody (Center) (Cat. #AP51390PU-N) in Ramos cell line lysates (35µg/lane). EIF2A (arrow) was detected using the purified Pab.
AP51390PU-N - EIF2A
EIF2A Antibody (Center) (Cat. #AP51390PU-N) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TP304303 - EIF2A
TA309039 - EIF2A
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA309039 diluted at 1:500.
TA328041 - EIF2A
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
TA337129 - EIF2A
Western Blot: eIF2A Antibody (3A7B11) [TA337129] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.
TA337129 - EIF2A
Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [TA337129] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
TA343946 - EIF2A
WB Suggested Anti-EIF2A Antibody Titration: 2.5 ug/ml; Positive Control: Human Thymus
TA343946 - EIF2A
Rabbit Anti-EIF2A Antibody; Paraffin Embedded Tissue: Human Skin; Cellular Data: Epidermal cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
TA352610 - EIF2A
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2alpha; antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF2A antibody
  Rabbit Polyclonal anti-eIF2a Antibody  
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6067 Purified Hu, Ms WB
0.2 ml / €255.00
  OriGene Technologies, Inc.

EIF2A antibody
  Mouse Monoclonal eIF2A Antibody (3A7B11)  
Western Blot: eIF2A Antibody (3A7B11) [TA337129] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml. Mouse IgG1 3A7B11 Purified Hu C, F, WB
0.1 ml / €395.00
  OriGene Technologies, Inc.
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Product Type
  1. Primary Antibody (2)
Clonality
  1. Monoclonal (1)
  2. Polyclonal (1)
Target
  1. EIF2A (2)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Frozen sections (C) (1)
  3. Flow cytometry (F) (1)
Host
  1. Mouse (1)
  2. Rabbit (1)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Immunofluorescence (IF) (1)
Manufacturer
  1. OriGene Technologies (2)
Service
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