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FANCF antibody

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Principal name

FANCF antibody

Alternative names for FANCF antibody

Fanconi anemia group F protein, FACF

SwissProt ID

Q9NPI8 (Human)

Gene ID

2188 (FANCF)

Ncbi ID

NP_073562.1, 9606, NP_073562, NM_022725

Available reactivities

Hu (Human), Mky (Monkey), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FANCF antibody

FANCF (also called Protein FACF or Fanconi Anemia Group F protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCF has also been implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCF belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. FANCF is found within the nucleus. Defects in FANCF are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level, it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

AP07528PU-N - FANCF
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
H00002188-B01 - FANCF
Western Blot analysis of FANCF expression in transfected 293T cell line (H00002188-T01) by FANCF MaxPab polyclonal antibody.Lane 1: FANCF transfected lysate(41.14 KDa).Lane 2: Non-transfected lysate.
PAB10011 - FANCF
Western blot using FANCF polyclonal antibody ( Cat # PAB10011 ) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA ( lanes 3 and 4 ).This band ( indicated by arrowhead ) is approximately 42.3 kDa in size.The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein ( lanes 1 and 2 ).Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.No apparent difference was noted upon irradiation.The strong band at ~60kDa appears to be non-specific.Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
AP09249PU-N - FANCF
Western blot using anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
AP22410PU-N - FANCF
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with AP22410PU-N 1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22410PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
TA311512 - FANCF
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with TA311512 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311512 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
TA322193 - FANCF
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using FANCF antibody at dilution 1/100, on the right is treated with the fusion protein. (Original magnification: x200)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCF (Internal) antibody
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

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  1. Enzyme Immunoassay (E) (1)
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