GRAP2 antibody
Principal name
GRAP2 antibody
Alternative names for GRAP2 antibody
GADS, GRBLG, GRB2L, Grf-40, GRBX, GRID, GRB2-related adapter protein 2, Growth factor receptor-binding protein, GRB-2-like protein, Grf40 adapter protein, Hematopoietic cell-associated adapter protein GrpL, Adapter protein GRID, SH3-SH2-SH3 adapter Mona
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F)
Background of GRAP2 antibody
In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-γ, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2. SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins. Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity. Gads is an adapter proteins that contains both SH2 and SH3 domains. Gads binds to tyrosine-phosphorylated proteins, such as Shc, and functions to couple these proteins to downstream effectors.
General readings
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Preisinger, C., et al. Cell. Signal. 22(5):848-856 (2010)
Davila, S., et al. Genes Immun. 11(3):232-238 (2010)
Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846 (2009)
Ludwig, L., et al. Cancer Lett. 275(2):194-197 (2009)
Immunoperoxidase of monoclonal antibody to GRAP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
Western Blot analysis of GRAP2 expression in transfected 293T cell line by GRAP2 monoclonal antibody (M01), clone 1G12.Lane 1: GRAP2 transfected lysate(38 KDa).Lane 2: Non-transfected lysate.
GRAP2 monoclonal antibody (M01), clone 1G12 Western Blot analysis of GRAP2 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged GRAP2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T01) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T02) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
HEK293 overexpressing GRAP2 and probed with GRAP2 polyclonal antibody ( Cat # PAB6036 ) ( mock transfection in first lane ), tested by Origene.
GRAP2 polyclonal antibody ( Cat # PAB6036 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Proximity Ligation Analysis (PLA) of protein-protein interactions between GRAP2 and CSF1R. HeLa cells were stained with anti-GRAP2 rabbit purified polyclonal 1:1200 and anti-CSF1R mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T03) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(37.90 KDa).Lane 2: Non-transfected lysate.
GRAP2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat.
GRAP2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat.
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T03) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(37.90 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of GRAP2 transfected lysate using anti-GRAP2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GRAP2 MaxPab mouse polyclonal antibody (B02) (H00009402-B02).
Western blot (WB) analysis of Gads antibody (Cat.-No.: AP21183PU-N) in extracts from HUVEC cells.
Recombinant human GRAP2, 1-330 aa, His-tagged
Western blot analysis of GRAP2 Antibody (Center) Cat.-No AP51943PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the GRAP2 antibody detected the GRAP2 protein (arrow).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (RC206546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
HEK293T cells transfected with either RC206546 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GRAP2 antibody(TA502923), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-GRAP2 antibody(TA502923),(Red), compared to a nonspecific negative control antibody,(Blue).
TA302501 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Sample (30 ug of whole cell lysate). A: JurKat. 10% SDS PAGE. TA308365 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Colon ca, using GRAP2(TA308365) antibody at 1:500 dilution.
Western blot analysis of extracts from HuvEc cells, using GRAP2 antibody.The lane on the right is treated with the synthesized peptide.
Western blot detection of GRAP2 in GRAP2 positive cell (CEM,Molt-4,Jurkat) and negative cell (3T3,K562,Hela) lysates using GRAP2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502923 | GRAP2 antibody |
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| GRAP2 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2) | |||||||
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Mouse | IgG1 | OTI1G2 | Purified | Hu | F, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
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| TA302501 | GRAP2 (C-term) antibody |
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| Goat Polyclonal Antibody against GRAP2 / GRID / Grf40 | |||||||
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Goat | Aff - Purified | Hu, Ms, Por | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA308365 | GRAP2 antibody |
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| Rabbit polyclonal antibody to GRAP2 (GRB2-related adaptor protein 2) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312173 | GRAP2 antibody |
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| Rabbit polyclonal anti-GRAP2 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA346954 | GRAP2 antibody |
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| Mouse Monoclonal GRAP2 Antibody | |||||||
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Mouse | IgG2b | 5C7-F4-E7 | Purified | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| AM26008PU-N | GRAP2 antibody |
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Mouse | IgG2a | UW40 | Aff - Purified | Hu | F, IP, WB |
0.1 mg /
€260.00
|
| OriGene Technologies GmbH | |||||||
| AP21183PU-N | GRAP2 antibody |
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Rabbit | Aff - Purified | Hu | WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| AP51943PU-N | GRAP2 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206546BN | GRAP2 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206546LP | GRAP2 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206546RB | GRAP2 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306546 | GRAP2 |
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| Recombinant protein of human GRB2-related adaptor protein 2 (GRAP2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204666L2V | Lenti ORF particles, Grap2 (GFP-tagged) - Mouse GRB2-related adaptor protein 2 (Grap2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR204666L1V | Lenti ORF particles, Grap2 (Myc-DDK-tagged) - Mouse GRB2-related adaptor protein 2 (Grap2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||
