ERCC1 antibody
Principal name
ERCC1 antibody
Alternative names for ERCC1 antibody
DNA excision repair protein ERCC-1, COFS4, UV20
Ncbi ID
NP_001974.1, 9606, NP_001974, NP_001159521, NP_973730.1, NM_202001, NP_973730, NM_007948, NM_001983, NM_001166049
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ERCC1 antibody
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of D lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the D lesion. The heterodimeric endonuclease is also involved in recombitiol D repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009].
General readings
Zhang N, Liu X, Li L, Legerski R. Double-strand breaks induce homologous recombinational repair of interstrand crosslinks via cooperation of MSH2, ERCC1-XPF, REV3, and the Fanconi anemia pathway. DNA Repair (Amst). 2007 Jul 30. PMID: 17669695
Detection limit for recombinant GST tagged ERCC1 is approximately 0.03ng/ml as a capture antibody.
Immunohistochemistry (IHC) analysis of ERCC1 antibody (Cat.-No.: AP06106PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of ERCC1 antibody (Cat.-No.: AP06106PU-N) at 1/500 dilution
Lane1: MCF-7 whole cell lysate
Lane2: Mouse liver tissue lysate
Lane3: Rat kidney tissue lysate
Lane1: MCF-7 whole cell lysate
Lane2: Mouse liver tissue lysate
Lane3: Rat kidney tissue lysate
Immunohistochemical staining using ERCC1 antibody on Formalin Fixed, Paraffin Embedded Human tonsil tissues.
Western Blot analysis of ERCC1 expression in transfected 293T cell line (H00002067-T02) by ERCC1 MaxPab polyclonal antibody.Lane 1: ERCC1 transfected lysate(35.60 KDa).Lane 2: Non-transfected lysate.
AP09588PU ERCC1 antibody staining of Human Lung SCC.
AP09588PU ERCC1 antibody staining of HeLa cell lysate by Western Blot analysis.
Human NSCLC stained with anti-ERCC1 antibody (Left +, Right -)
AP23054PU-N ERCC1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil.
Immunohistochemical analysis of ERCC1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ERCC1 expression in MCF7 (A); HeLa (B); A431 (C); mouse liver (D); rat kidney (E) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500622, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA500622) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1 (RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1 .
HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA500622, Dilution 1:1,000), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501140, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501140), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501167, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501167, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501167, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501167, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501167) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA501167),(Red), compared to a nonspecific negative control antibody,(Blue) at 1:100
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501167), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501168, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501168, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501168) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501168), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501169, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501169, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501169, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501169, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody at 1:150 (TA501169)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody at 1:150 (TA501169)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501169, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501169) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501169), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA501169),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA501169),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-ERCC1 mouse monoclonal antibody (TA501181) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501181), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501182, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501182, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501182, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501182, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501182) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501182), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501183, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501183, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501183, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501183), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA501184) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501184), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA501184),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501185, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501185, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501185, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501185, Dilution 1:50)
Anti-ERCC1 mouse monoclonal antibody (TA501185) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501185), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501695), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA501726) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501726), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ERCC1 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501766), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501767), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA501828), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA504286) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504286), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA504286),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-ERCC1 mouse monoclonal antibody (TA504287) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504287), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504288), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA504288),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-ERCC1 mouse monoclonal antibody (TA504333) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504333), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA504347) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504347), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA504365) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504365), and then analyzed by flow cytometry.
Anti-ERCC1 mouse monoclonal antibody (TA504367) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504367), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504405)
Anti-ERCC1 mouse monoclonal antibody (TA504405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504405), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805607)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805666)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805668)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805668)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805668)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805668)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805691)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805691)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 4F9). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 2E12). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.
TA303306 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ERCC1 Antibody (C-term) (Cat. #TA324711) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).
Western blot analysis of extracts from NIH/3T3 cells, using ERCC1 antibody (#TA326228).The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Hela cells (Lane 2), using ERCC1 Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ERCC1 antibody (#TA326228).The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using ERCC1 antibody.
Immunohistochemistry of paraffin-embedded human kidney using ERCC1 antibody at dilution of 1:100 (400x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using ERCC1 antibody at dilution of 1:100 (400x lens).
Immunofluorescence analysis of U2OS cell using ERCC1 antibody. Blue: DAPI for nuclear staining.
Western blot analysis on NIH-3T3 cell lysate using ERCC1 Antibody
Western blot analysis of ERCC1 expression in Hela (A); HepG2 (B); 293T (C); Jurkat (D) whole cell lysates.
Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500008 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone 4F9 | |||||||
 |
Mouse | IgG1 | 4F9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +14 additional images |
Published recently! Highly validated antibody!
|
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| UM570008 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone 4F9 | |||||||
|
Mouse | IgG1 | 4F9 | Purified | Hu | ICC/IF, P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Published recently! Highly validated antibody!
|
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| UM500011 | ERCC1 antibody |
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| ERCC1 mouse monoclonal antibody, clone 2E12 | |||||||
 |
Mouse | IgG2b | 2E12 | Purified | Hu, Mky, Rt | F, ICC/IF, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +18 additional images |
Highly validated antibody!
|
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| UM570011 | ERCC1 antibody |
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| ERCC1 mouse monoclonal antibody, clone 2E12 | |||||||
|
Mouse | IgG2b | 2E12 | Purified | Hu, Mky, Rt | F, ICC/IF, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +18 additional images |
Highly validated antibody!
|
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| TA332586 | ERCC1 antibody |
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| Rabbit anti-ERCC1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA500622 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3) | |||||||
 |
Mouse | IgG1 | OTI1A3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
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| TA501140 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) | |||||||
 |
Mouse | IgG1 | OTI2H5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA501167 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI1B3 (formerly 1B3) | |||||||
 |
Mouse | IgG1 | OTI1B3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA501168 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI3F2 (formerly 3F2) | |||||||
 |
Mouse | IgG1 | OTI3F2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA501169 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
 |
Mouse | IgG1 | OTI1C6 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA501182 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) | |||||||
 |
Mouse | IgG1 | OTI2A7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA501183 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11) | |||||||
 |
Mouse | IgG1 | OTI2E11 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA501184 | ERCC1 antibody |
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| ERCC1 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1) | |||||||
 |
Mouse | IgG2a | OTI1H1 | Purified | Can, Hu, Mky | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA501185 | ERCC1 antibody |
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| anti-ERCC1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6) | |||||||
 |
Mouse | IgG1 | OTI1G6 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA501726 | ERCC1 antibody |
||||||
| anti-ERCC1 mouse monoclonal antibody, clone OTI2A9 (formerly 2A9) | |||||||
 |
Mouse | IgG2b | OTI2A9 | Purified | Can, Hu, Mky | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA504286 | ERCC1 antibody |
||||||
| ERCC1 mouse monoclonal antibody, clone OTI24F2 (formerly 24F2) | |||||||
 |
Mouse | IgG1 | OTI24F2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA504365 | ERCC1 antibody |
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| ERCC1 mouse monoclonal antibody, clone OTI1C10 (formerly 1C10) | |||||||
 |
Mouse | IgG2a | OTI1C10 | Purified | Hu, Ms | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA504367 | ERCC1 antibody |
||||||
| ERCC1 mouse monoclonal antibody, clone OTI3B1 (formerly 3B1) | |||||||
 |
Mouse | IgG2a | OTI3B1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA504405 | ERCC1 antibody |
||||||
| ERCC1 mouse monoclonal antibody, clone OTI5A2 (formerly 5A2) | |||||||
 |
Mouse | IgG1 | OTI5A2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA805607 | ERCC1 antibody |
||||||
| ERCC1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1) | |||||||
 |
Mouse | IgG1 | OTI2H1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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