GPR75 antibody
Principal name
GPR75 antibody
Alternative names for GPR75 antibody
Orphan retinal GPCR, G-protein coupled receptor 75
Ncbi ID
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of GPR75 antibody
Recently a novel human G-protein coupled receptor gene has been characterized and mapped to chromosome 2p16. This gene codes for a 540 amino acid protein in retinal pigment epithelium (RPE) and cells surrounding retinal arterioles. In contrast, the Northern blot data obtained from mouse sections suggest the expression of transcripts in photoreceptor inner segments and I outer plexiform layer. The transcripts of the GPCR-75 gene (7kb) are also found in abundance in brain sections. So far, no mutations in GPCR-75 protein were identified in patients suffering from Doyne’s honeycomb retinal dystrophy (DHRD), an inherited retinal degeneration disease that maps to chromosome 2p16 (1).
The GPCR-75 protein is approximately 78 kDa (540 amino acids) protein that is primarily expressed in human retinal pigment epithelium (RPEs). The GPCR-75 sequence analyses suggest the presence of 7 trans-membrane domains, a characteristic feature of GPCR. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3 intra cellular loop which might be the site for interaction of G-proteins. The short carboxy terminal is intracellular and has putative post-translational modification lipid modification sites.
Hum. Adult Placenta; Host:Rabbit. Target Name:GPR75. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; GPR75 antibody - middle region (ARP33103_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:GPR75. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; GPR75 antibody - middle region (ARP33103_P050) in Hum. Fetal Muscle cells using Western Blot
Human HepG2; WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; GPR75 antibody - middle region (ARP33103_P050) in Human HepG2 cells using Western Blot
Human COLO205; WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: COLO205 cell lysate. GPR75 is supported by BioGPS gene expression data to be expressed in COLO205.; GPR75 antibody - middle region (ARP33104_P050) in Human COLO205 cells using Western Blot
Human HeLa; WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:100. Positive Control: Hela cell lysate; GPR75 antibody - C-terminal region (ARP34088_P050) in Human HeLa cells using Western Blot
Immunofluorescent analysis of GPR75 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of GPR75 expression in HEK293T (A); A549 (B); SP2/0 (C); rat kidney (D) whole cell lysates.
Western blot analysis of extracts from HT-29 cells and K562 cells, using GPR75 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of LOVO cells, using GPR75 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: GPR75; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0ug/ml
Host: Rabbit; Target Name: GPR75; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: COLO205 cell lysateGPR75 is supported by BioGPS gene expression data to be expressed in COLO205
WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:100; Positive Control: Hela cell lysate
Anti-GPR75 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4-7 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4-7 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3-7 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3-7 ug/ml.
Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human brain, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 18 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 18 ug/ml.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315128 | GPR75 antibody |
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| Rabbit polyclonal anti-GPR75 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA340607 | GPR75 antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (C-Terminus) | |||||||
 |
Rabbit | Purified | Chk, Hu, Ms, Rt, Xen |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA340608 | GPR75 (C-term) antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (C-Terminus) | |||||||
 |
Rabbit | Aff - Purified | Chk, Elephant, Eq, Hu, L, Ms, Op, Por, Rb, Tr, Xen, Ze | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340652 | GPR75 (N-term Extracell. Dom.) antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (N-Terminus) | |||||||
|
Rabbit | Aff - Purified | Hu, Mky, Prim | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340653 | GPR75 antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (C-Terminus) | |||||||
 |
Rabbit | Purified | Bov, Hu, Mky, Por, Rb |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA340826 | GPR75 antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (Extracellular Domain) | |||||||
 |
Rabbit | Purified | Bov, Hst, Hu, Ms, Por, Rt |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA340827 | GPR75 (3rd cytopl. dom.) antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (Cytoplasmic Domain) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Elephant, Eq, Hu, Mky, Ms, Por, Rb, Rt, Xen | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340828 | GPR75 antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (C-Terminus) | |||||||
 |
Rabbit | Purified | Hst, Hu, Ms, Rt |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA340901 | GPR75 (2nd Cytopl. Dom.) antibody |
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| Rabbit Polyclonal Anti-GPR75 Antibody (Cytoplasmic Domain) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Por, Prim, Rb, Rt, Xen | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416418 | GPR75 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220478L2V | Lenti ORF particles, Gpr75 (GFP-tagged) - Mouse G protein-coupled receptor 75 (Gpr75), 200 uL, >10^7 TU/mL |
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200 µl /
€1,240.00
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| OriGene Technologies, Inc. | ||
| MR220478L1V | Lenti ORF particles, Gpr75 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 75 (Gpr75), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||