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FANCF antibody

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Principal name

FANCF antibody

Alternative names for FANCF antibody

Fanconi anemia group F protein, FACF

SwissProt ID

Q9NPI8 (Human)

Gene ID

2188 (FANCF)

Ncbi ID

NP_073562.1, 9606, NP_073562, NM_022725

Available reactivities

Hu (Human), Mky (Monkey), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FANCF antibody

FANCF (also called Protein FACF or Fanconi Anemia Group F protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCF has also been implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCF belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. FANCF is found within the nucleus. Defects in FANCF are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level, it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

AP07528PU-N - FANCF
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
H00002188-B01 - FANCF
Western Blot analysis of FANCF expression in transfected 293T cell line (H00002188-T01) by FANCF MaxPab polyclonal antibody.Lane 1: FANCF transfected lysate(41.14 KDa).Lane 2: Non-transfected lysate.
PAB10011 - FANCF
Western blot using FANCF polyclonal antibody ( Cat # PAB10011 ) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA ( lanes 3 and 4 ).This band ( indicated by arrowhead ) is approximately 42.3 kDa in size.The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein ( lanes 1 and 2 ).Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.No apparent difference was noted upon irradiation.The strong band at ~60kDa appears to be non-specific.Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
AP09249PU-N - FANCF
Western blot using anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
AP22410PU-N - FANCF
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with AP22410PU-N 1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22410PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
TA311512 - FANCF
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with TA311512 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311512 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
TA322193 - FANCF
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using FANCF antibody at dilution 1/100, on the right is treated with the fusion protein. (Original magnification: x200)

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCF (217-228) antibody
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with AP22410PU-N 1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22410PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

FANCF (C-term) antibody
  Goat Anti-FANCF Antibody  
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with TA311512 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311512 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

FANCF antibody
  Anti-FANCF Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using FANCF antibody at dilution 1/100, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

FANCF (Internal) antibody
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

FANCF antibody
Western blot using anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €515.00
  OriGene Technologies GmbH

5 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (5)
Clonality
  1. Polyclonal (5)
Target
  1. FANCF (5)
Property
  1. 217-228 (1)
  2. C-term (1)
  3. Internal (1)
Reactivity
  1. Human (Hu) (5)
  2. Chimpanzee (Chimp) (1)
  3. Mouse (Ms) (1)
  4. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (5)
  2. Western blot / Immunoblot (WB) (4)
  3. Paraffin Sections (P) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (4)
  2. Purified (1)
Host
  1. Rabbit (3)
  2. Goat (2)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
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